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Spatiotemporal Regulation of a T4SS Substrate by the Metaeffector SidJ

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A key attribute of many pathogens is their ability to survive and replicate within eukaryotic host cells. One such pathogen, Legionella pneumophila, is able to grow within macrophages in the lungs, thereby causing a form of pneumonia called Legionnaires’ Disease. L. pneumophila causes disease by translocating several hundred proteins into the host cell. These proteins are typically referred to as ‘‘effectors’’, as they function as toxins to alter normal host cell function. However, since L. pneumophila remains within the host cells for approximately one day, continual poisoning of the eukaryotic cells by the bacterial effectors will result in the premature death of the host cell, thus restricting the growth of the pathogen. Previously the L. pneumophila secreted protein LubX was described as a “metaeffector”, which has been defined as an effector that acts directly on another effector to modulate its function inside the host cell. LubX accomplishes this task by directing the degradation of another effector, SidH. Here we report a second L. pneumophila metaeffector, SidJ, acts in a similar manner to neutralize SidE family effectors by removing them from the intracellular compartment that contains the bacterium. This further establishes the concept of metaeffectors, which are likely to be critical to how Legionella and many other pathogens cause disease.

Vyšlo v časopise: Spatiotemporal Regulation of a T4SS Substrate by the Metaeffector SidJ. PLoS Pathog 11(3): e32767. doi:10.1371/journal.ppat.1004695
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004695

Souhrn

A key attribute of many pathogens is their ability to survive and replicate within eukaryotic host cells. One such pathogen, Legionella pneumophila, is able to grow within macrophages in the lungs, thereby causing a form of pneumonia called Legionnaires’ Disease. L. pneumophila causes disease by translocating several hundred proteins into the host cell. These proteins are typically referred to as ‘‘effectors’’, as they function as toxins to alter normal host cell function. However, since L. pneumophila remains within the host cells for approximately one day, continual poisoning of the eukaryotic cells by the bacterial effectors will result in the premature death of the host cell, thus restricting the growth of the pathogen. Previously the L. pneumophila secreted protein LubX was described as a “metaeffector”, which has been defined as an effector that acts directly on another effector to modulate its function inside the host cell. LubX accomplishes this task by directing the degradation of another effector, SidH. Here we report a second L. pneumophila metaeffector, SidJ, acts in a similar manner to neutralize SidE family effectors by removing them from the intracellular compartment that contains the bacterium. This further establishes the concept of metaeffectors, which are likely to be critical to how Legionella and many other pathogens cause disease.

Zdroje

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