#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Pathogens / 2015 - 3

Dermal Neutrophil, Macrophage and Dendritic Cell Responses to Transmitted by Fleas

English version České info

Flea-borne transmission is central to the natural history of the plague bacillus Yersinia pestis, and infection within the context of flea feeding may affect the pathogenesis of bubonic plague. We analyzed the mammalian host response to Y. pestis in the skin immediately after transmission by its natural vector, the rat flea Xenopsylla cheopis, to observe differences relative to the response to needle-inoculated bacteria. Our results show that uninfected flea bites induce minimal inflammation, but flea-transmitted Y. pestis cause the recruitment of neutrophils roughly in proportion to the number of bacteria deposited in the skin. We observed interactions of flea-transmitted bacteria with macrophages, a cell type much more permissive than neutrophils for survival and growth of Y. pestis. We found that dendritic cells, important sentinel antigen presenting cells, were recruited to, but had minimal interaction with, flea-transmitted bacteria. Additionally, we found that Y. pestis could disseminate from the flea bite site to the draining lymph node and spleen as early as 1 h after flea feeding, significantly earlier than has been previously reported. This study reveals important differences between needle-inoculated and flea-transmitted Y. pestis in the immediate host response to infection and improves our understanding of the early host-bacterium interactions in plague pathogenesis.

Vyšlo v časopise: Dermal Neutrophil, Macrophage and Dendritic Cell Responses to Transmitted by Fleas. PLoS Pathog 11(3): e32767. doi:10.1371/journal.ppat.1004734
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004734

Souhrn

Flea-borne transmission is central to the natural history of the plague bacillus Yersinia pestis, and infection within the context of flea feeding may affect the pathogenesis of bubonic plague. We analyzed the mammalian host response to Y. pestis in the skin immediately after transmission by its natural vector, the rat flea Xenopsylla cheopis, to observe differences relative to the response to needle-inoculated bacteria. Our results show that uninfected flea bites induce minimal inflammation, but flea-transmitted Y. pestis cause the recruitment of neutrophils roughly in proportion to the number of bacteria deposited in the skin. We observed interactions of flea-transmitted bacteria with macrophages, a cell type much more permissive than neutrophils for survival and growth of Y. pestis. We found that dendritic cells, important sentinel antigen presenting cells, were recruited to, but had minimal interaction with, flea-transmitted bacteria. Additionally, we found that Y. pestis could disseminate from the flea bite site to the draining lymph node and spleen as early as 1 h after flea feeding, significantly earlier than has been previously reported. This study reveals important differences between needle-inoculated and flea-transmitted Y. pestis in the immediate host response to infection and improves our understanding of the early host-bacterium interactions in plague pathogenesis.

Zdroje

1. Butler T. Plague gives surprises in the first decade of the 21st century in the United States and worldwide. Am J Trop Med Hyg. 2013;89[4]:788–93. Epub 2013/09/18. doi: 10.4269/ajtmh.13-0191 24043686

2. Sebbane F, Jarrett CO, Gardner D, Long D, Hinnebusch BJ. Role of the Yersinia pestis plasminogen activator in the incidence of distinct septicemic and bubonic forms of flea-borne plague. Proc Natl Acad Sci USA. 2006;103[14]:5526–30. 16567636

3. Cui Y, Yu C, Yan Y, Li D, Li Y, Jombart T, et al. Historical variations in mutation rate in an epidemic pathogen, Yersinia pestis. Proc Natl Acad Sci U S A. 2013;110[2]:577–82. Epub 2012/12/29. doi: 10.1073/pnas.1205750110 23271803

4. Chouikha I, Hinnebusch BJ. Yersinia-flea interactions and the evolution of the arthropod-borne transmission route of plague. Current opinion in microbiology. 2012;15[3]:239–46. Epub 2012/03/13. doi: 10.1016/j.mib.2012.02.003 22406208

5. Hinnebusch BJ, Perry RD, Schwan TG. Role of the Yersinia pestis hemin storage (hms) locus in the transmission of plague by fleas. Science. 1996;273(5273):367–70. 8662526

6. Lorange EA, Race BL, Sebbane F, Hinnebusch BJ. Poor vector competence of fleas and the evolution of hypervirulence in Yersinia pestis. J Inf Dis. 2005;191[11]:1907–12. 15871125

7. Pujol C, Bliska JB. Turning Yersinia pathogenesis outside in: subversion of macrophage function by intracellular yersiniae. Clin Immunol. 2005;114[3]:216–26. 15721832

8. Spinner JL, Cundiff JA, Kobayashi SD. Yersinia pestis type III secretion system-dependent inhibition of human polymorphonuclear leukocyte function. Infect Immun. 2008;76[8]:3754–60. doi: 10.1128/IAI.00385-08 18490459

9. Spinner JL, Winfree S, Starr T, Shannon JG, Nair V, Steele-Mortimer O, et al. Yersinia pestis survival and replication within human neutrophil phagosomes and uptake of infected neutrophils by macrophages. Journal of leukocyte biology. 2013. Epub 2013/11/15.

10. Janssen WA, Surgalla MJ. Plague bacillus: survival within host phagocytes. Science. 1969;163(3870):950–2. Epub 1969/02/28. 5763880

11. Marketon MM, DePaolo RW, DeBord KL, Jabri B, Schneewind O. Plague bacteria target immune cells during infection. Science. 2005;309(5741):1739–41. 16051750

12. Bliska JB, Wang X, Viboud GI, Brodsky IE. Modulation of innate immune responses by Yersinia type III secretion system translocators and effectors. Cellular microbiology. 2013;15[10]:1622–31. Epub 2013/07/10. doi: 10.1111/cmi.12164 23834311

13. Cornelis GR, Wolf-Watz H. The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cells. Mol Microbiol. 1997;23[5]:861–7. 9076724

14. Vadyvaloo V, Jarrett C, Sturdevant DE, Sebbane F, Hinnebusch BJ. Transit through the flea vector induces a pretransmission innate immunity resistance phenotype in Yersinia pestis. PLoS Pathog. 2010;6[2]:e10000783.

15. Cavanaugh DC. Specific effect of temperature upon transmission of the plague bacillus by the oriental rat flea, Xenopsylla cheopis. Am J Trop Med Hyg. 1971;20[2]:264–73. 5553266

16. Shannon JG, Hasenkrug AM, Dorward DW, Nair V, Carmody AB, Hinnebusch BJ. Yersinia pestis subverts the dermal neutrophil response in a mouse model of bubonic plague. mBio. 2013;4[5]:e00170–13. Epub 2013/08/29. doi: 10.1128/mBio.00170-13 23982068

17. Andersen JF, Hinnebusch BJ, Lucas DA, Conrads TP, Veenstra TD, Pham VM, et al. An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots). BMC Genomics. 2007;8 : 102. 17437641

18. Peters NC, Egen JG, Secundino N, Debrabant A, Kimblin N, Kamhawi S, et al. In vivo imaging reveals an essential role for neutrophils in leishmaniasis transmitted by sand flies. Science. 2008;321(5891):970–4. Epub 2008/08/16. doi: 10.1126/science.1159194 18703742

19. Platt AM, Randolph GJ. Dendritic cell migration through the lymphatic vasculature to lymph nodes. Advances in immunology. 2013;120 : 51–68. Epub 2013/09/28. doi: 10.1016/B978-0-12-417028-5.00002-8 24070380

20. Lindquist RL, Shakhar G, Dudziak D, Wardemann H, Eisenreich T, Dustin ML, et al. Visualizing dendritic cell networks in vivo. Nat Immunol. 2004;5[12]:1243–50. Epub 2004/11/16. 15543150

21. Deoras PJ, Prasad RS. Feeding mechanism of Indian fleas X. cheopis (Roths) and X. astia (Roths). The Indian journal of medical research. 1967;55[10]:1041–50. Epub 1967/10/01. 5594375

22. Lavoipierre MMJ, Hamachi M. An apparatus for observations on the feeding mechanism of the flea. Nature. 1961;192 : 998–9.

23. Lammermann T, Afonso PV, Angermann BR, Wang JM, Kastenmuller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498(7454):371–5. Epub 2013/05/28. doi: 10.1038/nature12175 23708969

24. Spinner JL, Carmody AB, Jarrett CO, Hinnebusch BJ. Role of Yersinia pestis toxin complex family proteins in resistance to phagocytosis by polymorphonuclear leukocytes. Infect Immun. 2013;81[11]:4041–52. Epub 2013/08/21. doi: 10.1128/IAI.00648-13 23959716

25. Rebeil R, Jarrett CO, Driver JD, Ernst RK, Oyston PC, Hinnebusch BJ. Induction of the Yersinia pestis PhoP-PhoQ regulatory system in the flea and its role in producing a transmissible infection. J Bacteriol. 2013;195[9]:1920–30. Epub 2013/02/26. doi: 10.1128/JB.02000-12 23435973

26. Vuong C, Voyich JM, Fischer ER, Braughton KR, Whitney AR, DeLeo FR, et al. Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cellular microbiology. 2004;6[3]:269–75. Epub 2004/02/07. 14764110

27. Hume DA. Applications of myeloid-specific promoters in transgenic mice support in vivo imaging and functional genomics but do not support the concept of distinct macrophage and dendritic cell lineages or roles in immunity. Journal of leukocyte biology. 2011;89[4]:525–38. Epub 2010/12/21. doi: 10.1189/jlb.0810472 21169519

28. Kawai T, Akira S. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity. 2011;34[5]:637–50. Epub 2011/05/28. doi: 10.1016/j.immuni.2011.05.006 21616434

29. Jawetz E, Meyer KF. The behaviour of virulent and avirulent Pasteurella pestis in normal and immune experimental animals. J Inf Dis. 1944;74 : 1–13.

30. Sebbane F, Gardner D, Long D, Gowen BB, Hinnebusch BJ. Kinetics of disease progression and host response in a rat model of bubonic plague. Am J Pathol. 2005;166[5]:1427–39. 15855643

31. Gonzalez RJ, Lane MC, Wagner NJ, Weening EH, Miller VL. Dissemination of a highly virulent pathogen: tracking the early events that define infection. PLoS Pathog. 2015;11[1]:e1004587. doi: 10.1371/journal.ppat.1004587 25611317

32. Faust N, Varas F, Kelly LM, Heck S, Graf T. Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages. Blood. 2000;96[2]:719–26. 10887140

Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium
Článek To Be or Not IIb: A Multi-Step Process for Epstein-Barr Virus Latency Establishment and Consequences for B Cell Tumorigenesis
Článek Is Antigenic Sin Always “Original?” Re-examining the Evidence Regarding Circulation of a Human H1 Influenza Virus Immediately Prior to the 1918 Spanish Flu
Článek The Great Escape: Pathogen Versus Host
Článek Coping with Stress and the Emergence of Multidrug Resistance in Fungi
Článek Catch Me If You Can: The Link between Autophagy and Viruses
Článek Bacterial Immune Evasion through Manipulation of Host Inhibitory Immune Signaling
Článek Evidence for Ubiquitin-Regulated Nuclear and Subnuclear Trafficking among Matrix Proteins
Článek BILBO1 Is a Scaffold Protein of the Flagellar Pocket Collar in the Pathogen
Článek Production of Anti-LPS IgM by B1a B Cells Depends on IL-1β and Is Protective against Lung Infection with LVS
Článek Virulence Regulation with Venus Flytrap Domains: Structure and Function of the Periplasmic Moiety of the Sensor-Kinase BvgS
Článek α-Hemolysin Counteracts the Anti-Virulence Innate Immune Response Triggered by the Rho GTPase Activating Toxin CNF1 during Bacteremia
Článek Induction of Interferon-Stimulated Genes by IRF3 Promotes Replication of
Článek Intracellular Growth Is Dependent on Tyrosine Catabolism in the Dimorphic Fungal Pathogen
Článek HCV Induces the Expression of Rubicon and UVRAG to Temporally Regulate the Maturation of Autophagosomes and Viral Replication
Článek Spatiotemporal Analysis of Hepatitis C Virus Infection
Článek Subgingival Microbial Communities in Leukocyte Adhesion Deficiency and Their Relationship with Local Immunopathology
Článek Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway
Článek Attenuation of Tick-Borne Encephalitis Virus Using Large-Scale Random Codon Re-encoding
Článek Establishment of HSV1 Latency in Immunodeficient Mice Facilitates Efficient Reactivation
Článek XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability
Článek γδ T Cells Confer Protection against Murine Cytomegalovirus (MCMV)
Článek Rhadinovirus Host Entry by Co-operative Infection
Článek A Phospholipase Is Involved in Disruption of the Liver Stage Parasitophorous Vacuole Membrane
Článek Elucidation of Sigma Factor-Associated Networks in Reveals a Modular Architecture with Limited and Function-Specific Crosstalk
Článek A Conserved NS3 Surface Patch Orchestrates NS2 Protease Stimulation, NS5A Hyperphosphorylation and HCV Genome Replication
Článek Host ESCRT Proteins Are Required for Bromovirus RNA Replication Compartment Assembly and Function
Článek Disruption of IL-21 Signaling Affects T Cell-B Cell Interactions and Abrogates Protective Humoral Immunity to Malaria
Článek Compartmentalized Replication of R5 T Cell-Tropic HIV-1 in the Central Nervous System Early in the Course of Infection
Článek Diminished Reovirus Capsid Stability Alters Disease Pathogenesis and Littermate Transmission
Článek Characterization of CD8 T Cell Differentiation following SIVΔnef Vaccination by Transcription Factor Expression Profiling
Článek Visualization of HIV-1 Interactions with Penile and Foreskin Epithelia: Clues for Female-to-Male HIV Transmission
Článek Sensing Cytosolic RpsL by Macrophages Induces Lysosomal Cell Death and Termination of Bacterial Infection
Článek PKCη/Rdx-driven Phosphorylation of PDK1: A Novel Mechanism Promoting Cancer Cell Survival and Permissiveness for Parvovirus-induced Lysis
Článek Metalloprotease NleC Suppresses Host NF-κB/Inflammatory Responses by Cleaving p65 and Interfering with the p65/RPS3 Interaction
Článek Immune Antibodies and Helminth Products Drive CXCR2-Dependent Macrophage-Myofibroblast Crosstalk to Promote Intestinal Repair
Článek Adenovirus Entry From the Apical Surface of Polarized Epithelia Is Facilitated by the Host Innate Immune Response
Článek The RNA Template Channel of the RNA-Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family
Článek Neutrophils: Between Host Defence, Immune Modulation, and Tissue Injury
Článek CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies
Článek Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid β-Oxidation
Článek Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Infection
Článek TRIM26 Negatively Regulates Interferon-β Production and Antiviral Response through Polyubiquitination and Degradation of Nuclear IRF3
Článek Parallel Epigenomic and Transcriptomic Responses to Viral Infection in Honey Bees ()
Článek A Crystal Structure of the Dengue Virus NS5 Protein Reveals a Novel Inter-domain Interface Essential for Protein Flexibility and Virus Replication
Článek Enhanced CD8 T Cell Responses through GITR-Mediated Costimulation Resolve Chronic Viral Infection
Článek Exome and Transcriptome Sequencing of Identifies a Locus That Confers Resistance to and Alters the Immune Response
Článek The Role of Misshapen NCK-related kinase (MINK), a Novel Ste20 Family Kinase, in the IRES-Mediated Protein Translation of Human Enterovirus 71
Článek Chitin Recognition via Chitotriosidase Promotes Pathologic Type-2 Helper T Cell Responses to Cryptococcal Infection
Článek Activates Both IL-1β and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague
Článek Persistence of Transmitted HIV-1 Drug Resistance Mutations Associated with Fitness Costs and Viral Genetic Backgrounds
Článek An 18 kDa Scaffold Protein Is Critical for Biofilm Formation
Článek Early Virological and Immunological Events in Asymptomatic Epstein-Barr Virus Infection in African Children
Článek Human CD8 T-cells Recognizing Peptides from () Presented by HLA-E Have an Unorthodox Th2-like, Multifunctional, Inhibitory Phenotype and Represent a Novel Human T-cell Subset
Článek Decreased HIV-Specific T-Regulatory Responses Are Associated with Effective DC-Vaccine Induced Immunity
Článek RSV Vaccine-Enhanced Disease Is Orchestrated by the Combined Actions of Distinct CD4 T Cell Subsets
Článek Concerted Activity of IgG1 Antibodies and IL-4/IL-25-Dependent Effector Cells Trap Helminth Larvae in the Tissues following Vaccination with Defined Secreted Antigens, Providing Sterile Immunity to Challenge Infection
Článek Structure of the Low pH Conformation of Chandipura Virus G Reveals Important Features in the Evolution of the Vesiculovirus Glycoprotein
Článek PPM1A Regulates Antiviral Signaling by Antagonizing TBK1-Mediated STING Phosphorylation and Aggregation
Článek Lipidomic Analysis Links Mycobactin Synthase K to Iron Uptake and Virulence in .
Článek Roles and Programming of Arabidopsis ARGONAUTE Proteins during Infection
Článek Impact of Infection on Host Macrophage Nuclear Physiology and Nucleopore Complex Integrity
Článek The Impact of Host Diet on Titer in
Článek Antimicrobial-Induced DNA Damage and Genomic Instability in Microbial Pathogens
Článek Herpesviral G Protein-Coupled Receptors Activate NFAT to Induce Tumor Formation via Inhibiting the SERCA Calcium ATPase
Článek The Causes and Consequences of Changes in Virulence following Pathogen Host Shifts
Článek Small GTPase Rab21 Mediates Fibronectin Induced Actin Reorganization in : Implications in Pathogen Invasion
Článek Positive Role of Promyelocytic Leukemia Protein in Type I Interferon Response and Its Regulation by Human Cytomegalovirus
Článek NEDDylation Is Essential for Kaposi’s Sarcoma-Associated Herpesvirus Latency and Lytic Reactivation and Represents a Novel Anti-KSHV Target
Článek β-HPV 5 and 8 E6 Disrupt Homology Dependent Double Strand Break Repair by Attenuating BRCA1 and BRCA2 Expression and Foci Formation
Článek An O Antigen Capsule Modulates Bacterial Pathogenesis in
Článek Variable Processing and Cross-presentation of HIV by Dendritic Cells and Macrophages Shapes CTL Immunodominance and Immune Escape
Článek Probing the Metabolic Network in Bloodstream-Form Using Untargeted Metabolomics with Stable Isotope Labelled Glucose
Článek Adhesive Fiber Stratification in Uropathogenic Biofilms Unveils Oxygen-Mediated Control of Type 1 Pili
Článek Vaccinia Virus Protein Complex F12/E2 Interacts with Kinesin Light Chain Isoform 2 to Engage the Kinesin-1 Motor Complex
Článek Modulates Host Macrophage Mitochondrial Metabolism by Hijacking the SIRT1-AMPK Axis
Článek Human T-Cell Leukemia Virus Type 1 (HTLV-1) Tax Requires CADM1/TSLC1 for Inactivation of the NF-κB Inhibitor A20 and Constitutive NF-κB Signaling
Článek Suppression of RNAi by dsRNA-Degrading RNaseIII Enzymes of Viruses in Animals and Plants
Článek Spatiotemporal Regulation of a T4SS Substrate by the Metaeffector SidJ
Článek Antigenic Properties of the Human Immunodeficiency Virus Envelope Glycoprotein Gp120 on Virions Bound to Target Cells
Článek Dependence of Intracellular and Exosomal microRNAs on Viral Oncogene Expression in HPV-positive Tumor Cells
Článek Identification of a Peptide-Pheromone that Enhances Escape from Host Cell Vacuoles
Článek Impaired Systemic Tetrahydrobiopterin Bioavailability and Increased Dihydrobiopterin in Adult Falciparum Malaria: Association with Disease Severity, Impaired Microvascular Function and Increased Endothelial Activation
Článek Transgenic Expression of the Dicotyledonous Pattern Recognition Receptor EFR in Rice Leads to Ligand-Dependent Activation of Defense Responses
Článek Comprehensive Antigenic Map of a Cleaved Soluble HIV-1 Envelope Trimer
Článek Low Doses of Imatinib Induce Myelopoiesis and Enhance Host Anti-microbial Immunity
Článek Impaired Systemic Tetrahydrobiopterin Bioavailability and Increased Oxidized Biopterins in Pediatric Falciparum Malaria: Association with Disease Severity
Článok vyšiel v časopise

PLOS Pathogens

2015 Číslo 3
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

nový kurz
Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#