Preclinical Detection of Variant CJD and BSE Prions in Blood

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Variant Creutzfeldt Jakob Disease (vCJD) cases were identified in patients who received blood products that had been prepared from donors who later developed the disease. The blood borne transmission of vCJD is a major concern for blood transfusion banks, plasma derived products manufacturers and public health authorities. A vCJD blood screening test would represent an ideal solution for identifying donors/blood donations that might be at risk. In this study, we describe a blood assay which is based on the in vitro amplification of vCJD agent by Protein Misfolding Cyclic Amplification (PMCA). In vCJD animal models (sheep and primate), the assay enabled the identification of infected individuals in a very early stage of the asymptomatic incubation phase. We also provide evidence of the high specificity and the high analytical sensitivity of this assay using blood samples from vCJD affected and healthy patients.

Vyšlo v časopise: Preclinical Detection of Variant CJD and BSE Prions in Blood. PLoS Pathog 10(6): e32767. doi:10.1371/journal.ppat.1004202
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004202

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Zdroje

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