Kind Discrimination and Competitive Exclusion Mediated by Contact-Dependent Growth Inhibition Systems Shape Biofilm Community Structure

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Contact-Dependent Growth Inhibition (CDI) systems are highly diverse interbacterial competition systems that bacteria use to kill neighboring bacteria upon cell-cell contact. In Burkholderia species, BcpA is the large exoprotein responsible for mediating CDI. BcpI proteins provide immunity against auto-inhibition. Diversity of CDI systems exists within the toxic C-terminus of BcpA proteins (called the BcpA-CT) and BcpI proteins. In addition to mediating interbacterial competition in Burkholderia thailandensis, BcpA also mediates biofilm formation, suggesting CDI system proteins play a cooperative role in nature. However, the roles of CDI system-mediated interbacterial competition and of CDI system diversity in nature are unclear. We constructed B. thailandensis strains that produced different BcpA-CT and BcpI proteins. Bacteria participated in CDI during biofilm formation, resulting in biofilm structures that were segregated by CDI system protein types. Furthermore, competition via CDI allowed bacteria in a pre-established biofilm community producing one set of CDI system proteins to exclude bacteria producing a different set of CDI system proteins from entering the community. Our data imply, therefore, that CDI-mediated competition and CDI system diversity function as a mechanism for self-recognition during the development of microbial communities.

Vyšlo v časopise: Kind Discrimination and Competitive Exclusion Mediated by Contact-Dependent Growth Inhibition Systems Shape Biofilm Community Structure. PLoS Pathog 10(4): e32767. doi:10.1371/journal.ppat.1004076
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004076

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