Protection from the 2009 H1N1 Pandemic Influenza by an Antibody from Combinatorial Survivor-Based Libraries

English version České info

Influenza viruses elude immune responses and antiviral chemotherapeutics through genetic drift and reassortment. As a result, the development of new strategies that attack a highly conserved viral function to prevent and/or treat influenza infection is being pursued. Such novel broadly acting antiviral therapies would be less susceptible to virus escape and provide a long lasting solution to the evolving virus challenge. Here we report the in vitro and in vivo activity of a human monoclonal antibody (A06) against two isolates of the 2009 H1N1 pandemic influenza virus. This antibody, which was obtained from a combinatorial library derived from a survivor of highly pathogenic H5N1 infection, neutralizes H5N1, seasonal H1N1 and 2009 “Swine” H1N1 pandemic influenza in vitro with similar potency and is capable of preventing and treating 2009 H1N1 influenza infection in murine models of disease. These results demonstrate broad activity of the A06 antibody and its utility as an anti-influenza treatment option, even against newly evolved influenza strains to which there is limited immunity in the general population.

Vyšlo v časopise: Protection from the 2009 H1N1 Pandemic Influenza by an Antibody from Combinatorial Survivor-Based Libraries. PLoS Pathog 6(7): e32767. doi:10.1371/journal.ppat.1000990
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1000990

Souhrn

Zdroje

1. CohenJ

EnserinkM

2009 Swine flu. After delays, WHO agrees: the 2009 pandemic has begun. Science 324 1496 1497

2. ZarocostasJ

2009 World Health Organization declares A (H1N1) influenza pandemic. BMJ 338 b2425

3. AirGM

LaverWG

1989 The neuraminidase of influenza virus. Proteins 6 341 356

4. BoniMF

GogJR

AndreasenV

ChristiansenFB

2004 Influenza drift and epidemic size: the race between generating and escaping immunity. Theor Popul Biol 65 179 191

5. HillemanMR

2002 Realities and enigmas of human viral influenza: pathogenesis, epidemiology and control. Vaccine 20 3068 3087

6. ParkAW

DalyJM

LewisNS

SmithDJ

WoodJL

2009 Quantifying the impact of immune escape on transmission dynamics of influenza. Science 326 726 728

7. CasadevallA

2002 Passive antibody administration (immediate immunity) as a specific defense against biological weapons. Emerg Infect Dis 8 833 841

8. ChengLW

StankerLH

HendersonTD2nd

LouJ

MarksJD

2009 Antibody protection against botulinum neurotoxin intoxication in mice. Infect Immun 77 4305 4313

9. MeissnerHC

WelliverRC

ChartrandSA

LawBJ

WeismanLE

1999 Immunoprophylaxis with palivizumab, a humanized respiratory syncytial virus monoclonal antibody, for prevention of respiratory syncytial virus infection in high risk infants: a consensus opinion. Pediatr Infect Dis J 18 223 231

10. SawyerLA

2000 Antibodies for the prevention and treatment of viral diseases. Antiviral Res 47 57 77

11. LukeTC

KilbaneEM

JacksonJL

HoffmanSL

2006 Meta-analysis: convalescent blood products for Spanish influenza pneumonia: a future H5N1 treatment? Ann Intern Med 145 599 609

12. KashyapAK

SteelJ

OnerAF

DillonMA

SwaleRE

2008 Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies. Proc Natl Acad Sci U S A 105 5986 5991

13. SuiJ

HwangWC

PerezS

WeiG

AirdD

2009 Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses. Nat Struct Mol Biol 16 265 273

14. ThrosbyM

van den BrinkE

JongeneelenM

PoonLL

AlardP

2008 Heterosubtypic neutralizing monoclonal antibodies cross-protective against H5N1 and H1N1 recovered from human IgM+ memory B cells. PLoS One 3 e3942

15. ManicassamyB

MedinaRA

HaiR

TsibaneT

StertzS

Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccines. PLoS Pathog 6 e1000745

16. BaoY

BolotovP

DernovoyD

KiryutinB

ZaslavskyL

2008 The influenza virus resource at the National Center for Biotechnology Information. J Virol 82 596 601

17. EkiertDC

BhabhaG

ElsligerMA

FriesenRH

JongeneelenM

2009 Antibody recognition of a highly conserved influenza virus epitope. Science 324 246 251