Differential Responses to Wnt and PCP Disruption Predict Expression and Developmental Function of Conserved and Novel Genes in a Cnidarian

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The recent wave of genome sequencing from many species has revealed that most of the gene families known to regulate animal development are shared not only between humans and laboratory favorites such as mice, flies and worms, but also by evolutionarily more distant animals such as jellyfish and sponges. It is often assumed that genes inherited from a common ancestor remain largely responsible for regulating embryogenesis across these animal species, rather than more recently evolved genes. To address this issue we made an unbiased, systematic search for developmental genes in embryos of the jellyfish Clytia, selecting genes whose expression altered upon manipulation of the key regulator Wnt3, and comparing their expression in embryos specifically disrupted for Planar Cell Polarity. Identification of evolutionarily conserved and novel genes as developmental regulators was confirmed by demonstrating characteristic expression profiles for a sub-set of genes, and by gene knockdown studies. Conserved genes coded for members of many known signaling pathway and transcription factor families, as well as previously unstudied proteins. Nearly 30% of the identified genes were restricted to cnidarians (the jellyfish-sea anemone-coral group), supporting the idea that the appearance of new genes during evolution contributed significantly to generating animal diversity.

Vyšlo v časopise: Differential Responses to Wnt and PCP Disruption Predict Expression and Developmental Function of Conserved and Novel Genes in a Cnidarian. PLoS Genet 10(9): e32767. doi:10.1371/journal.pgen.1004590
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004590

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