Hidden Diversity in Honey Bee Gut Symbionts Detected by Single-Cell Genomics

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Gut microbial communities are often complex, consisting of bacteria from divergent phyla as well as multiple strains of each of the constituent species. But because the composition of these communities is typically assessed using 16S rRNA analyses, little is known about genomic changes associated with in situ diversification of bacterial lineages in animal guts. We undertook a single-cell genomic approach to investigate the diversification within two species of the gut microbiota of honey bees. Each species exhibited a surprisingly high level of genomic variation, despite uniformity in the 16S rRNA sequences. Our data indicate that genetically and ecologically distinct lineages can evolve in the gut of the same host species in the presence of frequent recombination at 16S rRNA genes. These findings parallel observations from mammals, suggesting that in situ diversification of a few bacterial lineages is a common pattern in the evolution of gut communities.

Vyšlo v časopise: Hidden Diversity in Honey Bee Gut Symbionts Detected by Single-Cell Genomics. PLoS Genet 10(9): e32767. doi:10.1371/journal.pgen.1004596
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004596

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