Local Effect of Enhancer of Zeste-Like Reveals Cooperation of Epigenetic and -Acting Determinants for Zygotic Genome Rearrangements

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The unicellular eukaryote Paramecium tetraurelia provides an extraordinary model for studying the mechanisms involved in zygotic genome rearrangements. At each sexual cycle, differentiation of the somatic nucleus from the zygotic nucleus is characterized by extensive remodeling of the entire somatic genome, which includes the precise excision of 45,000 short noncoding germline DNA segments to reconstitute functional open reading frames. Exploiting the unique properties of the Paramecium genome, we show that the enhancer of zeste like protein Ezl1 is necessary for histone H3 trimethylation on lysines 27 and 9 and is required for the precise excision of 31,000 of these single copy, dispersed germline DNA segments that can be as short as 26 bp in length. This implies that histone marks usually associated with heterochromatin may contribute to the precise demarcation of segments that are even shorter than the length of DNA wrapped around a single nucleosome. A quantitative analysis of high throughput sequencing datasets further shows that the underlying genetic properties of the germline DNA segments might act in concert with epigenetic signals to define germline specific sequences.

Vyšlo v časopise: Local Effect of Enhancer of Zeste-Like Reveals Cooperation of Epigenetic and -Acting Determinants for Zygotic Genome Rearrangements. PLoS Genet 10(9): e32767. doi:10.1371/journal.pgen.1004665
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004665

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