Role for Circadian Clock Genes in Seasonal Timing: Testing the Bünning Hypothesis

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The circadian clock consists of an extensive genetic network that drives daily rhythms of physiological, biochemical and behavioural processes. The network is evolutionary conserved and has been extensively studied in a broad range of organisms. Another genetic network constitutes the photoperiodic clock and monitors the seasonal change in day-length. Here, we address a major and long-standing question in chronobiology: whether the circadian clock is involved in photoperiodic timing, also known as the Bünning hypothesis. Drosophila, as with many other insects in temperate regions, exhibits a photoperiodic response that allows the insect to anticipate and survive the winter. Here we show that the cold-tolerance of the fly is regulated by the photoperiod. We use this phenotype to test day-length timing in various circadian clock mutants and observe that in null clock mutants, the photoperiodic response is abolished, whereas in mutants that exhibit short or long daily cycles, the photoperiodic response is modified, further supporting a circadian-clock function. Overall, these results provide the first evidence in Drosophila that support for the Bünning hypothesis, and pave the way for the genetic dissection of seasonal timing in Drosophila melanogaster.

Vyšlo v časopise: Role for Circadian Clock Genes in Seasonal Timing: Testing the Bünning Hypothesis. PLoS Genet 10(9): e32767. doi:10.1371/journal.pgen.1004603
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004603

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