Inhibition of bacterial cell wall synthesis by antibiotics such as penicillin can lead to unbalanced activity of a poorly defined set of lytic enzymes, termed ‘autolysins,’ which degrade the cell wall and typically cause cell lysis. Here, we report that in Vibrio cholerae (the cause of cholera), inhibition of cell wall synthesis results in the formation of viable spheres rather than cell lysis. Paradoxically, sphere formation requires the activity of cell wall degradative enzymes. Inhibition of cell wall synthesis in additional pathogens also leads to sphere formation. These findings expand our understanding of the cellular responses to cell wall acting antibiotics, demonstrating that cell wall degradative enzymes not only function as autolysins, but can also mediate cell survival in the face of cell wall insufficiency.
Vyšlo v časopise: Endopeptidase-Mediated Beta Lactam Tolerance. PLoS Pathog 11(4): e32767. doi:10.1371/journal.ppat.1004850 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004850
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