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Proteomic Analysis of the Acidocalcisome, an Organelle Conserved from Bacteria to Human Cells


Acidocalcisomes are acidic organelles conserved from bacteria to human cells that are rich in polyphosphate, a polymer of orthophosphate units linked by high-energy phospho-anyhidride bonds. We found here that acidocalcisomes from Trypanosoma brucei, belonging to the group of organisms that produces African sleeping sickness and nagana, are rich in pumps, channels, and transporters involved in phosphate and cation homeostasis, and calcium signaling. Proteomic analysis of acidocalcisome fractions and expression of genes with epitope tags validated the presence of a number of novel transporters, and RNA interference demonstrated the essentiality of these organelles.


Vyšlo v časopise: Proteomic Analysis of the Acidocalcisome, an Organelle Conserved from Bacteria to Human Cells. PLoS Pathog 10(12): e32767. doi:10.1371/journal.ppat.1004555
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004555

Souhrn

Acidocalcisomes are acidic organelles conserved from bacteria to human cells that are rich in polyphosphate, a polymer of orthophosphate units linked by high-energy phospho-anyhidride bonds. We found here that acidocalcisomes from Trypanosoma brucei, belonging to the group of organisms that produces African sleeping sickness and nagana, are rich in pumps, channels, and transporters involved in phosphate and cation homeostasis, and calcium signaling. Proteomic analysis of acidocalcisome fractions and expression of genes with epitope tags validated the presence of a number of novel transporters, and RNA interference demonstrated the essentiality of these organelles.


Zdroje

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