Loss of Dynamin-Related Protein 2B Reveals Separation of Innate Immune Signaling Pathways

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Plants have developed effective mechanisms for protection against pathogens including bacteria, but if a plant is unable to induce defenses, pathogenic bacteria invade and colonize the host, which can lead to reduced yield and nutritional quality of crops. An important aspect of engineering durable crop resistance against bacteria is elucidating and manipulating resistance pathways in the model plant Arabidopsis thaliana. The plant receptor FLAGELLIN SENSING 2 (FLS2) recognizes the bacterial protein flagellin to initiate host defense responses contributing to immunity. Here, we identify Dynamin-Related Protein 2B (DRP2B), previously implicated in membrane trafficking in plants, as a novel component of defense responses against flagellin and bacterial Pseudomonas syringae strains in Arabidopsis thaliana. More specifically, DRP2B functioned in the first line of defense against bacteria, namely in pattern-triggered immunity. We also demonstrated that DRP2B has different roles in three distinct branches of the flg22-signaling network that could be separated by their genetic requirement for the NADPH oxidase RbohD. In drp2b mutant plants, impaired ligand-induced endocytosis of FLS2 may contribute in part to the non-canonical combination of immune defects. Our findings highlight the importance of a functional vesicular trafficking network for plant immune responses and effective immunity against bacteria.

Vyšlo v časopise: Loss of Dynamin-Related Protein 2B Reveals Separation of Innate Immune Signaling Pathways. PLoS Pathog 10(12): e32767. doi:10.1371/journal.ppat.1004578
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004578

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