miR171-Targeted Scarecrow-Like Proteins Bind to GT -Elements and Mediate Gibberellin-Regulated Chlorophyll Biosynthesis under Light Conditions

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Chlorophyll biosynthesis is essential for plant growth and development. To date, the regulatory mechanisms of chlorophyll biosynthesis have been well understood only in dark conditions. Previous reports showed that miR171-targeted SCL6/22/27 proteins were involved in chlorophyll biosynthesis. However, the molecular mechanism of SCL action remains unclear. In this study, we found that SCLs negatively regulated chlorophyll biosynthesis though suppressing the expression of the key gene PROTOCHLOROPHYLLIDE OXIDOREDUCTASE (POR). SCL27 is highly expressed at the basal cell proliferation region of young leaves, suggesting an important role of SCLs in inhibiting chloroplast development before cell expansion. In addition, GT-cis elements were required for SCL27 directly binding to the PORC promoter. Furthermore, we showed that SCLs mediated GA-regulated chlorophyll biosynthesis through direct interaction with DELLA proteins. The interaction between SCLs and DELLAs reduced the DNA binding activity of SCL27. Our uncovered GA-DELLA-SCL module and its DNA binding targets provide new insights into molecular mechanisms by which chlorophyll biosynthesis and cell proliferation are coordinately regulated during leaf development in response to developmental and environmental cues.

Vyšlo v časopise: miR171-Targeted Scarecrow-Like Proteins Bind to GT -Elements and Mediate Gibberellin-Regulated Chlorophyll Biosynthesis under Light Conditions. PLoS Genet 10(8): e32767. doi:10.1371/journal.pgen.1004519
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004519

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