The Activation of Effector Avr3b by Plant Cyclophilin is Required for the Nudix Hydrolase Activity of Avr3b

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Phytophthora sojae, an oomycete pathogen that causes the Phytophthora root and stem rot disease of soybean, delivers variety of effectors into host cell to reprogram host immunity. Genome sequencing uncovers that P. sojae genome encode several hundreds of effector genes. However, the mode of action of most of the P. sojae effectors remains unknown. The investigation of effector-interacting proteins provides opportunities to better understand the pathogenesis mechanism of the pathogen and the defense mechanism of the host plants. Previously, we reported that P. sojae avirulence effector Avr3b modulates plant immunity through its Nudix hydrolase activity. Interestingly, the enzymatic activity is required for its virulence but not required for recognition by resistant gene. In this study, we identified soybean cyclophilin protein GmCYP1 as an Avr3b interactor. The enzymatic activity of GmCYP1 is required for the maturation Avr3b, which is directly related to both virulence and avirulence functions of Avr3b. This work provides a novel insight into how Phytophthora pathogens recruit host proteins to activate the enzymatic activity of effectors in order to gain successful infection.

Vyšlo v časopise: The Activation of Effector Avr3b by Plant Cyclophilin is Required for the Nudix Hydrolase Activity of Avr3b. PLoS Pathog 11(8): e32767. doi:10.1371/journal.ppat.1005139
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005139

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