EhVps32 Is a Vacuole-Associated Protein Involved in Pinocytosis and Phagocytosis of

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Trophozoites of E.
histolytica represent an excellent model to study endosomal-sorting complex required for transport components due to their high endocytic activity and vesicle trafficking. The key role of EhVps32 on phagocytosis is supported by: i) its presence on phagosomes, ii) its interaction with EhADH (an erythrocytes receptor), Gal/GalNac lectin and actin, iii) the higher rate of erythrophagocytosis showed by EhVps32 overexpressing trophozoites, iv) the diminish rate of phagocytosis in EhVps32-silenced G3 trophozoites, and v) its location in erythrocytes-containing acidic phagosomes. Here, we discovered the presence of membranous concentric helicoidally and tunnel-like structures constituted by EhVps32 and EhADH that may have a dynamic role in membrane remodeling and in the generation of intraluminal vesicles in the phagosomes. Elucidating molecular mechanisms of endocytosis-exocytosis pathways will help us to better understand the pathogenic process of E. histolytica and develop new drugs for diagnosis and vaccine methods.

Vyšlo v časopise: EhVps32 Is a Vacuole-Associated Protein Involved in Pinocytosis and Phagocytosis of. PLoS Pathog 11(7): e32767. doi:10.1371/journal.ppat.1005079
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1005079

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