Contribution of Coagulases towards Disease and Protective Immunity

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The bacterial pathogen Staphylococcus aureus seeds abscesses in host tissues to replicate at the center of these lesions, protected from host immune cells via a pseudocapsule. Using histochemical staining, we identified prothrombin and fibrin within abscesses and pseudocapsules. S. aureus secretes two clotting factors, coagulase (Coa) and von Willebrand factor binding protein (vWbp). We report here that Coa and vWbp together are required for the formation of abscesses. Coa and vWbp promote the non-proteolytic activation of prothrombin and cleavage of fibrinogen, reactions that are inhibited with specific antibody against each of these molecules. Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.

Vyšlo v časopise: Contribution of Coagulases towards Disease and Protective Immunity. PLoS Pathog 6(8): e32767. doi:10.1371/journal.ppat.1001036
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1001036

Souhrn

Zdroje

1. LowyFD

1998 Staphylococcus aureus infections. New Engl J Med 339 520 532

2. KlevensRM

EdwardsJR

GaynesRP

SystemNNIS

2008 The impact of antimicrobial-resistant, health care-associated infections on mortality in the United States. Clin Infect Dis 47 927 930

3. RogersDE

MellyMA

1965 Speculation on the immunology of Mudd, S. Capsulation, pseudocapsulation, and the somatic antigens of the surface of Staphylococcus aureus. Ann NY Acad Sci 128 45 56

4. ProjanSJ

NesinM

DunmanPM

2006 Staphylococcal vaccines and immunotherapy: to dream the impossible dream? Curr Opin Pharmacol 6 473 479

5. ChengAG

KimHK

BurtsML

KrauszT

SchneewindO

2009 Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J 23 1 12

6. MazmanianSK

SkaarEP

GasparAH

HumayunM

GornickiP

2003 Passage of heme-iron across the envelope of Staphylococcus aureus. Science 299 906 909

7. ThammavongsaV

KernJW

MissiakasDM

SchneewindO

2009 Staphylococcus aureus synthesizes adenosine to escape host immune responses. J Exp Med 206 2417 2427

8. GoodyearCS

SilvermanGJ

2003 Death by a B cell superantigen: In vivo VH-targeted apoptotic supraclonal B cell deletion by a staphylococcal toxin. J Exp Med 197 1125 1139

9. Stranger-JonesYK

BaeT

SchneewindO

2006 Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Nat Acad Sci USA 103 16942 16947

10. KuklinNA

ClarkDJ

SecoreS

CookJ

CopeLD

2006 A novel Staphylococcus aureus vaccine: iron surface determinant B induces rapid antibody responses in rhesus macaques and specific increased survival in a murine S. aureus sepsis model. Infect Immun 74 2215 2223

11. KimHK

ChengAG

KimH-Y

MissiakasDM

SchneewindO

2010 Non-toxigenic protein A vaccine for methicillin-resistant Staphylococcus aureus infections. J Exp Med under review

12. MuchH

1908 Über eine Vorstufe des Fibrinfermentes in Kulturen von Staphylokokkus aureus. Biochem Z 14 143

13. LoebL

1903 The influence of certain bacteria on the coagulation of blood. J Med Res 10 407

14. FieldHI

SmithHW

1945 Coagulase test for staphylococci. J Comp Pathol 55 63

15. SmithW

HaleJH

SmithMM

1947 The role of coagulase in staphylococcal infections. Brit J Exp Pathol 28 57

16. PhonimdaengP

O'ReillyM

NowlanP

BramleyAJ

FosterTJ

1990 The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol 4 393 404

17. KinoshitaM

KobayashiN

NagashimaS

IshinoM

OtokozawaS

2008 Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus. Microbiol Immunols 52 334 348

18. DuthieES

LorenzLL

1952 Staphylococcal coagulase: mode of action and antigenicity. J Gen Microbiol 6 95 107

19. PanizziP

FriedrichR

Fuentes-PriorP

RichterK

BockPE

2006 Fibrinogen substrate recognition by staphylocoagulase (pro)thrombin complexes. J Biol Chem 281 1179 1187

20. FriedrichR

PanizziP

Fuentes-PriorP

RichterK

VerhammeI

2003 Staphylocoagulase is a prototype for the mechanism of cofactor-induced zymogen activation. Nature 425 535 539

21. TagerM

DrummondMC

1965 Staphylocoagulase. Ann NY Acad Sci 128 92 111

22. BjerketorpJ

NilssonM

LjunghA

FlockJI

JacobssonK

2002 A novel von Willebrand factor binding protein expressed by Staphylococcus aureus. Microbiology 148 2037 2044

23. BjerketorpJ

JacobssonK

FrykbergL

2004 The von Willebrand factor-binding protein (vWbp) of Staphylococcus aureus is a coagulase. FEMS Microbiol Lett 234 309 314

24. KrohHK

PanizziP

BockPE

2009 von Willebrand factor-binding protein is a hysteretic conformational activator of prothrombin. Proc Natl Acad Sci USA 106 7786 7791

25. BabaT

BaeT

SchneewindO

TakeuchiF

HiramatsuK

2007 Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes. J Bacteriol 190 300 310

26. AlbusA

ArbeitRD

LeeJC

1991 Virulence studies of Staphylococcus aureus mutants altered in type 5 capsule production. Infect Immun 59 1008 1014

27. RothforkJM

Dessus-BabusS

van WamelWJB

CheungAL

GreshamH

2003 Fibrinogen depletion attenuates Staphylococcus aureus infection by preventing density-dependent virulence gene up-regulation. J Immunol 171 5389 5395

28. SilbermanS

BernikM

OPotterE

KwuanHC

1973 Effects of ancrod (Arvin) in mice: studies of plasma fibrinogen and fibrinolytic activity. Br J Haematol 24 101 113

29. BaeT

SchneewindO

2005 Allelic replacement in Staphylococcus aureus with inducible counter-selection. Plasmid 55 58 63

30. SchneewindO

Mihaylova-PetkovD

ModelP

1993 Cell wall sorting signals in surface protein of Gram-positive bacteria. EMBO 12 4803 4811

31. SchneewindO

ModelP

FischettiVA

1992 Sorting of protein A to the staphylococcal cell wall. Cell 70 267 281

32. HarveyRP

DegryseE

StefaniL

SchamberF

CazenaveJP

1986 Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis. Proc Natl Acad Sci USA 83 1084 1088

33. MarkwardtF

1955 Untersuchungen über Hirudin. Naturwissenschaften 41 537 538

34. KlevensRM

MorrisonMA

NadleJ

PetitS

GershmanK

2007 Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 298 1763 1771

35. DeBordKL

AndersonDM

MarketonMM

OverheimKA

DePaoloRW

2006 Immunogenicity and protective immunity against bubonic and pneumonic plague by immunization of mice with the recombinant V10 antigen, a variant of LcrV. Infect Immun 74 4910 4914

36. PanizziP

FriedrichR

Fuentes-PriorP

RichterK

BockPE

2006 Fibrinogen substrate recognition by staphylocoagulase.(pro)thrombin complexes. J Biol Chem 281 1179 1187

37. KennedyAD

OttoM

BraughtonKR

WhitneyAR

ChenL

2008 Epidemic community-associated methicillin-resistant Staphylococcus aureus: recent clonal expansion and diversification. Proc Natl Acad Sci U S A 105 1327 1332

38. EkstedtRD

YotisWD

1960 Effect if coagulase on the virulence of coagulase-negative strains. Ann NY Acad Sci 80 496 500

39. MoreillonP

EntenzaJM

FrancioliP

McDevittD

FosterTJ

1995 Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis. Infect Immun 63 4738 4743

40. MainieroM

GoerkeC

GeigerT

GonserC

HerbertS

2010 Differential target gene activation by the Staphylococcus aureus two-component system saeRS. J Bacteriol 192 613 623

41. Birch-HirschfeldL

1934 Über die Agglutination von Staphylokokken durch Bestandteile des Säugetierblutplasmas. Klinische Woschenschrift 13 331

42. McDevittD

FrancoisP

VaudauxP

FosterTJ

1994 Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol Microbiol 11 237 248

43. Ní EidhinD

PerkinsS

FrancoisP

VaudauxP

HöökM

1998 Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol Microbiol 30 245 257

44. GeogheganJA

GaneshVK

SmedsE

LiangX

HookM

2010 Molecular characterization of the interaction of staphylococcal MSCRAMMS ClfA and Fbl with fibrinogen. J Biol Chem Epub ahead of print

45. PalmqvistN

JosefssonE

TarkowskiA

2004 Clumping factor A-mediated virulence during Staphylococcus aureus infection is retained despite fibrinogen depletion. Microbes Infect 6 196 201

46. JosefssonE

HigginsJ

FosterTJ

TarkowskiA

2008 Fibrinogen binding sites P336 and Y338 of clumping factor A are crucial for Staphylococcus aureus virulence. PLoS One 3 e2206

47. PalmaM

WadeD

FlockM

FlockJI

1998 Multiple binding sites in the interaction between an extracellular fibrinogen-binding protein from Staphylococcus aureus and fibrinogen. J Biol Chem 273 13177 13181

48. HeilmannC

HerrmannM

KehrelBE

PetersG

2002 Platelet-binding domains in 2 fibrinogen-binding proteins of Staphylococcus aureus identified by phage display. J Infect Dis 186 32 39

49. HussainM

BeckerK

von EiffC

SchrenzelJ

PetersG

2001 Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins. J Bacteriol 183 6778 6786

50. WatanabeS

ItoT

SasakiT

LiS

UchiyamaI

2009 Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable virulence factors in Staphylococcus aureus. PLoS One 4 e5714

51. StreitfeldMM

SallmanB

ShoelsonSM

1959 Staphylococagulase inhibition by pooled human gamma-globulin. Nature 184 1665 1666

52. StudierFW

RosenbergAH

DunnJJ

DubendorffJW

1990 Use of T7 polymerase to direct expression of cloned genes. Methods Enzymol 185 60 89