#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Pathogens / 2010 - 8

Characterization of Oseltamivir-Resistant 2009 H1N1 Pandemic Influenza A Viruses

English version České info

Influenza viruses resistant to antiviral drugs emerge frequently. Not surprisingly, the widespread treatment in many countries of patients infected with 2009 pandemic influenza A (H1N1) viruses with the neuraminidase (NA) inhibitors oseltamivir and zanamivir has led to the emergence of pandemic strains resistant to these drugs. Sporadic cases of pandemic influenza have been associated with mutant viruses possessing a histidine-to-tyrosine substitution at position 274 (H274Y) in the NA, a mutation known to be responsible for oseltamivir resistance. Here, we characterized in vitro and in vivo properties of two pairs of oseltaimivir-sensitive and -resistant (possessing the NA H274Y substitution) 2009 H1N1 pandemic viruses isolated in different parts of the world. An in vitro NA inhibition assay confirmed that the NA H274Y substitution confers oseltamivir resistance to 2009 H1N1 pandemic viruses. In mouse lungs, we found no significant difference in replication between oseltamivir-sensitive and -resistant viruses. In the lungs of mice treated with oseltamivir or even zanamivir, 2009 H1N1 pandemic viruses with the NA H274Y substitution replicated efficiently. Pathological analysis revealed that the pathogenicities of the oseltamivir-resistant viruses were comparable to those of their oseltamivir-sensitive counterparts in ferrets. Further, the oseltamivir-resistant viruses transmitted between ferrets as efficiently as their oseltamivir-sensitive counterparts. Collectively, these data indicate that oseltamivir-resistant 2009 H1N1 pandemic viruses with the NA H274Y substitution were comparable to their oseltamivir-sensitive counterparts in their pathogenicity and transmissibility in animal models. Our findings highlight the possibility that NA H274Y-possessing oseltamivir-resistant 2009 H1N1 pandemic viruses could supersede oseltamivir-sensitive viruses, as occurred with seasonal H1N1 viruses.

Vyšlo v časopise: Characterization of Oseltamivir-Resistant 2009 H1N1 Pandemic Influenza A Viruses. PLoS Pathog 6(8): e32767. doi:10.1371/journal.ppat.1001079
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1001079

Souhrn

Influenza viruses resistant to antiviral drugs emerge frequently. Not surprisingly, the widespread treatment in many countries of patients infected with 2009 pandemic influenza A (H1N1) viruses with the neuraminidase (NA) inhibitors oseltamivir and zanamivir has led to the emergence of pandemic strains resistant to these drugs. Sporadic cases of pandemic influenza have been associated with mutant viruses possessing a histidine-to-tyrosine substitution at position 274 (H274Y) in the NA, a mutation known to be responsible for oseltamivir resistance. Here, we characterized in vitro and in vivo properties of two pairs of oseltaimivir-sensitive and -resistant (possessing the NA H274Y substitution) 2009 H1N1 pandemic viruses isolated in different parts of the world. An in vitro NA inhibition assay confirmed that the NA H274Y substitution confers oseltamivir resistance to 2009 H1N1 pandemic viruses. In mouse lungs, we found no significant difference in replication between oseltamivir-sensitive and -resistant viruses. In the lungs of mice treated with oseltamivir or even zanamivir, 2009 H1N1 pandemic viruses with the NA H274Y substitution replicated efficiently. Pathological analysis revealed that the pathogenicities of the oseltamivir-resistant viruses were comparable to those of their oseltamivir-sensitive counterparts in ferrets. Further, the oseltamivir-resistant viruses transmitted between ferrets as efficiently as their oseltamivir-sensitive counterparts. Collectively, these data indicate that oseltamivir-resistant 2009 H1N1 pandemic viruses with the NA H274Y substitution were comparable to their oseltamivir-sensitive counterparts in their pathogenicity and transmissibility in animal models. Our findings highlight the possibility that NA H274Y-possessing oseltamivir-resistant 2009 H1N1 pandemic viruses could supersede oseltamivir-sensitive viruses, as occurred with seasonal H1N1 viruses.

Zdroje

1. DawoodFS

JainS

FinelliL

ShawMW

LindstromS

2009 Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 360 2605 2615

2. ItohY

ShinyaK

KisoM

WatanabeT

SakodaY

2009 In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses. Nature 460 1021 1025

3. MunsterVJ

de WitE

van den BrandJM

HerfstS

SchrauwenEJ

2009 Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets. Science 325 481 483

4. MainesTR

JayaramanA

BelserJA

WadfordDA

PappasC

2009 Transmission and pathogenesis of swine-origin 2009 A(H1N1) influenza viruses in ferrets and mice. Science 325 484 487

5. JainS

KamimotoL

BramleyAM

SchmitzAM

BenoitSR

2009 Hospitalized patients with 2009 H1N1 influenza in the United States, April-June 2009. N Engl J Med 361 1935 1944

6. Centers for Disease Control and Prevention 2009 Update: drug susceptibility of swine-origin influenza A (H1N1) viruses, April 2009. MMWR Morb Mortal Wkly Rep 58 433 435

7. GubarevaLV

KaiserL

HaydenFG

2000 Influenza virus neuraminidase inhibitors. Lancet 355 827 835

8. KisoM

MitamuraK

Sakai-TagawaY

ShiraishiK

KawakamiC

2004 Resistant influenza A viruses in children treated with oseltamivir: descriptive study. Lancet 364 759 765

9. ZambonM

HaydenFG

2001 Position statement: global neuraminidase inhibitor susceptibility network. Antiviral Res 49 147 156

10. WardP

SmallI

SmithJ

SuterP

DutkowskiR

2005 Oseltamivir (Tamiflu) and its potential for use in the event of an influenza pandemic. J Antimicrob Chemother 55 Suppl 1 i5 i21

11. LeQM

KisoM

SomeyaK

SakaiYT

NguyenTH

2005 Avian flu: isolation of drug-resistant H5N1 virus. Nature 437 1108

12. Centers for Disease Control and Prevention 2009 Oseltamivir-resistant novel influenza A (H1N1) virus infection in two immunosuppressed patients -⁠ Seattle, Washington, 2009. MMWR Morb Mortal Wkly Rep 58 893 896

13. LeungTW

TaiAL

ChengPK

KongMS

LimW

2009 Detection of an oseltamivir-resistant pandemic influenza A/H1N1 virus in Hong Kong. J Clin Virol 46 298 299

14. LeQM

WertheimHF

TranND

van DoornHR

NguyenTH

2010 A community cluster of oseltamivir-resistant cases of 2009 H1N1 influenza. N Engl J Med 362 86 87

15. YamashitaM

TomozawaT

KakutaM

TokumitsuA

NasuH

2009 CS-8958, a prodrug of the new neuraminidase inhibitor R-125489, shows long-acting anti-influenza virus activity. Antimicrob Agents Chemother 53 186 192

16. NeumannG

NodaT

KawaokaY

2009 Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 459 931 939

17. IsonMG

MishinVP

BracialeTJ

HaydenFG

GubarevaLV

2006 Comparative activities of oseltamivir and A-322278 in immunocompetent and immunocompromised murine models of influenza virus infection. J Infect Dis 193 765 772

18. IlyushinaNA

BovinNV

WebsterRG

GovorkovaEA

2006 Combination chemotherapy, a potential strategy for reducing the emergence of drug-resistant influenza A variants. Antiviral Res 70 121 131

19. NguyenJT

HoopesJD

LeMH

SmeeDF

PatickAK

Triple combination of amantadine, ribavirin, and oseltamivir is highly active and synergistic against drug resistant influenza virus strains in vitro. PLoS One 5 e9332

20. IlyushinaNA

HayA

YilmazN

BoonAC

WebsterRG

2008 Oseltamivir-ribavirin combination therapy for highly pathogenic H5N1 influenza virus infection in mice. Antimicrob Agents Chemother 52 3889 3897

21. GovorkovaEA

FangHB

TanM

WebsterRG

2004 Neuraminidase inhibitor-rimantadine combinations exert additive and synergistic anti-influenza virus effects in MDCK cells. Antimicrob Agents Chemother 48 4855 4863

22. MorrisonD

RoyS

RaynerC

AmerA

HowardD

2007 A randomized, crossover study to evaluate the pharmacokinetics of amantadine and oseltamivir administered alone and in combination. PLoS ONE 2 e1305

23. HaugeSH

DudmanS

BorgenK

LackenbyA

HungnesO

2009 Oseltamivir-resistant influenza viruses A (H1N1), Norway, 2007-08. Emerg Infect Dis 15 155 162

24. NicollA

CiancioB

KramarzP

2008 Observed oseltamivir resistance in seasonal influenza viruses in Europe interpretation and potential implications. Euro Surveill 13

25. LackenbyA

ThompsonCI

DemocratisJ

2008 The potential impact of neuraminidase inhibitor resistant influenza. Curr Opin Infect Dis 21 626 638

26. HondaT

KuboS

MasudaT

AraiM

KobayashiY

2009 Synthesis and in vivo influenza virus-inhibitory effect of ester prodrug of 4-guanidino-7-O-methyl-Neu5Ac2en. Bioorg Med Chem Lett 19 2938 2940

27. GubarevaLV

WebsterRG

HaydenFG

2001 Comparison of the activities of zanamivir, oseltamivir, and RWJ-270201 against clinical isolates of influenza virus and neuraminidase inhibitor-resistant variants. Antimicrob Agents Chemother 45 3403 3408

28. HatakeyamaS

SugayaN

ItoM

YamazakiM

IchikawaM

2007 Emergence of influenza B viruses with reduced sensitivity to neuraminidase inhibitors. Jama 297 1435 1442

Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium
Článek Dissecting the Genetic Architecture of Host–Pathogen Specificity
Článek The Battle for Iron between Bacterial Pathogens and Their Vertebrate Hosts
Článek Global Genotype-Phenotype Correlations in
Článek Burkholderia Type VI Secretion Systems Have Distinct Roles in Eukaryotic and Bacterial Cell Interactions
Článek Chitin Synthases from Are Involved in Tip Growth and Represent a Potential Target for Anti-Oomycete Drugs
Článek Distinct Merkel Cell Polyomavirus Molecular Features in Tumour and Non Tumour Specimens from Patients with Merkel Cell Carcinoma
Článek Biological and Structural Characterization of a Host-Adapting Amino Acid in Influenza Virus
Článek Functional Characterisation and Drug Target Validation of a Mitotic Kinesin-13 in
Článek CTCF Prevents the Epigenetic Drift of EBV Latency Promoter Qp
Článek The Human Fungal Pathogen Escapes Macrophages by a Phagosome Emptying Mechanism That Is Inhibited by Arp2/3 Complex-Mediated Actin Polymerisation
Článek Bim Nuclear Translocation and Inactivation by Viral Interferon Regulatory Factor
Článek Cyst Wall Protein 1 Is a Lectin That Binds to Curled Fibrils of the GalNAc Homopolymer
Článek Reciprocal Analysis of Infections of a Model Reveal Host-Pathogen Conflicts Mediated by Reactive Oxygen and imd-Regulated Innate Immune Response
Článek A Subset of Replication Proteins Enhances Origin Recognition and Lytic Replication by the Epstein-Barr Virus ZEBRA Protein
Článek Damaged Intestinal Epithelial Integrity Linked to Microbial Translocation in Pathogenic Simian Immunodeficiency Virus Infections
Článek Kaposin-B Enhances the PROX1 mRNA Stability during Lymphatic Reprogramming of Vascular Endothelial Cells by Kaposi's Sarcoma Herpes Virus
Článek Direct Interaction between Two Viral Proteins, the Nonstructural Protein 2C and the Capsid Protein VP3, Is Required for Enterovirus Morphogenesis
Článek A Novel CCR5 Mutation Common in Sooty Mangabeys Reveals SIVsmm Infection of CCR5-Null Natural Hosts and Efficient Alternative Coreceptor Use
Článek Micro RNAs of Epstein-Barr Virus Promote Cell Cycle Progression and Prevent Apoptosis of Primary Human B Cells
Článek Enterohemorrhagic Requires N-WASP for Efficient Type III Translocation but Not for EspF-Mediated Actin Pedestal Formation
Článek Host Imprints on Bacterial Genomes—Rapid, Divergent Evolution in Individual Patients
Článek UNC93B1 Mediates Host Resistance to Infection with
Článek The Transcription Factor Rbf1 Is the Master Regulator for -Mating Type Controlled Pathogenic Development in
Článek Protective Efficacy of Cross-Reactive CD8 T Cells Recognising Mutant Viral Epitopes Depends on Peptide-MHC-I Structural Interactions and T Cell Activation Threshold
Článek Bacteriophage Lysin Mediates the Binding of to Human Platelets through Interaction with Fibrinogen
Článek Insecticide Control of Vector-Borne Diseases: When Is Insecticide Resistance a Problem?
Článek Immune Modulation with Sulfasalazine Attenuates Immunopathogenesis but Enhances Macrophage-Mediated Fungal Clearance during Pneumonia
Článek PKC Signaling Regulates Drug Resistance of the Fungal Pathogen via Circuitry Comprised of Mkc1, Calcineurin, and Hsp90
Článek A Multi-Step Process of Viral Adaptation to a Mutagenic Nucleoside Analogue by Modulation of Transition Types Leads to Extinction-Escape
Článek “Everything You Always Wanted to Know about Sex (but Were Afraid to Ask)” in after Two Decades of Laboratory and Field Analyses
Článek Norovirus Gastroenteritis, Carbohydrate Receptors, and Animal Models
Článek Variations in TcdB Activity and the Hypervirulence of Emerging Strains of
Článek SWAN-1 Binds to EGL-9 and Regulates HIF-1-Mediated Resistance to the Bacterial Pathogen PAO1
Článek Conformational Adaptation of Asian Macaque TRIMCyp Directs Lineage Specific Antiviral Activity
Článek The Proteasome Active Site Threonine Is Essential for Persistence Yet Dispensable for Replication and Resistance to Nitric Oxide
Článek The Pneumococcal Serine-Rich Repeat Protein Is an Intra-Species Bacterial Adhesin That Promotes Bacterial Aggregation and in Biofilms
Článek Regulatory T Cell Suppressive Potency Dictates the Balance between Bacterial Proliferation and Clearance during Persistent Infection
Článek Structural Alterations in a Component of Cytochrome Oxidase and Molecular Evolution of Pathogenic in Humans
Článek A Limited Number of Antibody Specificities Mediate Broad and Potent Serum Neutralization in Selected HIV-1 Infected Individuals
Článek Spliced Leader Trapping Reveals Widespread Alternative Splicing Patterns in the Highly Dynamic Transcriptome of
Článek Contribution of Coagulases towards Disease and Protective Immunity
Článek Early Severe Inflammatory Responses to Uropathogenic Predispose to Chronic and Recurrent Urinary Tract Infection
Článok vyšiel v časopise

PLOS Pathogens

2010 Číslo 8
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

nový kurz
Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#