The Endosymbiotic Bacterium Induces Resistance to Dengue Virus in

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Genetic strategies that reduce or block pathogen transmission by mosquitoes have been proposed as a means of augmenting current control measures to reduce the growing burden of vector-borne diseases. The endosymbiotic bacterium Wolbachia has long been promoted as a potential vehicle for introducing disease-resistance genes into mosquitoes, thereby making them refractory to the human pathogens they transmit. Given the large overlap in tissue distribution and intracellular localization between Wolbachia and dengue virus in mosquitoes, we conducted experiments to characterize their interactions. Our results show that Wolbachia inhibits viral replication and dissemination in the main dengue vector, Aedes aegypti. Moreover, the virus transmission potential of Wolbachia-infected Ae. aegypti was significantly diminished when compared to wild-type mosquitoes that did not harbor Wolbachia. At 14 days post-infection, Wolbachia completely blocked dengue transmission in at least 37.5% of Ae. aegypti mosquitoes. We also observed that this Wolbachia-mediated viral interference was associated with an elevated basal immunity and increased longevity in the mosquitoes. These results underscore the potential usefulness of Wolbachia-based control strategies for population replacement.

Vyšlo v časopise: The Endosymbiotic Bacterium Induces Resistance to Dengue Virus in. PLoS Pathog 6(4): e32767. doi:10.1371/journal.ppat.1000833
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1000833

Souhrn

Zdroje

1. GublerDJ

KunoG

1997 Dengue and Dengue Hemorrhagic Fever New York Cab International

2. FranzAW

Sanchez-VargasI

AdelmanZN

BlairCD

BeatyBJ

2006 Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti. Proc Natl Acad Sci U S A 103 4198 4203

3. OlsonKE

HiggsS

GainesPJ

PowersAM

DavisBS

1996 Genetically engineered resistance to dengue-2 virus transmission in mosquitoes. Science 272 884 886

4. ChenCH

HuangH

WardCM

SuJT

SchaefferLV

2007 A synthetic maternal-effect selfish genetic element drives population replacement in Drosophila. Science 316 597 600

5. SinkinsSP

GouldF

2006 Gene drive systems for insect disease vectors. Nat Rev Genet 7 427 435

6. XiZ

KhooCC

DobsonSL

2005 Wolbachia establishment and invasion in an Aedes aegypti laboratory population. Science 310 326 328

7. GublerDJ

ClarkGG

1995 Dengue/dengue hemorrhagic fever: the emergence of a global health problem. Emerg Infect Dis 1 55 57

8. SalazarMI

RichardsonJH

Sanchez-VargasI

OlsonKE

BeatyBJ

2007 Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes. BMC Microbiol 7 9

9. MinKT

BenzerS

1997 Wolbachia, normally a symbiont of Drosophila, can be virulent, causing degeneration and early death. Proceedings of the National Academy of Sciences of the United States of America 94 10792 10796

10. McMenimanCJ

LaneRV

CassBN

FongAW

SidhuM

2009 Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti. Science 323 141 144

11. DobsonSL

BourtzisK

BraigHR

JonesBF

ZhouW

1999 Wolbachia infections are distributed throughout insect somatic and germ line tissues. Insect Biochem Mol Biol 29 153 160

12. HedgesLM

BrownlieJC

O'NeillSL

JohnsonKN

2008 Wolbachia and virus protection in insects. Science 322 702

13. TeixeiraL

FerreiraA

AshburnerM

2008 The bacterial symbiont Wolbachia induces resistance to RNA viral infections in Drosophila melanogaster. PLoS Biol 6 e2 doi:10.1371/journal.pbio.1000002

14. XiZ

RamirezJL

DimopoulosG

2008 The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathog 4 e1000098 doi:10.1371/journal.ppat.1000098

15. XiZ

GavotteL

XieY

DobsonSL

2008 Genome-wide analysis of the interaction between the endosymbiotic bacterium Wolbachia and its Drosophila host. BMC Genomics 9 1

16. WattG

KantipongP

de SouzaM

ChanbancherdP

JongsakulK

2000 HIV-1 suppression during acute scrub-typhus infection. Lancet 356 475 479

17. De GregorioE

SpellmanPT

TzouP

RubinGM

LemaitreB

2002 The Toll and Imd pathways are the major regulators of the immune response in Drosophila. Embo J 21 2568 2579

18. HoffmannJA

2003 The immune response of Drosophila. Nature 426 33 38

19. ZambonRA

NandakumarM

VakhariaVN

WuLP

2005 The Toll pathway is important for an antiviral response in Drosophila. Proc Natl Acad Sci U S A 102 7257 7262

20. MoreiraLA

Iturbe-OrmaetxeI

JefferyJA

LuG

PykeAT

2009 A Wolbachia symbiont in Aedes aegypti limits infection with dengue, Chikungunya, and Plasmodium. Cell 139 1268 1278

21. DobsonSL

MarslandEJ

RattanadechakulW

2002 Mutualistic Wolbachia infection in Aedes albopictus: accelerating cytoplasmic drive. Genetics 160 1087 1094

22. IkeyaT

BroughtonS

AlicN

GrandisonR

PartridgeL

2009 The endosymbiont Wolbachia increases insulin/IGF-like signalling in Drosophila. Proc Biol Sci 276 3799 3807

23. TroyerJM

HanleyKA

WhiteheadSS

StrickmanD

KarronRA

2001 A live attenuated recombinant dengue-4 virus vaccine candidate with restricted capacity for dissemination in mosquitoes and lack of transmission from vaccinees to mosquitoes. Am J Trop Med Hyg 65 414 419

24. Molina-CruzA

GuptaL

RichardsonJ

BennettK

BlackWt

2005 Effect of mosquito midgut trypsin activity on dengue-2 virus infection and dissemination in Aedes aegypti. Am J Trop Med Hyg 72 631 637

25. TortosaP

CourtiolA

MoutaillerS

FaillouxA

WeillM

2008 Chikungunya-Wolbachia interplay in Aedes albopictus. Insect Molecular Biology 17 677 684