#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Pathogens / 2010 - 4

Topology and Organization of the Type III Secretion Needle Complex Components

English version České info

The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biology of many pathogenic or symbiotic bacteria. A central component of T3SSs is the needle complex, a multiprotein structure that mediates the passage of effector proteins through the bacterial envelope. We have used cryo electron microscopy combined with bacterial genetics, site-specific labeling, mutational analysis, chemical derivatization and high-resolution mass spectrometry to generate an experimentally validated topographic map of a Salmonella typhimurium T3SS needle complex. This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis.

Vyšlo v časopise: Topology and Organization of the Type III Secretion Needle Complex Components. PLoS Pathog 6(4): e32767. doi:10.1371/journal.ppat.1000824
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1000824

Souhrn

The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biology of many pathogenic or symbiotic bacteria. A central component of T3SSs is the needle complex, a multiprotein structure that mediates the passage of effector proteins through the bacterial envelope. We have used cryo electron microscopy combined with bacterial genetics, site-specific labeling, mutational analysis, chemical derivatization and high-resolution mass spectrometry to generate an experimentally validated topographic map of a Salmonella typhimurium T3SS needle complex. This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis.

Zdroje

1. CianciottoNP

2005 Type II secretion: a protein secretion system for all seasons. Trends Microbiol 13 581 588

2. JohnsonTL

AbendrothJ

HolWG

SandkvistM

2006 Type II secretion: from structure to function. FEMS Microbiol Lett 255 175 186

3. LlosaM

RoyC

DehioC

2009 Bacterial type IV secretion systems in human disease. Mol Microbiol 73 141 151

4. FillouxA

HachaniA

BlevesS

2008 The bacterial type VI secretion machine: yet another player for protein transport across membranes. Microbiology 154 1570 1583

5. GalanJE

Wolf-WatzH

2006 Protein delivery into eukaryotic cells by type III secretion machines. Nature 444 567 573

6. KuboriT

1998 Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science 280 602 665

7. SekiyaK

2001 Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure. Proc Natl Acad Sci U S A 98 11638 11643

8. BlockerA

2001 Structure and composition of the Shigella flexneri “needle complex”, a part of its type III secreton. Mol Microbiol 39 652 663

9. MarlovitsTC

2004 Structural insights into the assembly of the type III secretion needle complex. Science (New York, NY) 306 1040 1042

10. SukhanA

KuboriT

WilsonJ

GalanJE

2001 Genetic analysis of assembly of the Salmonella enterica serovar Typhimurium type III secretion-associated needle complex. J Bacteriol 183 1159 1167

11. MarlovitsTC

2006 Assembly of the inner rod determines needle length in the type III secretion injectisome. Nature 441 637 640

12. SukhanA

KuboriT

GalanJE

2003 Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ. J Bacteriol 185 3480 343

13. CrepinVF

2005 Structural and functional studies of the enteropathogenic Escherichia coli type III needle complex protein EscJ. Mol Microbiol 55 1658 1670

14. YipCK

2005 Structural characterization of the molecular platform for type III secretion system assembly. Nature 435 702 707

15. SpreterT

2009 A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system. Nat Struct Mol Biol 16 468 476

16. HodgkinsonJL

2009 Three-dimensional reconstruction of the Shigella T3SS transmembrane regions reveals 12-fold symmetry and novel features throughout. Nat Struct Mol Biol 16 477 485

17. HardieKR

LoryS

PugsleyAP

1996 Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J 15 978 988

18. KosterM

1997 The outer membrane component, YscC, of the Yop secretion machinery of Yersinia enterocolitica forms a ring-shaped multimeric complex. Mol Microbiol 26 789 797

19. CollinsRF

DavidsenL

DerrickJP

FordRC

TonjumT

2001 Analysis of the PilQ secretin from Neisseria meningitidis by transmission electron microscopy reveals a dodecameric quaternary structure. Journal of Bacteriology 183 3825 3832

20. LinderothNA

SimonMN

RusselM

1997 The filamentous phage pIV multimer visualized by scanning transmission electron microscopy. Science 278 1635 1638

21. ChamiM

2005 Structural Insights into the Secretin PulD and Its Trypsin-resistant Core. J Biol Chem 280 37732 37741

22. KanigaK

BossioJC

GalanJE

1994 The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins. Mol Microbiol 13 555 568

23. KimbroughTG

MillerSI

2000 Contribution of Salmonella typhimurium type III secretion components to needle complex formation. Proc Natl Acad Sci U S A 97 11008 1113

24. MindellJA

GrigorieffN

2003 Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol 142 334 347

25. RinnerO

2008 Identification of cross-linked peptides from large sequence databases. Nat Methods 5 315 318

Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium
Článek Innate Recognition of Fungal Cell Walls
Článek Suppression of mRNAs Encoding Tegument Tetraspanins from Results in Impaired Tegument Turnover
Článek Junín Virus Infection of Human Hematopoietic Progenitors Impairs Proplatelet Formation and Platelet Release via a Bystander Effect Involving Type I IFN Signaling
Článek The Endosymbiotic Bacterium Induces Resistance to Dengue Virus in
Článek Natural Regulatory T Cells in Malaria: Host or Parasite Allies?
Článek Keratinocytes Determine Th1 Immunity during Early Experimental Leishmaniasis
Článek Spatial and Temporal Association of Outbreaks of H5N1 Influenza Virus Infection in Wild Birds with the 0°C Isotherm
Článek Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine-Related Metabolic Pathways
Článek RNA Polymerase Activity and Specific RNA Structure Are Required for Efficient HCV Replication in Cultured Cells
Článek The Physical Relationship between Infectivity and Prion Protein Aggregates Is Strain-Dependent
Článek Inadequate Clearance of Translocated Bacterial Products in HIV-Infected Humanized Mice
Článek Temperature Modulates Plant Defense Responses through NB-LRR Proteins
Článek Peptide Inhibitors of Dengue-Virus Entry Target a Late-Stage Fusion Intermediate
Článek Identification of Host-Dependent Survival Factors for Intracellular through an siRNA Screen
Článek Exposure to HIV-1 Directly Impairs Mucosal Epithelial Barrier Integrity Allowing Microbial Translocation
Článek Increased Asymmetric Dimethylarginine in Severe Falciparum Malaria: Association with Impaired Nitric Oxide Bioavailability and Fatal Outcome
Článek Reconstitution of SARS-Coronavirus mRNA Cap Methylation
Článek Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8
Článek Two Coregulated Efflux Transporters Modulate Intracellular Heme and Protoporphyrin IX Availability in
Článek The Type I NADH Dehydrogenase of Counters Phagosomal NOX2 Activity to Inhibit TNF-α-Mediated Host Cell Apoptosis
Článek Rhomboid 4 (ROM4) Affects the Processing of Surface Adhesins and Facilitates Host Cell Invasion by
Článek Increased Monocyte Turnover from Bone Marrow Correlates with Severity of SIV Encephalitis and CD163 Levels in Plasma
Článek The RING-CH Ligase K5 Antagonizes Restriction of KSHV and HIV-1 Particle Release by Mediating Ubiquitin-Dependent Endosomal Degradation of Tetherin
Článek Molecular Mechanisms of Ethanol-Induced Pathogenesis Revealed by RNA-Sequencing
Článek Highly Frequent Mutations in Negative Regulators of Multiple Virulence Genes in Group A Streptococcal Toxic Shock Syndrome Isolates
Článek Emergence and Pathogenicity of Highly Virulent Genotypes in the Northwest United States
Článek Structural and Functional Analysis of Viral siRNAs
Článek Prion Shedding from Olfactory Neurons into Nasal Secretions
Článek a GATA Transcription Factor That Directs Disparate Fates in Including Morphogenesis and Siderophore Biosynthesis
Článek Three Members of the 6-cys Protein Family of Play a Role in Gamete Fertility
Článek Complement as an Endogenous Adjuvant for Dendritic Cell-Mediated Induction of Retrovirus-Specific CTLs
Článek A Genomic Survey of Positive Selection in Provides Insights into the Evolution of Accidental Virulence
Článek Overcomes Stress of Azole Drugs by Formation of Disomy in Specific Multiple Chromosomes
Článek Blood Fluke Exploitation of Non-Cognate CD4 T Cell Help to Facilitate Parasite Development
Článek Antagonism of Tetherin Restriction of HIV-1 Release by Vpu Involves Binding and Sequestration of the Restriction Factor in a Perinuclear Compartment
Článek The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
Článek Deficiencies in Jasmonate-Mediated Plant Defense Reveal Quantitative Variation in Pathogenesis
Článek Interactions with Bacteria in the Context of Human Health and Disease
Článek Viral Capsid Is a Pathogen-Associated Molecular Pattern in Adenovirus Keratitis
Článek Electron Tomography Reveals the Steps in Filovirus Budding
Článek Selective Condensation Drives Partitioning and Sequential Secretion of Cyst Wall Proteins in Differentiating
Článek The Effect of Vaccination on the Evolution and Population Dynamics of Avian Paramyxovirus-1
Článek A Timescale for Evolution, Population Expansion, and Spatial Spread of an Emerging Clone of Methicillin-Resistant
Článek VacA Toxin/Subunit p34: Targeting of an Anion Channel to the Inner Mitochondrial Membrane
Článek Production of Extracellular Traps against and in Infected Lung Tissue Is Dependent on Invading Neutrophils and Influenced by Hydrophobin RodA
Článek A Differential Role for Macropinocytosis in Mediating Entry of the Two Forms of Vaccinia Virus into Dendritic Cells
Článek Impaired Innate Immunity in Mice but Preserved CD8 T Cell Responses against in -, -, - or -Deficient Mice
Článek SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism
Článek Proteolysis of Human Thrombin Generates Novel Host Defense Peptides
Článek Multilayered Mechanism of CD4 Downregulation by HIV-1 Vpu Involving Distinct ER Retention and ERAD Targeting Steps
Článok vyšiel v časopise

PLOS Pathogens

2010 Číslo 4
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#