Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype

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When RNA viruses replicate, they do so with a high rate of error; hence, their populations are not composed of a single genotype, but of a swarm of different, yet related, genomes. This mutant spectrum has been described as the viral quasispecies, and its composition has important consequences for evolution, adaptation and emergence. In this study, we analysed adaptation in fine detail thanks to the use of the deep sequencing, and we determined the adaptative pathway of a model RNA virus, Coxsackievirus B3, to a new environment, A549 cells. Our results demonstrate that adaptation occurred in response to a differential expression of the virus receptors in the new cellular environment, compared to the former. Our experiments and mathematical analyses established that the corresponding increase in fitness resulted from the selection and contribution of a group of genotypes, including low frequency variants, and not to the effect of a single, dominant genome. Our work underscores the importance of considering group effects when studying RNA virus biology and evolution.

Vyšlo v časopise: Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. PLoS Pathog 11(5): e32767. doi:10.1371/journal.ppat.1004838
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004838

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