Transition of Sporozoites into Liver
Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio
Many eukaryotic developmental and cell fate decisions that are effected
post-transcriptionally involve RNA binding proteins as regulators of translation
of key mRNAs. In malaria parasites (Plasmodium spp.), the
development of round, non-motile and replicating exo-erythrocytic liver stage
forms from slender, motile and cell-cycle arrested sporozoites is believed to
depend on environmental changes experienced during the transmission of the
parasite from the mosquito vector to the vertebrate host. Here we identify a
Plasmodium member of the RNA binding protein family PUF as
a key regulator of this transformation. In the absence of Pumilio-2 (Puf2)
sporozoites initiate EEF development inside mosquito salivary glands
independently of the normal transmission-associated environmental cues.
Puf2- sporozoites exhibit genome-wide transcriptional
changes that result in loss of gliding motility, cell traversal ability and
reduction in infectivity, and, moreover, trigger metamorphosis typical of early
Plasmodium intra-hepatic development. These data
demonstrate that Puf2 is a key player in regulating sporozoite developmental
control, and imply that transformation of salivary gland-resident sporozoites
into liver stage-like parasites is regulated by a post-transcriptional
mechanism.
Vyšlo v časopise:
Transition of Sporozoites into Liver
Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio. PLoS Pathog 7(5): e32767. doi:10.1371/journal.ppat.1002046
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1002046
Souhrn
Many eukaryotic developmental and cell fate decisions that are effected
post-transcriptionally involve RNA binding proteins as regulators of translation
of key mRNAs. In malaria parasites (Plasmodium spp.), the
development of round, non-motile and replicating exo-erythrocytic liver stage
forms from slender, motile and cell-cycle arrested sporozoites is believed to
depend on environmental changes experienced during the transmission of the
parasite from the mosquito vector to the vertebrate host. Here we identify a
Plasmodium member of the RNA binding protein family PUF as
a key regulator of this transformation. In the absence of Pumilio-2 (Puf2)
sporozoites initiate EEF development inside mosquito salivary glands
independently of the normal transmission-associated environmental cues.
Puf2- sporozoites exhibit genome-wide transcriptional
changes that result in loss of gliding motility, cell traversal ability and
reduction in infectivity, and, moreover, trigger metamorphosis typical of early
Plasmodium intra-hepatic development. These data
demonstrate that Puf2 is a key player in regulating sporozoite developmental
control, and imply that transformation of salivary gland-resident sporozoites
into liver stage-like parasites is regulated by a post-transcriptional
mechanism.
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Hygiena a epidemiológia Infekčné lekárstvo LaboratóriumČlánok vyšiel v časopise
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