RNA Processing Factors Swd2.2 and Sen1 Antagonize RNA Pol III-Dependent Transcription and the Localization of Condensin at Pol III Genes

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Failure to condense chromosomes prior to anaphase onset can lead to genome instability. The evolutionary-conserved condensin complex drives chromosome condensation, probably by changing the topology of chromatin around its binding sites. Condensin localizes to regions of high transcription, suggesting that some transcription-associated feature(s) direct its association with chromatin. Here we considered that transcription-dependent DNA:RNA hybrids or topological stress could be involved in recruiting condensin. Our data show that condensin is indeed enriched at regions accumulating DNA:RNA hybrids but that they are not involved in its recruitment. Rather, we identify a mutant combination where increased transcription by RNA Pol III is associated locally with stronger topological stress. Strikingly the localization of condensin is dramatically enhanced at the same loci and we show that topological stress contributes to this enhanced association. Our data strengthen the idea that transcription creates the environment necessary to recruit condensin in mitosis.

Vyšlo v časopise: RNA Processing Factors Swd2.2 and Sen1 Antagonize RNA Pol III-Dependent Transcription and the Localization of Condensin at Pol III Genes. PLoS Genet 10(11): e32767. doi:10.1371/journal.pgen.1004794
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004794

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