Recognition and Activation Domains Contribute to Allele-Specific Responses of an Arabidopsis NLR Receptor to an Oomycete Effector Protein

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Plants defend themselves against pathogens using specific multi-domain immune receptors, which are able to recognize secreted “effector” proteins from the pathogen, and thus activate an immune response. Variants of the Arabidopsis immune receptor RPP1 recognize different alleles of the oomycete effector ATR1 through direct association. RPP1 and ATR1 alleles from different ecotypes and strains show a spectrum of recognition phenotypes, reflecting coevolution by the plant and pathogen to evade and re-establish immunity. In this work, we identified mutations in an unrecognized ATR1 allele that lead to allele-specific recognition by RPP1. Using chimeric constructs of the immune receptor, in which domains were swapped between two alleles, we were able to determine domains contributing to allele-specific activation. Our data point to the involvement of two domains in specific activation of immune receptors—one to associate with the effector, and one to sensitize the receptor and facilitate activation. We suggest that these domains must cooperate to efficiently and specifically recognize pathogen effectors. As NLRs confer pathogen resistance in many crop species, characterizing specificity domains involved in effector recognition will inform future efforts to breed or engineer disease resistant varieties.

Vyšlo v časopise: Recognition and Activation Domains Contribute to Allele-Specific Responses of an Arabidopsis NLR Receptor to an Oomycete Effector Protein. PLoS Pathog 11(2): e32767. doi:10.1371/journal.ppat.1004665
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004665

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