#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Pathogens / 2011 - 9

Hostile Takeover by : Reorganization of Parasite and Host Cell Membranes during Liver Stage Egress

English version České info

The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoerythrocytic merozoites into membrane-surrounded vesicles, called merosomes, which are delivered directly into liver sinusoids. However, it was unclear whether the membrane of these merosomes was derived from the parasite membrane, the parasitophorous vacuole membrane or the host cell membrane. This knowledge is required to determine how phagocytes will be directed against merosomes. Here, we fluorescently label the candidate membranes and use live cell imaging to show that the merosome membrane derives from the host cell membrane. We also demonstrate that proteins in the host cell membrane are lost during merozoite liberation from the parasitophorous vacuole. Immediately after the breakdown of the parasitophorous vacuole membrane, the host cell mitochondria begin to degenerate and protein biosynthesis arrests. The intact host cell plasma membrane surrounding merosomes allows Plasmodium to mask itself from the host immune system and bypass the numerous Kupffer cells on its way into the bloodstream. This represents an effective strategy for evading host defenses before establishing a blood stage infection.

Vyšlo v časopise: Hostile Takeover by : Reorganization of Parasite and Host Cell Membranes during Liver Stage Egress. PLoS Pathog 7(9): e32767. doi:10.1371/journal.ppat.1002224
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1002224

Souhrn

The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoerythrocytic merozoites into membrane-surrounded vesicles, called merosomes, which are delivered directly into liver sinusoids. However, it was unclear whether the membrane of these merosomes was derived from the parasite membrane, the parasitophorous vacuole membrane or the host cell membrane. This knowledge is required to determine how phagocytes will be directed against merosomes. Here, we fluorescently label the candidate membranes and use live cell imaging to show that the merosome membrane derives from the host cell membrane. We also demonstrate that proteins in the host cell membrane are lost during merozoite liberation from the parasitophorous vacuole. Immediately after the breakdown of the parasitophorous vacuole membrane, the host cell mitochondria begin to degenerate and protein biosynthesis arrests. The intact host cell plasma membrane surrounding merosomes allows Plasmodium to mask itself from the host immune system and bypass the numerous Kupffer cells on its way into the bloodstream. This represents an effective strategy for evading host defenses before establishing a blood stage infection.

Zdroje

1. WHO 2010 Fact Sheet Malaria. WHO Media Centre Fact sheet No 94: www.who.int/mediacentre/factsheets/fs094/en/

2. SturmAAminoRvan de SandCRegenTRetzlaffS 2006 Manipulation of host hepatocytes by the malaria parasite for delivery into liver sinusoids. Science 313 1287 1290

3. BaerKKlotzCKappeSHSchniederTFrevertU 2007 Release of hepatic Plasmodium yoelii merozoites into the pulmonary microvasculature. PLoS Pathog 3 e171

4. GreenwoodBMFidockDAKyleDEKappeSHAlonsoPL 2008 Malaria: progress, perils, and prospects for eradication. J Clin Invest 118 1266 1276

5. PurvesWK 1998 Life The Science of Biology. New York  W.H. Freeman & Company

6. KimYEChenJChanJRLangenR 2009 Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processes and degeneration. Nat Methods 7 67 73

7. HelmSLehmannCNagelAStanwayRRHorstmannS 2010 Identification and characterization of a liver stage-specific promoter region of the malaria parasite Plasmodium. PLoS One 5 e13653

8. StanwayRRGraeweSRennenbergAHelmSHeusslerVT 2009 Highly efficient subcloning of rodent malaria parasites by injection of single merosomes or detached cells. Nat Protoc 4 1433 1439

9. CollinsJCWolkoffAWMorellAGStockertRJ 1988 Selective regulation of intrinsic membrane proteins in HepG2. Hepatology 8 108 115

10. RamosRRSwansonAJBassJ 2007 Calreticulin and Hsp90 stabilize the human insulin receptor and promote its mobility in the endoplasmic reticulum. Proc Natl Acad Sci U S A 104 10470 10475

11. Schneider-PoetschTJuJEylerDEDangYBhatS 2010 Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat Chem Biol 6 209 217

12. JeffreyIWBushellMTillerayVJMorleySClemensMJ 2002 Inhibition of protein synthesis in apoptosis: differential requirements by the tumor necrosis factor alpha family and a DNA-damaging agent for caspases and the double-stranded RNA-dependent protein kinase. Cancer Res 62 2272 2280

13. van de SandCHorstmannSSchmidtASturmABolteS 2005 The liver stage of Plasmodium berghei inhibits host cell apoptosis. Mol Microbiol 58 731 742

14. PartikianAOlveczkyBSwaminathanRLiYVerkmanAS 1998 Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J Cell Biol 140 821 829

15. StanwayRRMuellerNZobiakBGraeweSFroehlkeU 2011 Organelle segregation into Plasmodium liver stage merozoites. Cellular Microbiology  in press

16. JanseCJRamesarJWatersAP 2006 High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei. Nat Protoc 1 346 356

17. SturmAGraeweSFranke-FayardBRetzlaffSBolteS 2009 Alteration of the parasite plasma membrane and the parasitophorous vacuole membrane during exo-erythrocytic development of malaria parasites. Protist 160 51 63

18. MeisJFVerhaveJPJapPHMeuwissenJH 1985 Fine structure of exoerythrocytic merozoite formation of Plasmodium berghei in rat liver. J Protozool 32 694 699

19. IshinoTBoissonBOritoYLacroixCBischoffE 2009 LISP1 is important for the egress of Plasmodium berghei parasites from liver cells. Cell Microbiol 11 1329 1339

20. MoudyRManningTJBeckersCJ 2001 The loss of cytoplasmic potassium upon host cell breakdown triggers egress of Toxoplasma gondii. J Biol Chem 276 41492 41501

21. NagamuneKHicksLMFuxBBrossierFChiniEN 2008 Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Nature 451 207 210

22. KafsackBFPenaJDCoppensIRavindranSBoothroydJC 2009 Rapid membrane disruption by a perforin-like protein facilitates parasite exit from host cells. Science 323 530 533

23. BlackmanMJ 2008 Malarial proteases and host cell egress: an ‘emerging’ cascade. Cell Microbiol 10 1925 1934

24. YeohSO'DonnellRAKoussisKDluzewskiARAnsellKH 2007 Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. Cell 131 1072 1083

25. TangHLLungHLWuKCLeAHTangHM 2008 Vimentin supports mitochondrial morphology and organization. Biochem J 410 141 146

26. FrankSGaumeBBergmann-LeitnerESLeitnerWWRobertEG 2001 The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Dev Cell 1 515 525

27. KroemerGReedJC 2000 Mitochondrial control of cell death. Nat Med 6 513 519

28. MullerAGuntherDDuxFNaumannMMeyerTF 1999 Neisserial porin (PorB) causes rapid calcium influx in target cells and induces apoptosis by the activation of cysteine proteases. EMBO J 18 339 352

29. Kozjak-PavlovicVDian-LothropEAMeineckeMKeppORossK 2009 Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis. PLoS Pathog 5 e1000629

30. BoyaPRoquesBKroemerG 2001 New EMBO members' review: viral and bacterial proteins regulating apoptosis at the mitochondrial level. Embo J 20 4325 4331

31. SavillJ 1998 Apoptosis. Phagocytic docking without shocking. Nature 392 442 443

32. ScaffidiPMisteliTBianchiME 2002 Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature 418 191 195

33. ShiYEvansJERockKL 2003 Molecular identification of a danger signal that alerts the immune system to dying cells. Nature 425 516 521

34. SavillJDransfieldIGregoryCHaslettC 2002 A blast from the past: clearance of apoptotic cells regulates immune responses. Nat Rev Immunol 2 965 975

35. FadokVABrattonDLKonowalAFreedPWWestcottJY 1998 Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF. J Clin Invest 101 890 898

36. CvetanovicMUckerDS 2004 Innate immune discrimination of apoptotic cells: repression of proinflammatory macrophage transcription is coupled directly to specific recognition. J Immunol 172 880 889

37. OrjihAUNussenzweigRS 1979 Plasmodium berghei: suppression of antibody response to sporozoite stage by acute blood stage infection. Clin Exp Immunol 38 1 8

38. OrengoJMWongKAOcana-MorgnerCRodriguezA 2008 A Plasmodium yoelii soluble factor inhibits the phenotypic maturation of dendritic cells. Malar J 7 254

39. GraeweSRetzlaffSStruckNJanseCJHeusslerVT 2009 Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites. Biotechnol J 4 895 902

40. GuntherKTummlerMArnoldHHRidleyRGomanM 1991 An exported protein of Plasmodium falciparum is synthesized as an integral membrane protein. Mol Biochem Parasitol 46 149 157

41. AurrecoecheaC 2009 PlasmoDB: a functional genomic database for malaria parasites. Nucleic Acids Res 37 D539 543

Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium
Článek Unconventional Repertoire Profile Is Imprinted during Acute Chikungunya Infection for Natural Killer Cells Polarization toward Cytotoxicity
Článek Envelope Deglycosylation Enhances Antigenicity of HIV-1 gp41 Epitopes for Both Broad Neutralizing Antibodies and Their Unmutated Ancestor Antibodies
Článek Co-opts GBF1 and CERT to Acquire Host Sphingomyelin for Distinct Roles during Intracellular Development
Článek Nrf2, a PPARγ Alternative Pathway to Promote CD36 Expression on Inflammatory Macrophages: Implication for Malaria
Článek Robust Antigen Specific Th17 T Cell Response to Group A Streptococcus Is Dependent on IL-6 and Intranasal Route of Infection
Článek Targeting of a Chlamydial Protease Impedes Intracellular Bacterial Growth
Článek The Protease Cruzain Mediates Immune Evasion
Článek High-Resolution Phenotypic Profiling Defines Genes Essential for Mycobacterial Growth and Cholesterol Catabolism
Článek Plague and Climate: Scales Matter
Článek Exhausted CD8 T Cells Downregulate the IL-18 Receptor and Become Unresponsive to Inflammatory Cytokines and Bacterial Co-infections
Článek Maturation-Induced Cloaking of Neutralization Epitopes on HIV-1 Particles
Článek Murine Gamma-herpesvirus Immortalization of Fetal Liver-Derived B Cells Requires both the Viral Cyclin D Homolog and Latency-Associated Nuclear Antigen
Článek Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
Článek A Trigger Enzyme in : Impact of the Glycerophosphodiesterase GlpQ on Virulence and Gene Expression
Článek Strain Specific Resistance to Murine Scrapie Associated with a Naturally Occurring Human Prion Protein Polymorphism at Residue 171
Článek Development of a Transformation System for : Restoration of Glycogen Biosynthesis by Acquisition of a Plasmid Shuttle Vector
Článek Monalysin, a Novel -Pore-Forming Toxin from the Pathogen Contributes to Host Intestinal Damage and Lethality
Článek Host Phylogeny Determines Viral Persistence and Replication in Novel Hosts
Článek BC2L-C Is a Super Lectin with Dual Specificity and Proinflammatory Activity
Článek Expression of the RAE-1 Family of Stimulatory NK-Cell Ligands Requires Activation of the PI3K Pathway during Viral Infection and Transformation
Článek Structure of the Vesicular Stomatitis Virus N-P Complex
Článek HSV Infection Induces Production of ROS, which Potentiate Signaling from Pattern Recognition Receptors: Role for S-glutathionylation of TRAF3 and 6
Článek The Human Papillomavirus E6 Oncogene Represses a Cell Adhesion Pathway and Disrupts Focal Adhesion through Degradation of TAp63β upon Transformation
Článek Analysis of Behavior and Trafficking of Dendritic Cells within the Brain during Toxoplasmic Encephalitis
Článek Exposure to the Viral By-Product dsRNA or Coxsackievirus B5 Triggers Pancreatic Beta Cell Apoptosis via a Bim / Mcl-1 Imbalance
Článek Multidrug Resistant 2009 A/H1N1 Influenza Clinical Isolate with a Neuraminidase I223R Mutation Retains Its Virulence and Transmissibility in Ferrets
Článek Structure of Herpes Simplex Virus Glycoprotein D Bound to the Human Receptor Nectin-1
Článek Step-Wise Loss of Bacterial Flagellar Torsion Confers Progressive Phagocytic Evasion
Článek Complex Recombination Patterns Arising during Geminivirus Coinfections Preserve and Demarcate Biologically Important Intra-Genome Interaction Networks
Článek An EGF-like Protein Forms a Complex with PfRh5 and Is Required for Invasion of Human Erythrocytes by
Článek Non-Lytic, Actin-Based Exit of Intracellular Parasites from Intestinal Cells
Článek The Fecal Viral Flora of Wild Rodents
Článek The General Transcriptional Repressor Tup1 Is Required for Dimorphism and Virulence in a Fungal Plant Pathogen
Článek Interferon Regulatory Factor-1 (IRF-1) Shapes Both Innate and CD8 T Cell Immune Responses against West Nile Virus Infection
Článek A Small Non-Coding RNA Facilitates Bacterial Invasion and Intracellular Replication by Modulating the Expression of Virulence Factors
Článek Evaluating the Sensitivity of to Biotin Deprivation Using Regulated Gene Expression
Článek The Motility of a Human Parasite, , Is Regulated by a Novel Lysine Methyltransferase
Článek Phosphodiesterase-4 Inhibition Alters Gene Expression and Improves Isoniazid – Mediated Clearance of in Rabbit Lungs
Článek Restoration of IFNγR Subunit Assembly, IFNγ Signaling and Parasite Clearance in Infected Macrophages: Role of Membrane Cholesterol
Článek Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole
Článek The Regulated Secretory Pathway in CD4 T cells Contributes to Human Immunodeficiency Virus Type-1 Cell-to-Cell Spread at the Virological Synapse
Článek Rerouting of Host Lipids by Bacteria: Are You CERTain You Need a Vesicle?
Článek Transmission Characteristics of the 2009 H1N1 Influenza Pandemic: Comparison of 8 Southern Hemisphere Countries
Článek Th2-polarised PrP-specific Transgenic T-cells Confer Partial Protection against Murine Scrapie
Článek Sequential Bottlenecks Drive Viral Evolution in Early Acute Hepatitis C Virus Infection
Článek Genomic Insights into the Origin of Parasitism in the Emerging Plant Pathogen
Článek Genomic and Proteomic Analyses of the Fungus Provide Insights into Nematode-Trap Formation
Článek Influenza Virus Ribonucleoprotein Complexes Gain Preferential Access to Cellular Export Machinery through Chromatin Targeting
Článek Alterations in the Transcriptome during Infection with West Nile, Dengue and Yellow Fever Viruses
Článek Protease-Sensitive Conformers in Broad Spectrum of Distinct PrP Structures in Sporadic Creutzfeldt-Jakob Disease Are Indicator of Progression Rate
Článek Vaccinia Virus Protein C6 Is a Virulence Factor that Binds TBK-1 Adaptor Proteins and Inhibits Activation of IRF3 and IRF7
Článek c-di-AMP Is a New Second Messenger in with a Role in Controlling Cell Size and Envelope Stress
Článek Structural and Functional Studies on the Interaction of GspC and GspD in the Type II Secretion System
Článek APOBEC3A Is a Specific Inhibitor of the Early Phases of HIV-1 Infection in Myeloid Cells
Článek Impairment of Immunoproteasome Function by β5i/LMP7 Subunit Deficiency Results in Severe Enterovirus Myocarditis
Článek HTLV-1 Propels Thymic Human T Cell Development in “Human Immune System” Rag2 gamma c Mice
Článek Tri6 Is a Global Transcription Regulator in the Phytopathogen
Článek Exploiting and Subverting Tor Signaling in the Pathogenesis of Fungi, Parasites, and Viruses
Článek The Next Opportunity in Anti-Malaria Drug Discovery: The Liver Stage
Článek Significant Effects of Antiretroviral Therapy on Global Gene Expression in Brain Tissues of Patients with HIV-1-Associated Neurocognitive Disorders
Článek Inhibition of Competence Development, Horizontal Gene Transfer and Virulence in by a Modified Competence Stimulating Peptide
Článek A Novel Metal Transporter Mediating Manganese Export (MntX) Regulates the Mn to Fe Intracellular Ratio and Virulence
Článek Rhoptry Kinase ROP16 Activates STAT3 and STAT6 Resulting in Cytokine Inhibition and Arginase-1-Dependent Growth Control
Článek Hsp90 Governs Dispersion and Drug Resistance of Fungal Biofilms
Článek Secretion of Genome-Free Hepatitis B Virus – Single Strand Blocking Model for Virion Morphogenesis of Para-retrovirus
Článek A Viral Ubiquitin Ligase Has Substrate Preferential SUMO Targeted Ubiquitin Ligase Activity that Counteracts Intrinsic Antiviral Defence
Článek Membrane Remodeling by the Double-Barrel Scaffolding Protein of Poxvirus
Článek A Diverse Population of Molecular Type VGIII in Southern Californian HIV/AIDS Patients
Článek Disruption of TLR3 Signaling Due to Cleavage of TRIF by the Hepatitis A Virus Protease-Polymerase Processing Intermediate, 3CD
Článek Quantitative Analyses Reveal Calcium-dependent Phosphorylation Sites and Identifies a Novel Component of the Invasion Motor Complex
Článek Discovery of the First Insect Nidovirus, a Missing Evolutionary Link in the Emergence of the Largest RNA Virus Genomes
Článek Old World Arenaviruses Enter the Host Cell via the Multivesicular Body and Depend on the Endosomal Sorting Complex Required for Transport
Článek Exploits a Unique Repertoire of Type IV Secretion System Components for Pilus Assembly at the Bacteria-Host Cell Interface
Článek Recurrent Signature Patterns in HIV-1 B Clade Envelope Glycoproteins Associated with either Early or Chronic Infections
Článok vyšiel v časopise

PLOS Pathogens

2011 Číslo 9
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

nový kurz
Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#