Bacteria-Induced Dscam Isoforms of the Crustacean,

English version České info

The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV) infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish.

Vyšlo v časopise: Bacteria-Induced Dscam Isoforms of the Crustacean,. PLoS Pathog 7(6): e32767. doi:10.1371/journal.ppat.1002062
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1002062

Souhrn

Zdroje

1. GarverLSXiZDimopoulosG 2008 Immunoglobulin superfamily members play an important role in the mosquito immune system. Dev Comp Immunol 32 519 531

2. DermodyTSKirchnerEGuglielmiKMStehleT 2009 Immunoglobulin superfamily virus receptors and the evolution of adaptive immunity. PLoS Pathog 5 e1000481

3. HutterHVogelBEPlenefischJDNorrisCRProencaRB 2000 Conservation and novelty in the evolution of cell adhesion and extracellular matrix genes. Science 287 989 994

4. WatsonFLPuttmann-HolgadoRThomasFLamarDLHughesM 2005 Extensive diversity of Ig-superfamily proteins in the immune system of insects. Science 309 1874 1878

5. ZhangSMAdemaCMKeplerTBLokerES 2004 Diversification of Ig superfamily genes in an invertebrate. Science 305 251 254

6. DongYTaylorHEDimopoulosG 2006 AgDscam, a hypervariable immunoglobulin domain-containing receptor of the Anopheles gambiae innate immune system. PLoS Biol 4 e229

7. YamakawaKHuotYKHaendeltMAHubertRChenXN 1998 DSCAM: a novel member of the immunoglobulin superfamily maps in a Down syndrome region and is involved in the development of the nervous system. Hum Mol Genet 7 227 237

8. AgarwalaKLGaneshSTsutsumiYSuzukiTAmanoK 2001 Cloning and functional characterization of DSCAML1, a novel DSCAM-like cell adhesion molecule that mediates homophilic intercellular adhesion. Biochem Biophys Res Commun 285 760 772

9. HattoriDMillardSSWojtowiczWMZipurskySL 2008 Dscam-mediated cell recognition regulates neural circuit formation. Annu Rev Cell Dev Biol 24 597 620

10. BoehmT 2007 Two in one: dual function of an invertebrate antigen receptor. Nat Immunol 8 1031 1033

11. SchmuckerDChenB 2009 Dscam and DSCAM: complex genes in simple animals, complex animals yet simple genes. Genes Dev 23 147 156

12. WojtowiczWMFlanaganJJMillardSSZipurskySLClemensJC 2004 Alternative splicing of Drosophila Dscam generates axon guidance receptors that exhibit isoform-specific homophilic binding. Cell 118 619 633

13. SchmuckerDClemensJCShuHWorbyCAXiaoJ 2000 Drosophila Dscam is an axon guidance receptor exhibiting extraordinary molecular diversity. Cell 101 671 684

14. StuartLMEzekowitzRA 2008 Phagocytosis and comparative innate immunity: learning on the fly. Nat Rev Immunol 8 131 141

15. WilliamsMJ 2007 Drosophila hemopoiesis and cellular immunity. J Immunol 178 4711 4716

16. BeveridgeTJ 1981 Ultrastructure, chemistry, and function of the bacterial wall. Int Rev Cytol 72 229 317

17. TakeuchiOHoshinoKKawaiTSanjoHTakadaH 1999 Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components. Immunity 11 443 451

18. ChouPHChangHSChenITLinHYChenYM 2009 The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail. Dev Comp Immunol 33 1258 1267

19. BartonESForrestJCConnollyJLChappellJDLiuY 2001 Junction adhesion molecule is a receptor for reovirus. Cell 104 441 451

20. ProtaAECampbellJASchellingPForrestJCWatsonMJ 2003 Crystal structure of human junctional adhesion molecule 1: implications for reovirus binding. Proc Natl Acad Sci U S A 100 5366 5371

21. WatthanasurorotAJiravanichpaisalPSöderhällISöderhällK 2010 A gC1qR prevents white spot syndrome virus replication in the freshwater crayfish Pacifastacus leniusculus. J Virol 84 10844 10851

22. GuindonSGascuelO 2003 A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52 696 704

23. HuelsenbeckJPRonquistF 2001 MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17 754 755

24. XieXLiHXuLYangF 2005 A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles. Virus Res 108 63 67

25. YuSLoweAW 2009 The pancreatic zymogen granule membrane protein, GP2, binds Escherichia coli Type 1 fimbriae. BMC Gastroenterol 9 58

26. HedJ 1986 Methods for distinguishing ingested from adhering particles. Methods Enzymol 132 198 204

27. SöderhällIBangyeekhunEMayoSSöderhällK 2003 Hemocyte production and maturation in an invertebrate animal; proliferation and gene expression in hematopoietic stem cells of Pacifastacus leniusculus. Dev Comp Immunol 27 661 672

28. SöderhällIKimYAJiravanichpaisalPLeeSYSöderhällK 2005 An ancient role for a prokineticin domain in invertebrate hematopoiesis. J Immunol 174 6153 6160

29. LiuHJiravanichpaisalPSöderhällICereniusLSöderhällK 2006 Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus. J Virol 80 10365 10371