Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA

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Nitrate serves as a major source of nitrogen nutrition for plants, algae and fungi, but the molecular details of how the nitrate signal is transduced to transcription factors regulating the expression of the nitrate assimilation genes are not known. To identify possible signaling mechanisms, we analyzed post-translational modifications in the pathway-specific activator NirA by mass spectrometry and found that NirA activity correlates with the oxidation status of a conserved methionine (Met169) in the regulatory nuclear export sequence (NES). This oxidation-reduction switch influences the overall conformation of the protein, which defines whether the NES is exposed or blocked. Site-directed mutagenesis and a forward-genetic suppressor screen identified two domains of NirA, which are regulating NES accessibility, subcellular distribution and the transcriptional activity of NirA. Our data for the first time establish a link between nitrate signaling and the redox status of the cell.

Vyšlo v časopise: Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA. PLoS Genet 11(7): e32767. doi:10.1371/journal.pgen.1005297
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005297

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