Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Mutant


Meiosis is a special cell division common in all sexually reproducing organisms. It consists of two successive rounds of chromosome segregation, preceded by a single DNA replication event. Homologous recombination is a key process that occurs during the first meiotic division. It guarantees the association of the homologous chromosomes by chiasmata, the cytological manifestations of reciprocal interchanges (crossovers, COs). The formation of COs during meiosis is fine-tuned by several mechanisms. One of them, reported in some model organisms, is CO homeostasis, which ensures a consistent number of COs despite variability in early recombination events. Here we described the analysis of Atfas1-4, a mutant defective for the histone chaperone CAF-1 (Chromatin Assembly Factor 1), which has an increase in double-strand breaks (DSBs), without a corresponding increase in COs. Interestingly, Atfas1-4 has a higher gene conversion (GC) frequency. These results demonstrate that Arabidopsis meiocytes are able to maintain WT levels of COs even when DSBs numbers are increased. Furthermore, we provide evidence for a prominent role for AtDMC1 in establishing interhomolog interactions in Arabidopsis.


Vyšlo v časopise: Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Mutant. PLoS Genet 11(7): e32767. doi:10.1371/journal.pgen.1005301
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pgen.1005301

Souhrn

Meiosis is a special cell division common in all sexually reproducing organisms. It consists of two successive rounds of chromosome segregation, preceded by a single DNA replication event. Homologous recombination is a key process that occurs during the first meiotic division. It guarantees the association of the homologous chromosomes by chiasmata, the cytological manifestations of reciprocal interchanges (crossovers, COs). The formation of COs during meiosis is fine-tuned by several mechanisms. One of them, reported in some model organisms, is CO homeostasis, which ensures a consistent number of COs despite variability in early recombination events. Here we described the analysis of Atfas1-4, a mutant defective for the histone chaperone CAF-1 (Chromatin Assembly Factor 1), which has an increase in double-strand breaks (DSBs), without a corresponding increase in COs. Interestingly, Atfas1-4 has a higher gene conversion (GC) frequency. These results demonstrate that Arabidopsis meiocytes are able to maintain WT levels of COs even when DSBs numbers are increased. Furthermore, we provide evidence for a prominent role for AtDMC1 in establishing interhomolog interactions in Arabidopsis.


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