Phosphorylation-Dependent Differential Regulation of Plant Growth,
Cell Death, and Innate Immunity by the Regulatory Receptor-Like Kinase
BAK1
Plants rely heavily on receptor-like kinases (RLKs) for perception and
integration of external and internal stimuli. The Arabidopsis regulatory
leucine-rich repeat RLK (LRR-RLK) BAK1 is involved in steroid hormone responses,
innate immunity, and cell death control. Here, we describe the differential
regulation of three different BAK1-dependent signaling pathways by a novel
allele of BAK1, bak1-5. Innate immune signaling mediated by the
BAK1-dependent RKs FLS2 and EFR is severely compromised in
bak1-5 mutant plants. However, bak1-5
mutants are not impaired in BR signaling or cell death control. We also show
that, in contrast to the RD kinase BRI1, the non-RD kinases FLS2 and EFR have
very low kinase activity, and we show that neither was able to
trans-phosphorylate BAK1 in vitro. Furthermore, kinase activity
for all partners is completely dispensable for the ligand-induced
heteromerization of FLS2 or EFR with BAK1 in planta, revealing
another pathway specific mechanistic difference. The specific suppression of
FLS2- and EFR-dependent signaling in bak1-5 is not due to a
differential interaction of BAK1-5 with the respective ligand-binding RK but
requires BAK1-5 kinase activity. Overall our results demonstrate a
phosphorylation-dependent differential control of plant growth, innate immunity,
and cell death by the regulatory RLK BAK1, which may reveal key differences in
the molecular mechanisms underlying the regulation of ligand-binding RD and
non-RD RKs.
Vyšlo v časopise:
Phosphorylation-Dependent Differential Regulation of Plant Growth,
Cell Death, and Innate Immunity by the Regulatory Receptor-Like Kinase
BAK1. PLoS Genet 7(4): e32767. doi:10.1371/journal.pgen.1002046
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pgen.1002046
Souhrn
Plants rely heavily on receptor-like kinases (RLKs) for perception and
integration of external and internal stimuli. The Arabidopsis regulatory
leucine-rich repeat RLK (LRR-RLK) BAK1 is involved in steroid hormone responses,
innate immunity, and cell death control. Here, we describe the differential
regulation of three different BAK1-dependent signaling pathways by a novel
allele of BAK1, bak1-5. Innate immune signaling mediated by the
BAK1-dependent RKs FLS2 and EFR is severely compromised in
bak1-5 mutant plants. However, bak1-5
mutants are not impaired in BR signaling or cell death control. We also show
that, in contrast to the RD kinase BRI1, the non-RD kinases FLS2 and EFR have
very low kinase activity, and we show that neither was able to
trans-phosphorylate BAK1 in vitro. Furthermore, kinase activity
for all partners is completely dispensable for the ligand-induced
heteromerization of FLS2 or EFR with BAK1 in planta, revealing
another pathway specific mechanistic difference. The specific suppression of
FLS2- and EFR-dependent signaling in bak1-5 is not due to a
differential interaction of BAK1-5 with the respective ligand-binding RK but
requires BAK1-5 kinase activity. Overall our results demonstrate a
phosphorylation-dependent differential control of plant growth, innate immunity,
and cell death by the regulatory RLK BAK1, which may reveal key differences in
the molecular mechanisms underlying the regulation of ligand-binding RD and
non-RD RKs.
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