MOS11: A New Component in the mRNA Export Pathway

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Nucleocytoplasmic trafficking is emerging as an important aspect of plant immunity. The three related pathways affecting plant immunity include Nuclear Localization Signal (NLS)–mediated nuclear protein import, Nuclear Export Signal (NES)–dependent nuclear protein export, and mRNA export relying on MOS3, a nucleoporin belonging to the Nup107–160 complex. Here we report the characterization, identification, and detailed analysis of Arabidopsis modifier of snc1, 11 (mos11). Mutations in MOS11 can partially suppress the dwarfism and enhanced disease resistance phenotypes of snc1, which carries a gain-of-function mutation in a TIR-NB-LRR type Resistance gene. MOS11 encodes a conserved eukaryotic protein with homology to the human RNA binding protein CIP29. Further functional analysis shows that MOS11 localizes to the nucleus and that the mos11 mutants accumulate more poly(A) mRNAs in the nucleus, likely resulting from reduced mRNA export activity. Epistasis analysis between mos3-1 and mos11-1 revealed that MOS11 probably functions in the same mRNA export pathway as MOS3, in a partially overlapping fashion, before the mRNA molecules pass through the nuclear pores. Taken together, MOS11 is identified as a new protein contributing to the transfer of mature mRNA from the nucleus to the cytosol.

Vyšlo v časopise: MOS11: A New Component in the mRNA Export Pathway. PLoS Genet 6(12): e32767. doi:10.1371/journal.pgen.1001250
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1001250

Souhrn

Zdroje

1. JonesJDG

DanglJL

2006 The plant immune system. Nature 444 323 329

2. Gimenez-IbanezS

HannDR

NtoukakisV

PetutschnigE

LipkaV

2009 AvrPtoB targets the LysM receptor kinase CERK1 to promote bacterial virulence on plants. Current Biology 19 423 429

3. GöhreV

SpallekT

HäwekerH

MersmannS

MentzelT

2008 Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB. Current Biology 18 1824 1832

4. ShanL

HeP

LiJ

HeeseA

PeckSC

2008 Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host & Microbe 4 17 27

5. XiangT

ZongN

ZouY

WuY

ZhangJ

2008 Pseudomonas syringae effector AvrPto blocks innate immunity by targeting receptor kinases. Current Biology 18 74 80

6. ChisholmST

CoakerG

DayB

StaskawiczBJ

2006 Host-microbe interactions: shaping the evolution of the plant immune response. Cell 124 803 814

7. LiX

ClarkeJD

ZhangY

DongX

2001 Activation of an EDS1-mediated R-gene pathway in the snc1 mutant leads to constitutive, NPR1-independent pathogen resistance. Molecular Plant-Microbe Interactions 14 1131 1139

8. ZhangY

GoritschnigS

DongX

LiX

2003 A gain-of-function mutation in a plant disease resistance gene leads to constitutive activation of downstream signal transduction pathways in suppressor of npr1-1, constitutive 1. Plant Cell 15 2636 2646

9. PalmaK

ZhangY

LiX

2005 An importin alpha homolog, MOS6, plays an important role in plant innate immunity. Current Biology 15 1129 1135

10. ChengYT

GermainH

WiermerM

BiD

XuF

2009 Nuclear pore complex component MOS7/Nup88 is required for innate immunity and nuclear accumulation of defense regulators in Arabidopsis. Plant Cell 21 2503 2516

11. ZhangY

LiX

2005 A putative nucleoporin 96 is required for both basal defense and constitutive resistance responses mediated by suppressor of npr1-1,constitutive 1. Plant Cell 17 1306 1316

12. ParryG

WardS

CernacA

DharmasiriS

EstelleM

2006 The Arabidopsis SUPPRESSOR OF AUXIN RESISTANCE proteins are nucleoporins with an important role in hormone signaling and development. Plant Cell 18 1590 1603

13. ZhuZ

XuF

ZhangY

ChengYT

WiermerM

2010 Arabidopsis resistance protein SNC1 activates immune responses through association with a transcriptional corepressor. Proceeding of the National Academy of Science, USA 107 13960 13965

14. GatfieldD

Le HirH

SchmittC

BraunIC

KocherT

2001 The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila. Current Biology 11 1716 1721

15. MeissnerM

LopatoS

GotzmannJ

SauermannG

BartaA

2003 Proto-oncoprotein TLS/FUS is associated to the nuclear matrix and complexed with splicing factors PTB, SRm160, and SR proteins. Experimental Cell Research 283 184 195

16. ZhouL

LuoMJ

StraesserK

KatahiraJ

HurtE

2000 The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. Nature 407 401 405

17. ZinsznerH

SokJ

ImmanuelD

YinY

RonD

1997 TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling. Journal of Cell Science 110 1741 1750

18. CherstvyAG

2009 Positively charged residues in DNA-binding domains of structural proteins follow sequence-specific positions of DNA phosphate groups. Journal of Physical Chemistry and Biology 113 4242 4247

19. HoribeK

MitakuS

2003 Positive charge cores in DNA-binding proteins. Genome Informatics 14 508 509

20. ShazmanS

Mandel-GutfreundY

2008 Classifying RNA-binding proteins based on electrostatic properties. PLoS Comput Biol 4 e1000146 doi:10.1371/journal.pcbi.1000146

21. Schulz-KnappeP

MägertHJ

DewaldB

MeyerM

CetinY

1996 HCC-1, a novel chemokine from human plasma. The Journal of Experimental Medicine 183 295 299

22. ForssmannU

MägertHJ

AdermannK

EscherSE

ForssmannWG

2001 Hemofiltrate CC chemokines with unique biochemical properties: HCC-1/CCL14a and HCC-2/CCL15. Journal of Leukocyte Biology 70 357 366

23. SugiuraT

SakuraiK

NaganoY

2007 Intracellular characterization of DDX39, a novel growth-associated RNA helicase. Experimental Cell Research 313 782 790

24. LeawCL

RenEC

ChoongML

2004 Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function. Cellular and Molecular Life Sciences 61 2264 2273

25. FukudaS

WuDW

StarkK

PelusLM

2002 Cloning and characterization of a proliferation-associated cytokine-inducible protein, CIP29. Biochemical and Biophysical Research Communications 292 593 600

26. DufuK

LivingstoneMJ

SeebacherJ

GygiSP

WilsonSA

2010 ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex. Genes and Development 24 2043 2053

27. Mosrin-HuamanC

HonorineR

RahmouniAR

2009 Expression of bacterial Rho factor in yeast identifies new factors involved in the functional interplay between transcription and mRNP biogenesis. Molecular and Cellular Biology 29 4033 4044

28. LuoMJ

ReedR

1999 Splicing is required for rapid and efficient mRNA export in metazoans. Proceedings of the National Academy of Sciences, USA 96 14937 14942

29. LemieuxC

BachandF

2009 Cotranscriptional recruitment of the nuclear poly(A)-binding protein Pab2 to nascent transcripts and association with translating mRNPs. Nucleic Acids Research 37 3418 3430

30. NapetschnigJ

KassubeSA

DeblerEW

WongRW

BlobelG

2009 Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19. Proceedings of the National Academy of Sciences, USA 106 3089 3094

31. IglesiasN

StutzF

2008 Regulation of mRNP dynamics along the export pathway. FEBS Letters 582 1987 1996

32. VinciguerraP

StutzF

2004 mRNA export: an assembly line from genes to nuclear pores. Current Opinion in Cell Biology 16 285 292

33. DongCH

HuX

TangW

ZhengX

KimYS

2006 A putative Arabidopsis nucleoporin, AtNUP160, is critical for RNA export and required for plant tolerance to cold stress. Molecular Cell Biology 26 9533 9543

34. JacobY

MongkolsiriwatanaC

VeleyKM

KimSY

MichaelsSD

2007 The nuclear pore protein AtTPR is required for RNA homeostasis, flowering time, and auxin signaling. Plant Physiology 144 1383 1390

35. JauvionV

ElmayanT

VaucheretH

2010 The conserved RNA trafficking proteins HPR1 and TEX1 are involved in the production of endogenous and exogenous small interfering RNA in Arabidopsis. Plant Cell 22 2697 2709

36. LuQ

TangX

TianG

WangF

LiuK

2010 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Plant Journal 61 259 270

37. YelinaNE

SmithLM

JonesAM

PatelK

KellyKA

2010 Putative Arabidopsis THO/TREX mRNA export complex is involved in transgene and endogenous siRNA biosynthesis. Proceeding of the National Academy of Science, USA 107 13948 13953

38. GongZ

DongC-H

LeeHS

ZhuJK

XiongL

2005 A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis. Plant Cell 17 256 267

39. MasudaS

DasR

ChengH

HurtE

DormanN

2005 Recruitment of the human TREX complex to mRNA during splicing. Genes and Development 19 1512 1517

40. RehwinkelJ

HeroldA

GariK

KocherT

RodeM

2004 Genome-wide analysis of mRNAs regulated by the THO complex in Drosophila melanogaster. Nature Structural Molecular Biology 11 558 566

41. LiX

ZhangY

ClarkeJD

LiY

DongX

1999 Identification and cloning of a negative regulator of systemic acquired resistance, SNI1, through a screen for suppressors of npr1-1. Cell 98 329 339

42. LiY

LiS

BiD

ChengYT

LiX

2010 SRFR1 negatively regulates plant NB-LRR resistance protein accumulation to prevent autoimmunity. PLoS Pathog 6 e1001111 doi:10.1371/journal.ppat.1001111

43. CloughSJ

BentAF

1998 Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16 735 743