Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery

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Rabies virus (RABV) is a neurotropic virus that depends on long distance axonal transport in order to reach the central nervous system (CNS). The strategy RABV uses to hijack the cellular transport machinery is unknown. Here we use live cell imaging to track RABV entry at nerve terminals and study its retrograde transport along the axon. First, we demonstrate that RABV interacts with the p75 neurotrophin receptor (p75NTR) at peripheral neuron tips to enter the axon. Then, characterizing RABV retrograde transport along the axon, we showed that the virus moves in acidic compartments, mostly with p75NTR. Interestingly, RABV is transported faster than NGF, an endogenous p75NTR ligand. Finally, we determine that p75NTR-dependent transport of RABV is faster and more directed than p75NTR-independent RABV transport. Hence, RABV not only exploits the neurotrophin transport machinery, but also has a positive influence on transport kinetics, thus facilitating its own arrival at the CNS.

Vyšlo v časopise: Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery. PLoS Pathog 10(8): e32767. doi:10.1371/journal.ppat.1004348
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004348

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