Bringing Culture to the Uncultured: and Lessons for Obligate Intracellular Bacterial Pathogens

1. VothDE, HeinzenRA (2007) Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii. Cell Microbiol 9 : 829–840.

2. EnserinkM (2010) Questions abound in Q-fever explosion in the Netherlands. Science 327 : 266–267.

3. BurtonPR, StueckemannJ, WelshRM, ParetskyD (1978) Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells. Infect Immun 21 : 556–566.

4. HackstadtT, WilliamsJC (1981) Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii. Proc Natl Acad Sci USA 78 : 3240–3244.

5. HackstadtT, WilliamsJC (1983) pH dependence of the Coxiella burnetii glutamate transport system. J Bacteriol 154 : 598–603.

6. HoweD, ShannonJG, WinfreeS, DorwardDW, HeinzenRA (2010) Coxiella burnetii phase I and II variants replicate with similar kinetics in degradative phagolysosome-like compartments of human macrophages. Infect Immun 78 : 3465–3474.

7. AmanoK, WilliamsJC (1984) Sensitivity of Coxiella burnetii peptidoglycan to lysozyme hydrolysis and correlation of sacculus rigidity with peptidoglycan-associated proteins. J Bacteriol 160 : 989–993.

8. FieldsKA, HackstadtT (2002) The chlamydial inclusion: escape from the endocytic pathway. Annu Rev Cell Dev Biol 18 : 221–245.

9. GrieshaberS, SwansonJA, HackstadtT (2002) Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes. Cell Microbiol 4 : 273–283.

10. SakaHA, ValdiviaRH (2010) Acquisition of nutrients by Chlamydiae: unique challenges of living in an intracellular compartment. Curr Opin Microbiol 13 : 4–10.

11. OmslandA, SagerJ, NairV, SturdevantDE, HackstadtT (2012) Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium. Proc Natl Acad Sci USA 109 : 19781–19785.

12. NiuH, XiongQ, YamamotoA, Hayashi-NishinoM, RikihisaY (2012) Autophagosomes induced by a bacterial Beclin 1 binding protein facilitate obligatory intracellular infection. Proc Natl Acad Sci USA 109 : 20800–20807.

13. BarnewallRE, RikihisaY, LeeEH (1997) Ehrlichia chaffeensis inclusions are early endosomes which selectively accumulate transferrin receptor. Infect Immun 65 : 1455–1461.

14. AudiaJP, WinklerHH (2006) Study of the five Rickettsia prowazekii proteins annotated as ATP/ADP translocases (Tlc): Only Tlc1 transports ATP/ADP, while Tlc4 and Tlc5 transport other ribonucleotides. J Bacteriol 188 : 6261–6268.

15. FuchsTM, EisenreichW, HeesemannJ, GoebelW (2012) Metabolic adaptation of human pathogenic and related nonpathogenic bacteria to extra -⁠ and intracellular habitats. FEMS Microbiol Rev 36 : 435–462.

16. ChenDE, PodellS, SauerJD, SwansonMS, SaierMHJr (2008) The phagosomal nutrient transporter (Pht) family. Microbiology 154 : 42–53.

17. SauerJD, BachmanMA, SwansonMS (2005) The phagosomal transporter A couples threonine acquisition to differentiation and replication of Legionella pneumophila in macrophages. Proc Natl Acad Sci USA 102 : 9924–9929.

18. BearePA, UnsworthN, AndohM, VothDE, OmslandA, et al. (2009) Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella. Infect Immun 77 : 642–656.

19. TjadenJ, WinklerHH, SchwoppeC, Van Der LaanM, MohlmannT, et al. (1999) Two nucleotide transport proteins in Chlamydia trachomatis, one for net nucleoside triphosphate uptake and the other for transport of energy. J Bacteriol 181 : 1196–1202.

20. MinCK, YangJS, KimS, ChoiMS, KimIS, et al. (2008) Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order. Comp Funct Genomics 623145.

21. DmitrievRI, ZhdanovAV, JasionekG, PapkovskyDB (2012) Assessment of cellular oxygen gradients with a panel of phosphorescent oxygen-sensitive probes. Anal Chem 84 : 2930–2938.

22. JuulN, JensenH, HvidM, ChristiansenG, BirkelundS (2007) Characterization of in vitro chlamydial cultures in low-oxygen atmospheres. J Bacteriol 189 : 6723–6726.

23. OmslandA, CockrellDC, FischerER, HeinzenRA (2008) Sustained axenic metabolic activity by the obligate intracellular bacterium Coxiella burnetii. J Bacteriol 190 : 3203–3212.

24. OmslandA, CockrellDC, HoweD, FischerER, VirtanevaK, et al. (2009) Host cell-free growth of the Q fever bacterium Coxiella burnetii. Proc Natl Acad Sci USA 106 : 4430–4434.

25. HaiderS, WagnerM, SchmidMC, SixtBS, ChristianJG, et al. (2010) Raman microspectroscopy reveals long-term extracellular activity of chlamydiae. Mol Microbiol 77 : 687–700.

26. MolofskyAB, SwansonMS (2004) Differentiate to thrive: lessons from the Legionella pneumophila life cycle. Mol Microbiol 53 : 29–40.

27. ColemanSA, FischerER, HoweD, MeadDJ, HeinzenRA (2004) Temporal analysis of Coxiella burnetii morphological differentiation. J Bacteriol 186 : 7344–7352.

28. StewartEJ (2012) Growing unculturable bacteria. J Bacteriol 194 : 4151–4160.

29. SizovaMV, HohmannT, HazenA, PasterBJ, HalemSR, et al. (2012) New approaches for isolation of previously uncultivated oral bacteria. Appl Environ Microbiol 78 : 194–203.

30. SilvaMT (2012) Classical labeling of bacterial pathogens according to their lifestyle in the host: inconsistencies and alternatives. Front Microbiol 3 : 71.

31. BearePA, LarsonCL, GilkSD, HeinzenRA (2012) Two systems for targeted gene deletion in Coxiella burnetii. Appl Environ Microbiol 78 : 4580–4589.