-Mediated Detoxification of Reactive Oxygen
Species Is Required for Full Virulence in the Rice Blast Fungus
During plant-pathogen interactions, the plant may mount several types of defense
responses to either block the pathogen completely or ameliorate the amount of
disease. Such responses include release of reactive oxygen species (ROS) to
attack the pathogen, as well as formation of cell wall appositions (CWAs) to
physically block pathogen penetration. A successful pathogen will likely have
its own ROS detoxification mechanisms to cope with this inhospitable
environment. Here, we report one such candidate mechanism in the rice blast
fungus, Magnaporthe oryzae, governed by a gene we refer to as
MoHYR1. This gene (MGG_07460) encodes a glutathione
peroxidase (GSHPx) domain, and its homologue in yeast was reported to
specifically detoxify phospholipid peroxides. To characterize this gene in
M. oryzae, we generated a deletion
mutantΔhyr1 which showed growth inhibition with
increased amounts of hydrogen peroxide (H2O2). Moreover,
we observed that the fungal mutants had a decreased ability to tolerate ROS
generated by a susceptible plant, including ROS found associated with CWAs.
Ultimately, this resulted in significantly smaller lesion sizes on both barley
and rice. In order to determine how this gene interacts with other (ROS)
scavenging-related genes in M. oryzae, we compared expression
levels of ten genes in mutant versus wild type with and without
H2O2. Our results indicated that the
HYR1 gene was important for allowing the fungus to tolerate
H2O2
in vitro and in planta and that this ability
was directly related to fungal virulence.
Vyšlo v časopise:
-Mediated Detoxification of Reactive Oxygen
Species Is Required for Full Virulence in the Rice Blast Fungus. PLoS Pathog 7(4): e32767. doi:10.1371/journal.ppat.1001335
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1001335
Souhrn
During plant-pathogen interactions, the plant may mount several types of defense
responses to either block the pathogen completely or ameliorate the amount of
disease. Such responses include release of reactive oxygen species (ROS) to
attack the pathogen, as well as formation of cell wall appositions (CWAs) to
physically block pathogen penetration. A successful pathogen will likely have
its own ROS detoxification mechanisms to cope with this inhospitable
environment. Here, we report one such candidate mechanism in the rice blast
fungus, Magnaporthe oryzae, governed by a gene we refer to as
MoHYR1. This gene (MGG_07460) encodes a glutathione
peroxidase (GSHPx) domain, and its homologue in yeast was reported to
specifically detoxify phospholipid peroxides. To characterize this gene in
M. oryzae, we generated a deletion
mutantΔhyr1 which showed growth inhibition with
increased amounts of hydrogen peroxide (H2O2). Moreover,
we observed that the fungal mutants had a decreased ability to tolerate ROS
generated by a susceptible plant, including ROS found associated with CWAs.
Ultimately, this resulted in significantly smaller lesion sizes on both barley
and rice. In order to determine how this gene interacts with other (ROS)
scavenging-related genes in M. oryzae, we compared expression
levels of ten genes in mutant versus wild type with and without
H2O2. Our results indicated that the
HYR1 gene was important for allowing the fungus to tolerate
H2O2
in vitro and in planta and that this ability
was directly related to fungal virulence.
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