is an Unstable Pathogen
Showing Evidence of Significant Genomic Flux
Citrobacter rodentium is a natural mouse pathogen that causes
attaching and effacing (A/E) lesions. It shares a common virulence strategy with
the clinically significant human A/E pathogens enteropathogenic E.
coli (EPEC) and enterohaemorrhagic E. coli (EHEC)
and is widely used to model this route of pathogenesis. We previously reported
the complete genome sequence of C. rodentium ICC168, where we
found that the genome displayed many characteristics of a newly evolved
pathogen. In this study, through PFGE, sequencing of isolates showing variation,
whole genome transcriptome analysis and examination of the mobile genetic
elements, we found that, consistent with our previous hypothesis, the genome of
C. rodentium is unstable as a result of repeat-mediated,
large-scale genome recombination and because of active transposition of mobile
genetic elements such as the prophages. We sequenced an additional C.
rodentium strain, EX-33, to reveal that the reference strain ICC168
is representative of the species and that most of the inactivating mutations
were common to both isolates and likely to have occurred early on in the
evolution of this pathogen. We draw parallels with the evolution of other
bacterial pathogens and conclude that C. rodentium is a
recently evolved pathogen that may have emerged alongside the development of
inbred mice as a model for human disease.
Vyšlo v časopise:
is an Unstable Pathogen
Showing Evidence of Significant Genomic Flux. PLoS Pathog 7(4): e32767. doi:10.1371/journal.ppat.1002018
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.ppat.1002018
Souhrn
Citrobacter rodentium is a natural mouse pathogen that causes
attaching and effacing (A/E) lesions. It shares a common virulence strategy with
the clinically significant human A/E pathogens enteropathogenic E.
coli (EPEC) and enterohaemorrhagic E. coli (EHEC)
and is widely used to model this route of pathogenesis. We previously reported
the complete genome sequence of C. rodentium ICC168, where we
found that the genome displayed many characteristics of a newly evolved
pathogen. In this study, through PFGE, sequencing of isolates showing variation,
whole genome transcriptome analysis and examination of the mobile genetic
elements, we found that, consistent with our previous hypothesis, the genome of
C. rodentium is unstable as a result of repeat-mediated,
large-scale genome recombination and because of active transposition of mobile
genetic elements such as the prophages. We sequenced an additional C.
rodentium strain, EX-33, to reveal that the reference strain ICC168
is representative of the species and that most of the inactivating mutations
were common to both isolates and likely to have occurred early on in the
evolution of this pathogen. We draw parallels with the evolution of other
bacterial pathogens and conclude that C. rodentium is a
recently evolved pathogen that may have emerged alongside the development of
inbred mice as a model for human disease.
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