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Proteolysis of Virulence Regulator ToxR Is Associated with Entry of into a Dormant State
Non-obligate bacterial pathogens must alter their gene expression profiles when transitioning between environments. Vibrio cholerae is a natural inhabitant of aquatic ecosystems and the etiological agent of the severe diarrheal disease, cholera. Its virulence gene regulation is controlled by a complex transcriptional cascade involving a membrane-localized regulator termed ToxR. Here we show that ToxR undergoes proteolysis under nutrient limitation at alkaline pH and this loss is associated with the entry of V. cholerae into a dormant state, similar to that found in its natural environment between epidemics. Thus, to our knowledge, we provide the first evidence of a link between the proteolysis of a virulence regulator and the entry of a bacterial pathogen into an environmentally persistent state.
Vyšlo v časopise: Proteolysis of Virulence Regulator ToxR Is Associated with Entry of into a Dormant State. PLoS Genet 11(4): e32767. doi:10.1371/journal.pgen.1005145
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005145Souhrn
Non-obligate bacterial pathogens must alter their gene expression profiles when transitioning between environments. Vibrio cholerae is a natural inhabitant of aquatic ecosystems and the etiological agent of the severe diarrheal disease, cholera. Its virulence gene regulation is controlled by a complex transcriptional cascade involving a membrane-localized regulator termed ToxR. Here we show that ToxR undergoes proteolysis under nutrient limitation at alkaline pH and this loss is associated with the entry of V. cholerae into a dormant state, similar to that found in its natural environment between epidemics. Thus, to our knowledge, we provide the first evidence of a link between the proteolysis of a virulence regulator and the entry of a bacterial pathogen into an environmentally persistent state.
Zdroje
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