1. UNAIDS (2012) http://www.unaids.org/en/media/unaids/contentassets/documents/epidemiology/2012/gr2012/20121120_UNAIDS_Global_Report_2012_with_annexes_en.pdf.
2. HaaseAT (2011) Early events in sexual transmission of HIV and SIV and opportunities for interventions. Annu Rev Med 62: 127–139.
3. BrenchleyJM, DouekDC (2008) HIV infection and the gastrointestinal immune system. Mucosal Immunol 1: 23–30.
4. DouekDC, RoedererM, KoupRA (2009) Emerging concepts in the immunopathogenesis of AIDS. Annu Rev Med 60: 471–484.
5. VeazeyRS, DeMariaM, ChalifouxLV, ShvetzDE, PauleyDR, et al. (1998) Gastrointestinal tract as a major site of CD4+ T cell depletion and viral replication in SIV infection. Science 280: 427–431.
6. BrenchleyJM, SchackerTW, RuffLE, PriceDA, TaylorJH, et al. (2004) CD4+ T cell depletion during all stages of HIV disease occurs predominantly in the gastrointestinal tract. J Exp Med 200: 749–759.
7. LacknerAA, LedermanMM, RodriguezB (2012) HIV Pathogenesis: The Host. Cold Spring Harb Perspect Med 2: a007005.
8. HaaseAT (2005) Perils at mucosal front lines for HIV and SIV and their hosts. Nat Rev Immunol 5: 783–792.
9. Guy-GrandD, VassalliP (1993) Gut intraepithelial T lymphocytes. Curr Opin Immunol 5: 247–252.
10. DouekD (2007) HIV disease progression: immune activation, microbes, and a leaky gut. Top HIV Med 15: 114–117.
11. BrenchleyJM, PriceDA, SchackerTW, AsherTE, SilvestriG, et al. (2006) Microbial translocation is a cause of systemic immune activation in chronic HIV infection. Nat Med 12: 1365–1371.
12. DentonPW, GarciaJV (2012) Mucosal HIV-1 transmission and prevention strategies in BLT humanized mice. Trends in microbiology 20: 268–274.
13. BrainardDM, SeungE, FrahmN, CariappaA, BaileyCC, et al. (2009) Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol 83: 7305–7321.
14. DentonPW, GarciaJV (2011) Humanized mouse models of HIV infection. AIDS Rev 13: 135–148.
15. DentonPW, EstesJD, SunZ, OthienoFA, WeiBL, et al. (2008) Antiretroviral pre-exposure prophylaxis prevents vaginal transmission of HIV-1 in humanized BLT mice. PLoS Med 5: e16.
16. SunZ, DentonPW, EstesJD, OthienoFA, WeiBL, et al. (2007) Intrarectal transmission, systemic infection, and CD4+ T cell depletion in humanized mice infected with HIV-1. J Exp Med 204: 705–714.
17. LanP, TonomuraN, ShimizuA, WangS, YangYG (2006) Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus/liver and CD34+ cell transplantation. Blood 108: 487–492.
18. RajeshD, ZhouY, Jankowska-GanE, RoenneburgDA, DartML, et al. (2010) Th1 and Th17 immunocompetence in humanized NOD/SCID/IL2rgammanull mice. Human immunology 71: 551–559.
19. Barre-SinoussiF, ChermannJC, ReyF, NugeyreMT, ChamaretS, et al. (1983) Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 220: 868–871.
20. GalloRC, SarinPS, GelmannEP, Robert-GuroffM, RichardsonE, et al. (1983) Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS). Science 220: 865–867.
21. OrensteinJM (2007) Replication of HIV-1 in vivo and in vitro. Ultrastruct Pathol 31: 151–167.
22. BriggsJA, RichesJD, GlassB, BartonovaV, ZanettiG, et al. (2009) Structure and assembly of immature HIV. Proc Natl Acad Sci U S A 106: 11090–11095.
23. BenjaminJ, Ganser-PornillosBK, TivolWF, SundquistWI, JensenGJ (2005) Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography. J Mol Biol 346: 577–588.
24. WrightER, SchoolerJB, DingHJ, KiefferC, FillmoreC, et al. (2007) Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. EMBO J 26: 2218–2226.
25. CarlsonLA, de MarcoA, OberwinklerH, HabermannA, BriggsJA, et al. (2010) Cryo electron tomography of native HIV-1 budding sites. PLoS Pathog 6: e1001173.
26. CarlsonLA, BriggsJA, GlassB, RichesJD, SimonMN, et al. (2008) Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis. Cell Host Microbe 4: 592–599.
27. FeltsRL, NarayanK, EstesJD, ShiD, TrubeyCM, et al. (2010) 3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells. Proc Natl Acad Sci U S A 107: 13336–13341.
28. SougratR, BartesaghiA, LifsonJD, BennettAE, BessJW, et al. (2007) Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entry. PLoS Pathog 3: e63.
29. WalkerMR, PatelKK, StappenbeckTS (2009) The stem cell niche. J Pathol 217: 169–180.
30. McIntoshJR, NicastroD, MastronardeDN (2005) New views of cells in 3D: an introduction to electron tomography. Trends Cell Biol 15: 43–51.
31. GilkeyJC, StaehelinLA (1986) Advances in Ultrarapid Freezing for the Preservation of Cellular Ultrastructure. J Elect Microsc Tech 3: 177–210.
32. MorphewM, HeW, BjorkmanPJ, McIntoshJR (2008) Silver enhancement of Nanogold particles during freeze substitution for electron microscopy. J Microsc 230: 263–267.
33. LadinskyMS, HowellKE (2007) Electron tomography of immunolabeled cryosections. Methods Cell Biol 79: 543–558.
34. LutherPK, LawrenceMC, CrowtherRA (1988) A method for monitoring the collapse of plastic sections as a function of electron dose. Ultramicroscopy 24: 7–18.
35. FullerSD, WilkT, GowenBE, KrausslichHG, VogtVM (1997) Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle. Curr Biol 7: 729–738.
36. WilkT, GrossI, GowenBE, RuttenT, de HaasF, et al. (2001) Organization of immature human immunodeficiency virus type 1. J Virol 75: 759–771.
37. GanserBK, LiS, KlishkoVY, FinchJT, SundquistWI (1999) Assembly and analysis of conical models for the HIV-1 core. Science 283: 80–83.
38. HockleyDJ, WoodRD, JacobsJP, GarrettAJ (1988) Electron microscopy of human immunodeficiency virus. J Gen Virol 69: 2455–2469.
39. ZhuP, LiuJ, BessJJr, ChertovaE, LifsonJD, et al. (2006) Distribution and three-dimensional structure of AIDS virus envelope spikes. Nature 441: 847–852.
40. ChertovaE, Bess JrJWJr, CriseBJ, SowderIR, SchadenTM, et al. (2002) Envelope glycoprotein incorporation, not shedding of surface envelope glycoprotein (gp120/SU), Is the primary determinant of SU content of purified human immunodeficiency virus type 1 and simian immunodeficiency virus. J Virol 76: 5315–5325.
41. MagadanJG, Perez-VictoriaFJ, SougratR, YeY, StrebelK, et al. (2010) Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps. PLoS Pathog 6: e1000869.
42. BriggsJA, SimonMN, GrossI, KrausslichHG, FullerSD, et al. (2004) The stoichiometry of Gag protein in HIV-1. Nat Struct Mol Biol 11: 672–675.
43. DenekaM, Pelchen-MatthewsA, BylandR, Ruiz-MateosE, MarshM (2007) In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. J Cell Biol 177: 329–341.
44. BennettAE, NarayanK, ShiD, HartnellLM, GoussetK, et al. (2009) Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. PLoS Pathog 5: e1000591.
45. Pelchen-MatthewsA, KramerB, MarshM (2003) Infectious HIV-1 assembles in late endosomes in primary macrophages. J Cell Biol 162: 443–455.
46. RaposoG, MooreM, InnesD, LeijendekkerR, Leigh-BrownA, et al. (2002) Human macrophages accumulate HIV-1 particles in MHC II compartments. Traffic 3: 718–729.
47. JollyC, KashefiK, HollinsheadM, SattentauQJ (2004) HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse. J Exp Med 199: 283–293.
48. HioeCE, ChienPCJr, LuC, SpringerTA, WangXH, et al. (2001) LFA-1 expression on target cells promotes human immunodeficiency virus type 1 infection and transmission. J Virol 75: 1077–1082.
49. RizzutoCD, SodroskiJG (1997) Contribution of virion ICAM-1 to human immunodeficiency virus infectivity and sensitivity to neutralization. J Virol 71: 4847–4851.
50. RobertsonDL, AndersonJP, BradacJA, CarrJK, FoleyB, et al. (2000) HIV-1 nomenclature proposal. Science 288: 55–56.
51. AggarwalA, IemmaTL, ShihI, NewsomeTP, McAlleryS, et al. (2012) Mobilization of HIV spread by diaphanous 2 dependent filopodia in infected dendritic cells. PLoS Pathog 8: e1002762.
52. SundquistWI, KrausslichHG (2012) HIV-1 Assembly, Budding, and Maturation. Cold Spring Harb Perspect Med 2: a006924.
53. VottelerJ, IavnilovitchE, FingrutO, ShemeshV, TaglichtD, et al. (2009) Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release. BMC Biochem 10: 12.
54. CarlsonLA, HurleyJH (2012) In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Proc Natl Acad Sci U S A 109: 16928–16933.
55. BouraE, RozyckiB, ChungHS, HerrickDZ, CanagarajahB, et al. (2012) Solution structure of the ESCRT-I and -II supercomplex: implications for membrane budding and scission. Structure 20: 874–886.
56. LataS, SchoehnG, JainA, PiresR, PiehlerJ, et al. (2008) Helical structures of ESCRT-III are disassembled by VPS4. Science 321: 1354–1357.
57. HansonPI, RothR, LinY, HeuserJE (2008) Plasma membrane deformation by circular arrays of ESCRT-III protein filaments. J Cell Biol 180: 389–402.
58. von SchwedlerUK, StuchellM, MullerB, WardDM, ChungHY, et al. (2003) The protein network of HIV budding. Cell 114: 701–713.
59. BaumgartelV, IvanchenkoS, DupontA, SergeevM, WisemanPW, et al. (2011) Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component. Nat Cell Biol 13: 469–474.
60. JouvenetN, ZhadinaM, BieniaszPD, SimonSM (2011) Dynamics of ESCRT protein recruitment during retroviral assembly. Nat Cell Biol 13: 394–401.
61. YuZ, GonciarzMD, SundquistWI, HillCP, JensenGJ (2008) Cryo-EM structure of dodecameric Vps4p and its 2∶1 complex with Vta1p. J Mol Biol 377: 364–377.
62. AntonPA, MitsuyasuRT, DeeksSG, ScaddenDT, WagnerB, et al. (2003) Multiple measures of HIV burden in blood and tissue are correlated with each other but not with clinical parameters in aviremic subjects. AIDS 17: 53–63.
63. ChunTW, CarruthL, FinziD, ShenX, DiGiuseppeJA, et al. (1997) Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature 387: 183–188.
64. WelschS, KepplerOT, HabermannA, AllespachI, Krijnse-LockerJ, et al. (2007) HIV-1 buds predominantly at the plasma membrane of primary human macrophages. PLoS Pathog 3: e36.
65. ChuH, WangJJ, QiM, YoonJJ, WenX, et al. (2012) The intracellular virus-containing compartments in primary human macrophages are largely inaccessible to antibodies and small molecules. PLoS One 7: e35297.
66. de MarcoA, MullerB, GlassB, RichesJD, KrausslichHG, et al. (2010) Structural analysis of HIV-1 maturation using cryo-electron tomography. PLoS Pathog 6: e1001215.
67. SwartzMA, FleuryME (2007) Interstitial flow and its effects in soft tissues. Annu Rev Biomed Eng 9: 229–256.
68. WelschS, HabermannA, JagerS, MullerB, Krijnse-LockerJ, et al. (2006) Ultrastructural analysis of ESCRT proteins suggests a role for endosome-associated tubular-vesicular membranes in ESCRT function. Traffic 7: 1551–1566.
69. BoutwellCL, RowleyCF, EssexM (2009) Reduced viral replication capacity of human immunodeficiency virus type 1 subtype C caused by cytotoxic-T-lymphocyte escape mutations in HLA-B57 epitopes of capsid protein. J Virol 83: 2460–2468.
70. MastronardeDN (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152: 36–51.
71. MastronardeDN (2008) Correction for non-perpendicularity of beam and tilt axis in tomographic reconstructions with the IMOD package. J Microsc 230: 212–217.
72. LadinskyMS, WuCC, McIntoshS, McIntoshJR, HowellKE (2002) Structure of the Golgi and distribution of reporter molecules at 20 degrees C reveals the complexity of the exit compartments. Mol Biol Cell 13: 2810–2825.