The formation of haploid gametes from diploid germ cells requires a specialized reductive cell division known as meiosis. This reductive division is enabled by chromosomal events that occur during meiotic prophase, including synapsis and crossing-over of homologous chromosomes. These chromosomal events involve meiosis-specific genes that must be expressed before they act during meiosis. Using gene expression profiling, we identified a set of mammalian meiosis-specific genes. To understand how expression of these genes is controlled, we examined their expression in the absence of known regulators of the chromosomal events: 1) retinoic acid (RA), which induces meiosis, 2) Dazl, which is required for germ cell competence to respond to RA, and 3) Stra8, which is induced by RA and is required for the chromosomal events of meiotic prophase. We uncover two key features of gene regulation. First, while the genes require RA and Dazl to be expressed, they vary in their dependence on Stra8, thus creating a regulatory hierarchy. Genes induced independently of Stra8, and thus early in this hierarchy, may encode proteins that are stockpiled in anticipation of the chromosomal events. Second, Stra8 induces its own down-regulation, and may thus prevent repeated induction of meiosis in a single germ cell.
Vyšlo v časopise: A Gene Regulatory Program for Meiotic Prophase in the Fetal Ovary. PLoS Genet 11(9): e32767. doi:10.1371/journal.pgen.1005531 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005531
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