Pluripotency of ES cells derived from tetraploid embryo complemented male mice

English version České info

Abstract:
Pluripotent mouse embryonic stem (ES) cells have the ability to generate completely ES cell-derived mice by tetraploid (TE-4N) complementation although; derivation of ES cells from TE-4N animals has not been well defined. In this study, we generated TE-4N mice from high passage number ES cells and, in turn, determined the possibility of deriving ES cells from the TE-4N mice. The results showed that adult fertile TE-4N mice could be generated by aggregation of tetraploid embryos using ES cells passaged 23 times. Furthermore, ES cells could be derived from the TE-4N male mice and as judged by molecular characterisation and a study of chimeras, these ES cells were pluripotent. The findings demonstrate that tetraploid complementation is an efficient way to produce fertile mice, which can then give rise to pluripotent ES cells.

Keywords:
embryonic stem cells; pluripotency; tetraploid embryos

Autoři: Shuyu Li ^1,2; Baojiang Wu ²; Hongyan Xue ²; Lixia Zhao ²; Jie Su ²; Yunxia Li ¹; Wei Sun ²; Shuxiang Hu ²; Yao Li ¹; Siqin Bao ¹; Yanfeng Dai ¹; Jitong Guo ²; Xihe Li ^1,2*
Působiště autorů: Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University, Hohhot, P. R. China ¹; Inner Mongolia Saikexing Reproductive Biotechnology Co. Ltd., Hohhot, P. R. China ²
Vyšlo v časopise: Cell Biology International Reports, 21, 2014, č. 2, s. 71-76
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1002/cbi3.10019

© 2014 The Authors. Cell Biology International Reports published by John Wiley & Sons Ltd on behalf of the International Federation for Cell Biology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Cell Biol Int Rep 21 (2014) 17–24 2014 The Authors. Cell Biology International Reports published by John Wiley & Sons Ltd on behalf of the International Federation for Cell Biology.© 2014 The Authors. Cell Biology International Reports published by John Wiley & Sons Ltd on behalf of the International Federation for Cell Biology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Cell Biol Int Rep 21 (2014) 17–24 2014 The Authors. Cell Biology International Reports published by John Wiley & Sons Ltd on behalf of the International Federation for Cell Biology.

Souhrn

Keywords:
embryonic stem cells; pluripotency; tetraploid embryos

Zdroje

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