Transcriptional Control of an Essential Ribozyme in Reveals an Ancient Evolutionary Divide in Animals

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The processing of the 5′ end of nascent tRNAs is catalyzed by ribonuclease P (RNase P), an essential enzyme. In the ribonucleoprotein form of this enzyme, the RNase P RNA (RPR) functions as a ribozyme aided by protein cofactors. All previously examined eukaryotic RPR genes are transcribed from their own promoters by RNA pol III. In contrast, the Drosophila RPR gene is embedded in an intron of a recipient gene. We have shown that the embedded sequence, the only copy of RPR in the genome, is transcribed by pol II from the promoter of its recipient gene and encodes the functional RPR. Analysis of other animal genomes revealed that an embedded RPR is also present in the genomes of other insects and crustaceans. This feature provides evidence that the mode of transcription of RPR changed as the result of insertion into a recipient gene approximately 500 million years ago. This new, inserted type of RPR must first have appeared in the arthropod lineage in a common ancestor of insects and crustaceans.

Vyšlo v časopise: Transcriptional Control of an Essential Ribozyme in Reveals an Ancient Evolutionary Divide in Animals. PLoS Genet 11(1): e32767. doi:10.1371/journal.pgen.1004893
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004893

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