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Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57


The herpesvirus proteins HSV-1 ICP27 and HVS ORF57 promote viral mRNA export by utilizing the cellular mRNA export machinery. This function is triggered by binding to proteins of the transcription-export (TREX) complex, in particular to REF/Aly which directs viral mRNA to the TAP/NFX1 pathway and, subsequently, to the nuclear pore for export to the cytoplasm. Here we have determined the structure of the REF-ICP27 interaction interface at atomic-resolution and provided a detailed comparison of the binding interfaces between ICP27, ORF57 and REF using solution-state NMR. Despite the absence of any obvious sequence similarity, both viral proteins bind on the same site of the folded RRM domain of REF, via short but specific recognition sites. The regions of ICP27 and ORF57 involved in binding by REF have been mapped as residues 104–112 and 103–120, respectively. We have identified the pattern of residues critical for REF/Aly recognition, common to both ICP27 and ORF57. The importance of the key amino acid residues within these binding sites was confirmed by site-directed mutagenesis. The functional significance of the ORF57-REF/Aly interaction was also probed using an ex vivo cytoplasmic viral mRNA accumulation assay and this revealed that mutants that reduce the protein-protein interaction dramatically decrease the ability of ORF57 to mediate the nuclear export of intronless viral mRNA. Together these data precisely map amino acid residues responsible for the direct interactions between viral adaptors and cellular REF/Aly and provide the first molecular details of how herpes viruses access the cellular mRNA export pathway.


Published in the journal: Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57. PLoS Pathog 7(1): e32767. doi:10.1371/journal.ppat.1001244
Category: Research Article
doi: https://doi.org/10.1371/journal.ppat.1001244

Summary

The herpesvirus proteins HSV-1 ICP27 and HVS ORF57 promote viral mRNA export by utilizing the cellular mRNA export machinery. This function is triggered by binding to proteins of the transcription-export (TREX) complex, in particular to REF/Aly which directs viral mRNA to the TAP/NFX1 pathway and, subsequently, to the nuclear pore for export to the cytoplasm. Here we have determined the structure of the REF-ICP27 interaction interface at atomic-resolution and provided a detailed comparison of the binding interfaces between ICP27, ORF57 and REF using solution-state NMR. Despite the absence of any obvious sequence similarity, both viral proteins bind on the same site of the folded RRM domain of REF, via short but specific recognition sites. The regions of ICP27 and ORF57 involved in binding by REF have been mapped as residues 104–112 and 103–120, respectively. We have identified the pattern of residues critical for REF/Aly recognition, common to both ICP27 and ORF57. The importance of the key amino acid residues within these binding sites was confirmed by site-directed mutagenesis. The functional significance of the ORF57-REF/Aly interaction was also probed using an ex vivo cytoplasmic viral mRNA accumulation assay and this revealed that mutants that reduce the protein-protein interaction dramatically decrease the ability of ORF57 to mediate the nuclear export of intronless viral mRNA. Together these data precisely map amino acid residues responsible for the direct interactions between viral adaptors and cellular REF/Aly and provide the first molecular details of how herpes viruses access the cellular mRNA export pathway.

Introduction

All herpesviruses replicate in the host cell nucleus and therefore utilise the host cell's protein transcription and translation apparatus, while at the same time suppressing the correspondent cellular processes [1][5]. Crucially, non-spliced viral mRNA is directed into the cellular mRNA export machinery, thus bypassing the stringent cellular controls which normally ensure that only fully processed mRNA is exported from the nucleus to the cytoplasm. In an uninfected cell, the process of mRNA export is closely connected with mRNA processing and splicing, which in turn are coupled with transcription. Cellular mRNA export involves the assembly of a multi-protein transcription and export (TREX) complex containing the RNA export factor REF/Aly; this signals that processing is complete and the cellular mRNA is ready to be exported via a TAP/NXF1-mediated interaction with the nuclear pore [6][8]. TAP forms a heterodimer with p15 and binds nucleoporins via central and C-terminal UBA-like domains [9]. REF/Aly provides a crucial link between mRNA and TAP: the binding of mRNA and TAP to REF are mutually-exclusive. TAP binding to REF-mRNA complex triggers transfer of RNA from REF to TAP. While REF is bound, it switches TAP into a high-affinity binding mode for RNA [10]. Once the ribonucleoprotein complex reaches the nuclear pore, REF dissociates and the mRNA is transported to the cytoplasm [11]. It is also possible that other cellular mRNA export factors may fulfil a role similar to that of REF/Aly [12][14]. Unlike cellular mRNA, the herpesvirus mRNA is often unspliced, therefore it cannot acquire export marker proteins using the normal pathway, via coupled transcription, splicing and export. To facilitate the efficient export of intronless viral mRNA all herpesviridiae produce a multi-functional adaptor protein [15] that shuttles between the nucleus and cytoplasm [3], [4], [16], and bridges between the viral mRNA and components of the TREX complex such as REF/Aly, thus marking viral mRNAs for export via TAP/NFX1 [17], [18]. In Herpes Simplex Virus type I (HSV-1) the infected cell protein 27 (ICP27) acts as the adaptor [3], [19]. In Herpesvirus Saimiri (HVS), the prototype γ-2 herpesvirus with close similarity to human Kaposi's Sarcoma-associated herpesvirus (KSHV), this role is carried out by the ORF57 protein [4], [20][22].

The regions of ICP27 and ORF57 involved in REF binding have been studied by analysing the effects of polypeptide truncations. For ICP27 it has been inferred as amino acids (aa) 104–138 [19], [23]. Recent in vivo studies suggested that the RGG box aa 138–152, which is involved in viral mRNA binding [2], [24][26], is also involved in REF/Aly interactions [27]. However, earlier in vitro data indicate that the RGG region does not bind REF directly [19]. In ORF57 the interactions with REF and with viral mRNA were localised within aa 8–120 [22], [28], [29]. Thus the identified regions of ICP27 and ORF57 apparently perform a similar function (REF/Aly and viral mRNA binding), however these regions lack any obvious sequence similarity which would highlight a common REF-binding motif. Moreover, it was not known whether ICP27 and ORF57 bind REF in a similar way. A number of previous studies used deletion mutants of REF to locate viral binding sites [22], [30], [31], however in the absence of structural information at that time, these mutations inadvertently perturbed the spatial structure of REF. The solution structures of murine Aly containing only the folded RRM domain [32] and the functional fragment of REF2-I which contained both the RRM and N-terminal domains (residues 1–155) have since been determined and characterised [33]. The REF2-I RRM domain at the surface-exposed area of α-helices 1 and 2 contains overlapping secondary binding sites for TAP and UAP56/DDX39; in the free form this binding site is shielded by loose binding of the N-terminal helix [33]. Additionally, this RRM has a non-canonical secondary RNA-binding site comprised of the loop regions [33]. The site of viral adaptor binding however remained unknown, making it difficult to understand how the assembly of the viral mRNA-protein complex is achieved.

Here we apply NMR spectroscopy to explore the binding of ICP27 and ORF57 with REF at a residue-level resolution and report a side-by-side comparison of the essential peptide fragments of ORF57 and ICP27 required for binding with REF. We demonstrate that the REF recognition site of ICP27 is very short but highly specific. The atomic resolution structure of REF RRM domain bound with the fragment of the viral protein adaptor is presented. The respective REF-binding site on ORF57 is longer and includes several weaker points of contact. The two viral proteins however bind at the same site on the REF RRM domain, which overlaps with the secondary TAP-binding site. The identified key residues of ORF57 for its interaction with REF are confirmed by side-directed mutagenesis and ex vivo studies.

Results

Initial identification of binding domains

To confirm the position of REF-binding domains within ICP27 and ORF57 and to minimise the size of constructs for more detailed NMR mapping, a series of fragments derived from HSV-1 ICP27 were screened for binding to GST-REF2-I using pull down assays (Fig. 1). The binding of ICP27 aa 1–138 (ICP271–138) was essentially the same as that of the full-length protein, whereas ICP271–103 and ICP27139–512 showed no binding (Fig. 1B), confirming aa 104–138 contain the REF interaction site in vitro, in agreement with previous studies [19], [23]. The GST fusion of ICP27103–138 was found to interact similarly with full-length REF1–218, REF1–155 and REF54–155 under the given conditions, but only weakly with REF1–70. These data indicate aa 104–138 of ICP27 are necessary and sufficient for interaction with REF (Fig. 1C). The REF-binding fragment of ORF57 aa 8–120 identified previously [22] similarly interacted with the same fragments of REF (Fig. 1D). Unlike an earlier study [22], the REF54–155 construct used here does not perturb the structure of the RRM domain [33]. These experiments showed that the main binding sites for both viral proteins are located within the REF54–155 construct.

Identification of regions for the interactions between REF and ICP27/ORF57.
Fig. 1. Identification of regions for the interactions between REF and ICP27/ORF57.
(A) Subdivision of ICP27, ORF57 and REF2-I in fragments. (B) GST-REF pulls down ICP271–138 (NtS) as efficiently as full-length ICP27, but not ICP271–103 (Nt) and ICP27139–512 (Ct), indicating that residues 104–138 of ICP27 are involved in REF binding. (C) Fusion of the ICP27103–138 peptide to GST allows a specific pull down of REF, REF1–155 (NM) and REF54–155 (Δ53) and a very weak pull down of the REF1–70 (N); no interaction is detected with the control Ras protein. (D) Same pull down assay as panel C but using GST fusion of ORF57 aa 8–120 in place of ICP27.

To compare the mode of interaction of ICP27 and ORF57 with REF in more detail, NMR chemical shift mapping of backbone amides of 15N-labelled REF1–155 and REF54–155 was carried out (see Supporting Information available online, Fig. S1), by adding unlabelled ICP271–138 or ORF578–120 (Fig. S2) and monitoring signal shifts in 1H-15N correlation spectra. The sequence-specific signal assignment of REF in free form [34] was used to identify amino acids affected by binding. This indicated that while there may be a weak transient interaction with the N-terminal region of REF, especially for ORF57, the main interaction site is located in the REF RRM domain. The similar pattern of residues showing changes in chemical shifts induced by ICP27 and ORF57 indicated that both viral proteins bind REF on the same site (Supporting Information, Fig. S1E). No significant changes were observed in the 1H-15N correlation spectra of 15N-labelled REF156–218 upon addition of ICP271–138 or ORF578–120, confirming that the REF C-terminal domain is not involved in binding with these viral protein constructs (Fig. S1F). These initial studies thus confirm ICP27 and ORF57 bind REF in a similar manner.

Detailed mapping of binding sites

To identify which amino acids of ICP27103–138 and ORF578–120 bind REF54–155, the sequence-specific backbone assignment of free and bound forms of all these constructs was completed. Titrations were performed using additions of non-labelled polypeptides to 15N-labelled constructs, while monitoring spectral changes in 1H-15N correlation spectra. ICP27103–138/ORF578–120 was added to REF54–155, and vice versa. This enabled the mapping of interaction sites on all proteins at a residue-level resolution. The values of heteronuclear 15N{1H} NOE were also measured to identify the parts of polypeptide chains with altered mobility due to binding (data overview on Fig. 2, with the more detailed data included in the Supporting Information). Titration of REF with ICP27103–138 confirmed that this short peptide interacted with REF in the same manner as the longer ICP271–138 construct, and also in a similar manner as ORF578–120. The viral protein binding site on REF RRM was mapped to α-helices 1 and 2 plus the adjacent loop regions (Fig. 2A and Fig. S3). The converse titration showed that only a short section of ICP27103–138, namely aa 104–112, displayed chemical shift changes and decreased mobility, whereas the rest of the peptide remained flexible in complex (Fig. 2C and Fig. S4). Similarly, addition of REF54–155 caused significant changes in signal positions and signal broadening, along with decreased mobility, primarily within a short section of residues 103–120 of ORF578–120 (Fig. 2B and Fig. S5), the rest of the peptide was only weakly affected by binding. The REF-binding site of ORF57 thus appears to be significantly longer than that of ICP27, however both viral peptides bind to the same site on REF (Figs. 3A,B). Unlike the ICP27103–138 -REF54–155 complex, in the ORF578–120 - REF54–155 complex a number of signals are broadened beyond detection, indicating that the latter complex is in the intermediate chemical exchange regime and hence is not suitable for atomic-resolution structural studies.

Identifying interaction sites within REF, ORF57 and ICP27 constructs via different NMR parameters.
Fig. 2. Identifying interaction sites within REF, ORF57 and ICP27 constructs via different NMR parameters.
The detailed data is presented in SI. Purple solid and broken bars mark residues with large and moderate reduction of mobility upon binding, respectively, as evidenced by the increase in 15N{1H} NOE. Orange stars mark residues with amide signals broadened beyond detection in the complex. Large and moderate chemical shift changes (δCS) of amide signals for each ligand as labelled are shown as solid and broken bars, respectively. Red bars mark residues forming direct inter-molecular NOE contacts in REF-ICP27 complex. (A) Changes in REF54–155 induced by addition of ICP27103–138 and ORF578–120. Position of secondary structure elements of REF (β-sheets, α-helices and loops) is shown in relation to its sequence. ICP27 and ORF57 bind on the same site on the REF RRM domain, with α-helices 1 and 2 and adjacent loop regions. (B) Changes in ORF578–120 upon addition of REF54–155. The main REF-interaction site is mapped to residues 103–120. (C) Changes in ICP27103–138 induced by addition of REF54–155. The REF-binding site is mapped to residues 104-112.

Mapping of the ICP27- and ORF57-induced signal shifts onto the structure of REF RRM domain.
Fig. 3. Mapping of the ICP27- and ORF57-induced signal shifts onto the structure of REF RRM domain.
Backbone amide weighted chemical shift changes (δCS) are emphasized by colour. δCS >0.3 are red, 0.15–0.299 orange, 0.05–0.149 yellow, prolines are blue and regions unaffected are green. (A) Regions affected by addition of ICP27103–138. True ICP27 chain position determined based on NOE data is also shown for reference, with corresponded chemical shift changes due to REF binding similarly mapped. (B) Similar regions are affected by ORF578–120 and (C) synthetic peptide ICP27103–103. (D) Synthetic mutant peptide ICP27103–103 W105A binding affects the same RRM regions, however the value of shifts at the top of α-helix 1 is reduced, highlighting the binding site for W105 (encircled on panel C). The position and numbering of N- and C- terminal residues are indicated.

Structure of the REF-ICP27 complex

To obtain a detailed view of the ICP27103–138 interaction with REF54–155 we determined the atomic-resolution structure of this complex (Fig. 4A–G). The structure of the complex is well-defined owing to a large number of intra- and inter-molecular NOEs observed and assigned (Table 1; also Fig. S6). In agreement with the chemical shift mapping data, the viral peptide binds as a linear chain along the cleft formed by two α-helices on the surface of the RRM domain, which largely preserves its structure. However in the bound state the α-helix 1 of REF is shifted by approximately 3 Å (Fig. 4C). This shift causes some rearrangements within the looped regions, especially aa 136–146. These changes are accompanied by a noticeable decrease in mobility within the residues 86–90, 93–99, 132 and 137–145 as evidenced by 15N{1H} NOE data (Fig 2A). The shift in α-helix 1 exposes the sidechain of F98 for interaction with W105 of the peptide, and brings closer the sidechains of a hydrophobic patch composed of L94, Y135, V138, L140 and M145 (Fig. 4D, E). The upper edge of α-helix 1 contains a negatively charged patch of D90, E93 and E97 (Fig. 4F). The extended region aa 104–109 of ICP27 sitting along the REF cleft is followed by a loose bend at 109–112 which makes additional contacts with REF and points the remainder of the peptide chain away from the site. The region aa 114–138 remains flexible and hence does not participate in binding, in agreement with a lack of intermolecular NOEs and absence of signal shifts. From a structural perspective, three ICP27 residues appear most important for the interaction, forming a recognition triad (Fig. 4E). The sidechain of W105 makes hydrophobic contacts with F98 of REF and also with the aliphatic part of the R107 sidechain of ICP27. R107 forms salt bridges with the acidic residues of REF α-helix 1 (E93 and/or E97, the latter is the most likely binding partner in light of the decrease in mobility observed). L108 fits into a hydrophobic pocket composed of REF residues V86, L94, Y135, V138, L140, M145. Additionally V104 may also play a role in stabilising the complex, by forming hydrophobic contacts with the aliphatic parts of K130 and K133 of REF. The sidechain of S106 is solvent exposed and does not appear to directly bind REF. The ICP27 binding site shows a surprising degree of complementarity to REF, with a very short sequence used for highly specific recognition.

Structure of the REF - ICP27 complex.
Fig. 4. Structure of the REF - ICP27 complex.
(A) Ribbon representation showing ICP27 coloured blue, and REF RRM coloured green, red and yellow for looped, α-helical and β-sheet regions, respectively. Positions of N- and C-termini of polypeptide chains are labelled. (B) Overlay of 20 lowest energy structures with backbone shown in the same orientation, and rotated 180°. The best-fit superposition is made using heavy backbone atoms of structurally defined regions aa 74–152 of REF and 102–112 of ICP27. Colour-coding is the same as on panel A. (C) Overlay of the RRM domains of free REF2-I (red, PDB code 2F3J), free murine Aly (purple, PDB code 1NO8) and ICP27-bound REF2-I determined here (green), demonstrating the shift in α-helix 1 position. (D) Representation of REF – ICP27 complex in the same orientation with partially transparent surface. (E) Schematic of the REF and ICP27 binding site. ICP27 residues are coloured blue and REF in black; the hydrophobic and electrostatic interactions are indicated by dashes coloured green and red, respectively. (F) Electrostatic surface with negative and positive charge coloured red and blue, respectively. Bottom row shows for comparison the known structures of RRM domains (green) with bound peptide ligands (orange). (G) REF54–155 in complex with ICP27103–138, determined here. (H) UHM domain of human SPF45 in complex with SF3B155-ULM5 (PDB code 2peh, [44]). (I) U2AF35 in complex with U2AF65 (PDB code 1jmt, [42]).

Tab. 1. NMR calculation statistics for an ensemble of the 20 lowest energy structures of REF<sup>54–155</sup> bound to ICP27<sup>103–138</sup>.
NMR calculation statistics for an ensemble of the 20 lowest energy structures of REF&lt;sup&gt;54–155&lt;/sup&gt; bound to ICP27&lt;sup&gt;103–138&lt;/sup&gt;.

Molecular modelling of human Aly-ICP27 complex

Herpes simplex virus (HSV) causes common infections in humans that occur on the mouth and lips, including cold sores and fever blisters. Although murine REF2-I protein employed in this study is commonly used as a model to study mRNA export, potentially there may be differences in the way ICP27 recognises its native partner Aly, the human orthologue of murine REF. Here we explored this issue in detail. A sequence alignment of murine REF2-I, murine Aly (mAly) and human Aly (hsAly) (Fig. 5A) show very high level of homology within the RRM domains. Specifically there are seven amino acid substitutions between murine REF2-I and human Aly (Fig. 5A). However, only one of these substitutions lies within a binding site (Fig. 5B and C), namely V138 (which is a phenylalanine in human Aly). This conservative substitution is positioned on the periphery of the hydrophobic pocket that contacts L108 of ICP27. Molecular modelling of the structure of human Aly bound to ICP27 was performed to see how significantly the binding interface with ICP27 is affected by the differences in sequence (Fig. 5D and E). The modelling results show that the increase in hydrophobic sidechain volume of the V138F mutation could be readily accommodated by the movement of the sidechain of M145 (Fig. 5E). All other amino acid substitutions were positioned away from the binding interface. Comparison of modelled hsAly-ICP27 and experimental murine REF-ICP27 solution structure showed a heavy atom backbone RMSD of only 0.04 Å, with the architecture of ICP27 binding site maintained in both homologues. Therefore we conclude that ICP27 can bind to human Aly in the same manner as to murine REF2-I.

Modelling of complex between human Aly and HSV-ICP27.
Fig. 5. Modelling of complex between human Aly and HSV-ICP27.
(A) Sequence alignment of RRM domains of murine REF2-I (mREF2-I: CAB76384) used in this study, murine Aly (mALY: AAC53117) and human Aly (hALY: AAD09608). Position of secondary structure elements (β-sheets, α-helices and loops) is shown in relation to the REF2-I. Resides in mALY and mREF2-I that differ from hALY are highlighted in light red. (B) A cartoon of the structure of REF-ICP27 with the position of the 7 amino acid differences indicated using red space-fill spheres, this orientation shown is the same as used in Fig. 3, whereas in (C) an alternative orientation is used for clarity. Only one amino acid difference is part of the ICP27 interaction site, namely Val138 of mREF2-I, which is a Phe in hALY. (D) A model of hsALY (blue) overlaid with the experimental structure of mREF2-I (green), with ICP27 also shown (orange). Residues that differ between the RRM domains of mREF2-I and hsALY are red sticks in the mREF2-I form and cyan sticks in hsALY. The sidechain of M145 altered in the modelling procedure is indicated by sticks. Also the ICP27 sidechain of L108 is indicated (orange) which is positioned within the hydrophobic pocket of REF. (E) Detailed view of the sidechains of Met 145, and Val138 and Phe138 in the modelled hsALY (blue) and experimental REF2-I (green) structures, plus L108 of ICP27 is shown (orange).

Exploring the specificity of REF binding site of viral protein adaptors using synthetic peptides

The characteristic triad, Trp followed shortly by Arg and then by a hydrophobic residue, is also found in the REF-binding region of ORF57, and bears distant similarity to the sequences of some other viral protein adaptors (Table 2 and Fig. S2). To probe the specificity of recognition, 12 synthetic peptides were tested for binding with REF54–155 (see Table 2). The first set of peptides was derived from ICP27 and included WT ICP27103–112 and its three single point mutants W105A, R107A and L108A, plus a shorter WT ICP27103–110 peptide with two arginines removed. The second set was derived from HVS ORF57 and included WT HVS-ORF57103–120, and its W108A, R111A and V112A mutants, along with a shortened peptide WT HVS-ORF57105–115 designed to probe the minimal binding region of ORF57 for REF. Additionally, two peptides were chosen from other viral proteins with apparent sequence similarity but containing some variations in the triad residues, to test how well these can bind REF in our experiments. We used sequences from Varicella-zoster virus (VZV) ORF4108–119 (Tyr instead of Trp) and Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57100–110 (Tyr instead of Trp, and Lys instead of Arg). No prior data was available whether this VZV-ORF4 fragment binds to the RRM domain of REF. For KSHV-ORF57 a different region was previously implicated in binding with REF [35], [36], therefore the peptide KSHV-ORF57100–110 was not expected to interact and was used here as a negative control.

Tab. 2. Dissociation constants for the interaction of REF54–155 with viral protein fragments.
Dissociation constants for the interaction of REF<sup>54–155</sup> with viral protein fragments.
Sequences of synthetic and recombinant polypeptides tested here are shown aligned according to position of triad residues (highlighted bold). Continuation of amino acid sequence in the construct is indicated as ellipsis. Mutated residues are underlined.

A separate titration of each peptide was carried out under the same sample conditions. Increasing amounts of peptide were added to a 15N-REF54–155 sample, achieving binding saturation whenever possible. Throughout these titrations amide chemical shift changes were monitored to assess the relative binding affinity of these peptides for REF and simultaneously map their binding sites. Estimates of dissociation constants were obtained for each peptide, and for the longer viral adaptor fragments (Table 2). The WT ICP27 peptides 103–112 and 103–110 showed very similar binding characteristics to the longer aa103-138 construct, confirming that this peptide comprises the entire binding site (Fig. S3). The mutant peptide W105A showed reduced affinity to REF54–155 but still bound with similar chemical shift change pattern (Fig. 3D and Fig. S3). A reduction in affinity was more pronounced in the L108A mutant, with the R107A mutation virtually abolishing the binding. For the HVS peptides, ORF57103–120 bound with affinity comparable to ORF578–120, whereas affinity was decreased approximately two orders of magnitude in the shortened fragment WT-ORF57105–115. This agrees with the NMR mapping data that a longer sequence from ORF57 (residues 103–120) is involved in REF binding. The ORF57 mutant peptides R111A and V112A showed significantly reduced affinity for REF54–155, whereas the W108A mutant showed virtually no interaction. The VZV-ORF4108–119 peptide bound only weakly to REF54–155, whereas the KSHV-ORF57100–110 peptide used here as a negative control did not bind noticeably to REF (Table 2). These latter two viral adaptors lack the signature Trp residue, and additionally in KSHV-ORF57 the Arg within the triad is replaced by Lys. As a further control, we also checked if binding of the viral peptides is specific to the RRM domain of REF, or if it can occur with RRMs of other proteins as well. The proteins SF2 [37] and 9G8 [38] bind TAP/p15 and have roles in splicing and mRNA export somewhat similar to that of REF/Aly. They also contain an RRM domain and are therefore structurally homologous to REF/Aly. To test if the same ICP27 motif could interact with these RRM domains, we added a 5-fold excess of WT ICP27103–112 peptide to 15N-labelled SF2 [37] and 9G8 [38]. However no significant spectral changes and hence no binding was observed (Fig. S1). These experiments confirmed that the recognition of the REF RRM domain by viral adaptor proteins is highly specific, and the isolated peptides ICP27103–112, ICP27103–110 and ORF57103–120 are able to bind REF as efficiently as longer fragments of these viral proteins.

Probing the effect of phosphorylation of ICP27 using an S114E mutant

Recently it had been suggested that phosphorylation of S114 of ICP27 [39] may affect its interaction with REF. In the structure obtained here, this Ser is situated right on the edge of the binding interface. In order to probe the possible effect of its phosphorylation on the interaction with REF, the mutant ICP27103–138 S114E was produced to mimic the presence of the negative charge on the sidechain. Titration of 15N-labelled REF54–155 with unlabelled ICP27103–138 S114E revealed binding to the same site on REF (Fig. S3) and KD estimation showed that the affinity was only marginally different from the wild type ICP27103–138 construct (Table 2). To determine if the S114E mutation had any effect on the structure of the ICP27 construct used, we assigned and compared the fingerprint 1H-15N correlation spectra of 15N-labelled ICP27103–138 S114E mutant with that of the WT. The spectra overlaid well for all residues apart from residue 114 itself and its immediate sequential neighbours. According to 15N{1H} NOE measurement, both peptides were flexible in the free form, hence no structural changes were detected due to mutation. Titration with unlabeled REF54–155 indicated that the signals from the same region (aa 104–112) as WT ICP27 are most perturbed, with only a relatively small signal shift observed for E114 itself. These data suggest that there are no significant changes in direct binding of ICP27 to REF RRM in the mutant which mimics phosphorylation of S114.

Mutations of ORF57 and ICP27 residues identified by NMR affect binding of human Aly

To confirm the functional significance of critical residues within the REF binding site identified in ORF57 by chemical shift mapping experiments and analysis of synthetic peptide binding, a series of co-immunoprecipitation experiments were carried out using wild type and mutant forms of GFP-tagged full-length ORF57 and endogenous Aly in human cells (Fig. 6). Mutants were chosen that target the candidates for the recognition triad, as well as selected residues in the binding site and within the vicinity. All tested mutations within the proposed main binding site caused a significant decrease in ORF57-Aly affinity (namely, W108A, double R111A+V112A and R119A+R120A, and also triple W108A+R111A+V112A mutations, Fig. 6). The double mutants R79A+V80A and R94A+I95A situated outside the main binding site caused only a marginal if any decrease in Aly binding. These data corroborate the chemical shift mapping results and analysis of binding of synthetic peptide mutants, indicating that the main REF/Aly interaction site on ORF57 encompass aa 103–120, and confirms that triad residues W108, R111 and V112 of ORF57, in addition to R119 and R120, are important for the recognition of REF/Aly within the context of the functional full-length protein.

Functional importance of the identified REF binding site for the interaction of ORF57 with human Aly.
Fig. 6. Functional importance of the identified REF binding site for the interaction of ORF57 with human Aly.
(A) Co-immunoprecipitation assays were performed to compare the binding of wild type ORF57-GFP and ORF57 mutants. Site-directed mutations were made within the REF binding site of ORF57 and within its vicinity using Quik-Change II (Stratagene), according to the manufacturer's instructions. 293T cells were transfected with GFP-tagged ORF57 and mutant proteins and after 24 hours cell lysates were incubated with Protein A agarose and a polyclonal GFP-specific antibody and precipitated proteins were analysed by Western blotting with Aly-specific antibody and monoclonal antibodies to GFP as a loading control. (B) The relative binding affinities in repeated experiments were analysed quantitatively by densitometry. Point mutations affecting recognition triad residues 108, 111 and 112 and double arginines 119,120 all significantly reduce ORF57-Aly interaction. Mutations of similar type outside the interaction site have not affected the interaction between the two proteins.

Similar co-immunoprecipitation experiments were performed using wild type and mutant forms of full-length ICP27, specifically mutating W105A, R107A+L108A and W105A+R107A+L108A. Results demonstrate that all three mutants showed a significant reduction in Aly binding, again corroborating data obtained by chemical shift mapping and analysis of binding of synthetic peptide mutants (Fig. 7). The co-immunoprecipitation experiments for both ORF57 and ICP27 confirm that the REF-binding sites characterized here in detail using shorter polypeptide constructs are also functionally important for the interaction of these proteins with Aly/REF in their full-length native forms.

Functional importance of triad residues 105, 107 and 108 for the interaction of ICP27 with human Aly.
Fig. 7. Functional importance of triad residues 105, 107 and 108 for the interaction of ICP27 with human Aly.
(A) Co-immunoprecipitation assays were performed on 293T cells transfected with GFP-tagged ICP27 and its mutants. Mutations probed triad residues identified in the experimental structure formed between short protein fragments of ICP27 and REF. Cell lysates were incubated with Protein A agarose and a polyclonal GFP-specific antibody and precipitated proteins were analyzed by Western blotting with Aly-specific antibody and a monoclonal antibody specific to GFP as a loading control. (B) The relative binding affinities from 3 independent experiments were analysed quantitatively by densitometry. Point mutations of the REF recognition triad residues all significantly decrease binding between full-length ICP27 and endogenous Aly, confirming the functional significance of these residues identified by NMR.

Mutations of HVS ORF57 residues important for REF/Aly binding affect ORF57-mediated cytoplasmic accumulation of mRNA

The functional importance of ORF57 residues within REF-binding site were also measured via an ex vivo assay for cytoplasmic accumulation of an HVS ORF47 reporter mRNA (Fig. 8), using wild type and mutant ORF57 proteins, as previously described [36], [40]. As such, the cytoplasmic accumulation detected in this assay reflects the ability of ORF57 to form an export competent ribonucleoprotein particle. Human 293T cells were transfected with pORF47 (a plasmid expressing the late intronless ORF47 mRNA) in the presence of wild type or mutant ORF57 proteins. After 24 hours RNA was extracted from cytoplasmic fractions and levels assessed by qRT-PCR. The mutation of residues directly implicated in the REF/Aly interaction, namely W108A, R111A+V112A and R119A+R120A, and also W108A+R111A+V112A, all substantially reduced the efficiency of the mRNA cytoplasmic accumulation. In addition, mutations of residues outside the primary REF-binding site were tested. Mutation R94A+I95A also similarly reduced cytoplasmic accumulation, whereas R79A+V80A caused only a marginal decrease. R94 is situated just outside the main REF-binding site and is part of the nuclear localization signal, and the observed effect can be possibly explained by its involvement in the interaction with viral mRNA and/or perturbed nuclear localization. The small effect of R79 substitution may be due to possible changes in mRNA binding. The results of these ex vivo experiments confirm the functional importance of individual residues identified by NMR for specific binding in the context of native Aly and full-length ORF57. Moreover, the results suggest that these individual residues critical for the HVS ORF57 – REF/Aly interaction are also required to enable efficient cytoplasmic accumulation of viral mRNA in our assay. This confirms the functional significance of ORF57 – REF/Aly interaction for ORF57-mediated nuclear export of viral intronless transcripts, leading to recruitment of other hTREX proteins [40] and TAP.

Perturbing the ORF57-REF/Aly interaction with point mutations decreases cytoplasmic accumulation of viral mRNA.
Fig. 8. Perturbing the ORF57-REF/Aly interaction with point mutations decreases cytoplasmic accumulation of viral mRNA.
293T cells were transfected with the pORF47 reporter mRNA construct and the vectors containing wild type ORF57 or its mutants, and RNA was isolated from cytoplasmic fractions. The mutations probed residues from REF binding site, as well as from its vicinity. qRT-PCR was performed and data for mRNA reporter plus ORF57-transfected cells normalised against cells transfected with reporter in the presence of GFP. A ΔΔcT method was applied to determine the relative levels of reporter mRNA between samples. Point mutations of ORF57 residues implicated in direct interaction with REF, and reducing its binding, all caused significant decrease in cytoplasmic accumulation of viral mRNA.

Discussion

The use of NMR with short optimised constructs of REF, HSV-1 ICP27 and HVS ORF57 has allowed the precise determination of the residues important for the recognition of viral proteins by the cellular mRNA export factor REF. Despite the lack of obvious sequence similarity, both viral proteins bind on the same main site, along the cleft formed by the two α-helices in the RRM domain of REF. Our data shows that for ICP27 a short but highly specific amino acid sequence 103–110 is required and sufficient for REF-binding (with residues 105, 107 and 108 being critical). This region is immediately followed by a nuclear localization sequence (NLS) aa110-137 [41], without a significant overlap between the two. Within the ORF57 protein, the REF-interaction sequence is significantly longer and includes aa 103–120. The REF interaction sites of both ICP27 and ORF57 proteins contain a recognisable triad pattern, a Trp shortly followed by an Arg-Leu/Val pair, which proved to be essential for REF binding. Mutation of these critical triad residues both in ICP27 and in ORF57 significantly reduced binding with REF. The insertion of an additional residue within this triad (as in the case of ORF57) distorts the complementarity of the binding interface and likely necessitates the presence of additional compensating contacts (i.e., via R119 and R120) and hence a longer recognition site. This is supported by chemical shift mapping, effect of peptide truncation on Kd and a change in REF binding for the R119,120A double mutant. The critical REF recognition residues were first identified and characterized in detail using relatively short protein constructs, confirming high specificity of detected interactions. It was however important to show that these REF recognition sites also work in the full-length native proteins. Here we demonstrate that the mutations of residues from recognition triads significantly reduced binding between full-length viral ICP27 and ORF57 and human Aly in co-immunoprecipitation assays, confirming functional significance of detected binding sites for proteins in their native form in nearly physiological conditions, both for ORF57 and ICP27.

The REF recognition site on ICP27 involves residues 103 to 110 (possibly extended to 112) and in our experiments it is entirely sufficient for highly specific binding with REF in vitro. Based on the interpretation of in vivo experiments, recently it had been suggested that phosphorylation of S114 [39] or modifications within the RNA-binding RGG motif aa 138–152 [27] affect the ICP27 interaction with REF. In the structure presented here, S114 is positioned very close to the binding site, but not immobilised upon binding. In principle, one can envisage that phosphorylation of this residue can make an additional favourable Coulombic contact with K133 and/or K136 of REF, immobilizing phosphoserine and strengthening the complex further. We have checked this hypothesis here by using a S114E mutant as a phosphoserine mimic. Both WT-ICP27103–138 and ICP27103–138S114E interact with REF with similar KD, the mutant interacts only marginally stronger (Table 2). This insignificant change in affinity observed in our experiments and the position at the periphery of the REF-binding motif suggests that it is unlikely that modifications of S114 can provide a direct stringent control of the REF-ICP27 interaction. This agrees with the observation that the S114A mutant still co-immunoprecipitates with REF/Aly [39]. Similarly, the RNA-binding RGG motif (aa 138–152) is positioned sequentially away from the specific REF-binding motif. Modifications in this RGG motif are unlikely to have a direct effect on ICP27-REF binding. The effects of ICP27 modifications outside the main 103–110 site on REF binding recently observed in vivo [27], [39] can be alternatively explained by trapping the mutated ICP27 in complexes upstream of the pathway, as suggested by [39]. Additionally, modifications within the RGG region of ICP27 may affect binding with RNA; this could indirectly affect ICP27-REF affinity if the RNA bridges the two proteins. Further experiments, which take into account cellular availability of modified ICP27 for interaction with REF/Aly and the bridging role of mRNA, are needed to reconcile the in vivo and in vitro data.

Here we have presented the first atomic-resolution structure of the complex between the fragment of archetype viral signature protein, ICP27, and the cellular export factor REF2-I. The ICP27 peptide binds on the α-helical side of the RRM domain, along the crevice between α-helices. The position of this peptide is defined by the presence of multiple unambiguous NOE contacts, which in particular pinpoint the position of the W105 and L108 of ICP27 (Fig. S6D and Fig. S7) and therefore align the peptide along the crevice. The corresponding 3D structure of human Aly bound to ICP27 is currently unknown, but within the ICP27 binding site the two proteins differ by just one amino acid residue in position 138, with Phe for Aly and Val for REF2-I. This site is situated on the periphery of the hydrophobic patch which interacts with L108 of ICP27 (Fig. 5). Comparative modelling of human Aly suggested the mutation could be easily accommodated without disrupting the interaction, therefore the complex between human Aly and ICP27 is likely to be structurally very similar to the one between REF2-I and ICP27 determined here. This modelling provides a molecular level insight into how the ICP27 protein from Herpes Simplex virus may interact with human Aly, to facilitate the nuclear export of herpesviral intronless mRNA, an essential prerequisite for virus replication.

The previous examples of peptides bound on the α-helical side of RRM-type domains differ from the structure described here. The U2AF homology motifs (UHM) have been shown to recognize a Trp residue which is preceded by a stretch of basic residues [42][44]. In the UHM-type of recognition, the signature Trp sidechain of the peptide is inserted into the hydrophobic pocket formed mainly by the looped regions, with the bound peptide running almost perpendicular to the crevice between the two α-helices (Fig. 4H,I). The characteristic Arg-X-Phe motif situated in the loop shortly after α-helix 2 is the defining signature of UHMs and is the key to Trp recognition [43]. The RRM of REF2-I clearly lacks this motif, and therefore does not belong to UHM class. Moreover, the similar hydrophobic pocket in the REF RRM is occupied by Leu108 of ICP27, and not by Trp (Fig. 4G–I). Interestingly, the presence of the Trp appears not to be as crucial as the other triad residues involved in ICP27 recognition, as its mutation reduces binding only one order of magnitude (Table 2). Residues more important for ICP27 binding are Arg107 and Leu108. Unlike in UHM recognition, in the REF - ICP27 complex the Trp makes contacts mainly with the top of α-helix 1, and middle part of α-helix 2. Both the abundant NOE contacts (Figs. S6 and S7) and relative perturbations caused by the W105A mutation (Fig. 3C,D), all consistently indicate that the mode of ICP27 binding with REF is different from peptide recognition by UHMs. Recently another apparently similar complex between PTB-RRM2 and Raver1 peptide has been described by NMR and modelling [45], where a crucial Leu-Leu pair of the LLGxxP motif is inserted in the binding pocket in the loops adjacent to α-helix 2. In this modelled complex the peptide also has a different orientation, compared with our structure based on direct NOE restraints, and interacting motifs have little similarity. Therefore, the structure presented here displays another, previously undescribed, mode of peptide-RRM recognition, adding to the previously recognized diversity of RRM-ligand interactions [46].

Previously, the position of viral mRNA binding sites on ORF57 has been loosely mapped to aa8-120 [22], [28], [29]. As the REF binding site aa103-120 is situated within the same fragment, it is not clear yet whether RNA and REF/Aly binding to ORF57 occurs concurrently or cooperatively. Our further studies are aimed at clarifying this. In the case of ICP27, the viral mRNA binding site is situated within the RGG region shortly following the REF-binding site. One can therefore anticipate that ICP27 brings and introduces the viral mRNA to REF/Aly, which can bind both ICP27 (via RRM domain) and viral mRNA (via N- and C-termini) simultaneously, ensuring a multi-contact interaction interface.

Here we demonstrated that point mutants in positions 108, 111, 112, 119 and 120 that reduce the ORF57 - REF/Aly interaction also dramatically decrease the ability of ORF57 to promote the nuclear export of intronless viral mRNA. Therefore these residues are functionally important for mRNA export, likely by directly mediating recruitment of REF/Aly. The ability of ORF57 and homologues to interact with export adapter proteins, such as REF/Aly, and possibly functional homologues such as UIF [13], is therefore likely to be essential for the formation of an export competent ribonucleoprotein particle. This in turn is essential for efficient viral mRNA nuclear export and subsequent virus replication, as we have previously demonstrated that recruitment of the complete hTREX complex to viral intronless mRNAs is essential for both HVS and KSHV lytic virus replication [29], [35]. Similarly, mutations of ICP27 residues in positions 105, 107 and 108 have also been shown here to decrease the interaction between full-length ICP27 and human Aly. Further experiments are needed to confirm the effect of mutations of recognition triad residues on the viral mRNA export mediated by ICP27. The functional role of the REF/Aly binding regions in ICP27 export to the cytoplasm have been studied previously by deletion of polypeptide fragments. Specifically, ICP27 deletions 64–108 (d2-3) and 109–138 (d3-4) were used and interpreted as mutants perturbing interaction with REF/Aly [17]. The current work suggests that in fact only the first of these two deletions affected the REF/Aly recognition triad. In the second d3-4 construct the main REF/Aly binding site was completely preserved, while the NLS was perturbed. This may explain why the d3-4 mutant maintained efficient export of ICP27 to the cytoplasm [17] – the interaction of this construct with REF/Aly was in reality possible. Moreover, the deletion constructs said to be lacking the REF/Aly binding site and used to demonstrate the absence of REF/Aly bridging between ICP27 and TAP/NXF1 [17], in fact, inadvertently preserved this site. In view of the detailed data presented here on the exact point mutations (residues 105, 107 and 108) which will perturb interactions with REF/Aly without affecting the NLS aa110-137, further functional studies may be warranted to reconsider the suggested diminished roles of the ICP27 - REF/Aly interaction in cytoplasmic export of ICP27, and of REF/Aly in mediating interactions with TAP/NXF1 [17]. Such studies however should consider the possibility that other adaptor proteins [12][14] may substitute the function of REF/Aly in vivo once the ICP27 – REF/Aly interaction is blocked, complicating the analysis. Additional experiments are also required to assess and map interaction of ICP27 with functional homologues of REF/Aly such as the recently identified UIF protein [13], to explore the role of alternative pathways. Regardless of how essential the REF-viral protein interaction appears from siRNA evidence [14], the recruitment of the ubiquitously-present cellular export factor Aly/REF to viral ICP27/ORF57 can be envisaged as a highly useful pathway linkage, increasing an overall efficiency of viral mRNA export, due to the ability of this export factor to remodel TAP triggering high affinity RNA - TAP interactions [10]. The main interaction site for TAP on REF is an N-terminal arginine rich motif; however, the REF RRM also contributes to TAP interactions [10], [33] and this secondary site overlaps with the site recognised by ICP27 and ORF57. Therefore TAP recruitment is likely to lead to remodelling of the viral ribonucleoprotein complex. Although the partial displacement of the viral adaptor fragment from the surface of RRM of REF upon TAP binding may be possible, the complete displacement of the viral proteins from the ribonucleoprotein complex seems unlikely since a ternary complex of REF - TAP and ORF57/ICP27 assembles in vitro [19], [22]. Further studies are required to establish how the viral mRNA export complex is remodelled during export and which proteins contact the viral mRNA directly at each point in the export pathway.

Materials and Methods

Protein expression and purification

Constructs REF1–155, REF54–155, ICP271–138 and ORF578–120, expressed in pET24b (Novagen) vector, were produced as described previously [33], with additional purification on a Superdex 75 (GE Healthcare) column (GF buffer: 20 mM phosphate, 150 mM NaCl, 50 mM L-Arg/L-Glu/β-mercaptoethanol and 10 mM EDTA, pH 6.2). Proteins SF2 and 9G8 were purified as described previously [37], [38]. ICP27103–138 WT and S114E peptides were expressed as GST-fusions in a pGEX-6P-1 plasmid, and cleaved by PreScission protease on GSH resin according to standard protocol (GE Healthcare). Eluted peptide was supplemented with 5 mM DTT and protease inhibitor cocktail (Roche), and exchanged into GF buffer using an Amicon pressure cell with 1 k MWCO membrane via a series of dilutions/concentrations. A Sephacryl S-100 HR (GE Healthcare) gel filtration column was used to purify the peptide further. Peptide was >95% pure according to tricine-SDS-PAGE.

Pull down assays and co-immunoprecipitations

GST or GST protein fusions were first immobilised on 30 µl slurry glutathione-coated beads (GE Healthcare) before 8 µl 35S-radiolabelled proteins synthesised in rabbit reticulocytes (Promega) were added to the binding reactions in RB100 buffer (25 mM HEPES pH 7.5/100 mM KOAc/10 mM MgCl2/1 mM DTT/0.05% Triton X-100/10% glycerol) in presence of 10 µg/ml RNAse A. Washed and eluted protein complexes were resolved on 15% SDS-PAGE stained with Coomassie blue and analysed by PhosphoImage. To analyse the effect of HVS ORF57 and HSV-1 ICP27 point mutations on Aly/REF binding, co-immunoprecipitation were performed as previously described [47], [48]. Human 293T cells were transfected with wild type GFP-ORF57 or GFP-ICP27 and respective mutants, generated using the QuickChange II site-directed mutagenesis kit (Stratagene), using Lipofectamine 2000 (Invitrogen, Paisley, UK), as per the manufacturer's instructions. Briefly, after 24 hours, cell lysates were harvested, precleared with Protein A agarose for 1 hour at 4°C and then incubated with polyclonal GFP-specific antibody for 2 hours at 4°C. Protein A agarose was added to the cell lysates and incubated for a further 3 hours at 4°C. The agarose was washed 3 times to remove unbound protein. Western blot analysis was then performed using an Aly-specific antibody and GFP-specific monoclonal antibody as a loading control. Densitometry analysis was then performed on 3 independent experiments using the ImageJ software.

Cytoplasmic mRNA accumulation assay

293T cells were transfected with ORF57 or the respective mutants in the presence of the pORF47 reporter mRNA as previously described [49]. Cytoplasmic ORF47 mRNA levels were then assessed by qRT-PCR as previously described [36]. Briefly, after 24 hours, cells were lysed in 200 µl of PBS 1% Triton-X 100 (v/v) containing 40 U of RNAse Out (Invitrogen), and cytoplasmic fractions isolated using Trizol (Invitrogen) as previously described [36]. Total RNA (1 µg) from each fraction was reverse transcribed using Superscript II (Invitrogen) and 10 ng of cDNA used as template in SensiMixPlus SYBR qRT-PCR reactions (Quantace). qPCR was performed using the Rotor-Gene Q 5plex HRM Platform (Qiagen), with a standard 3-step melt program (95°C melt for 30 sec, 60°C annealing for 15 secs, 72°C extension for 20 secs). Following confirmation that qPCR efficiency was comparative between ORF47 and the reference mRNA (GAPDH), quantitative analysis was performed using ΔΔcT analysis as previously described [36].

NMR experiments

All experiments were carried out at 30°C on Bruker DRX600, DRX700 and Varian Inova 800 MHz spectrometers equipped with cryoprobes. The weighted chemical shift changes of amide signals δCS caused by complex formation were measured as , where ΔδH and ΔδN were changes in proton and nitrogen chemical shifts, respectively. Standard triple-resonance experiments were used to assign spectra of ICP27103–138, ORF578–120 and REF54–155 in their free and bound states. Additionally, carbon-detection experiments (CON, CaCO, CbCaCO, CbCaCO(N), CbCaNCO) were used as an aid to the ORF57 assignment. Spectra were processed using NMRpipe [50] and Topspin 2.1 (Bruker) and analysed using Sparky (University of California). Distance restraints obtained from 3D 15N- and 13C edited NOESY-HSQC experiments (τm 120 ms) and dihedral restraints from TALOS [51] were used in structure calculations by CYANA [52]. Additionally, intermolecular contacts were unambiguously identified using 13C edited, 12C-filtered NOESY-HSQC (τm 150 ms) spectra acquired on Varian Inova 800 MHz spectrometer. In this experiment only NOE crosspeaks between 1H-13C moieties of 13C,15N-labelled REF and 1H(12C) of unlabelled ICP27 peptide were observed [53], [54]. A final ensemble contained 20 structures with lowest target function values. Images were generated using Pymol (DeLano Scientific). For the REF - ICP27 complex, structure coordinates and experimental constraints have been deposited into the Protein Data Bank and chemical shifts in the BioMagResBank (access numbers 2kt5 and bmr16683 respectively). Other chemical shift assignments deposited in the BioMagResBank are: free ICP27103–138 bmr16696, free REF54–155 bmr16697, and ORF578–120 bmr16698 (both free and bound to REF54–155).

Unlabelled synthetic peptides were obtained from Peptide Protein Research Ltd (UK). The synthetic peptide sequences used were from Varicella-zoster virus ORF4108–119 (AAY57694), Kaposi's sarcoma-associated herpesvirus ORF57100–110 (YP_001129410), Herpesvirus Saimiri ORF57103–120 & 105–115 (CAC84353), Herpes simplex virus 1 ICP27103–110 & 103–112 (AAF43147). Dissociation constants (KD) were derived by monitoring chemical shift changes in 1H-15N correlation spectra of 100 µM 15N-REF54–155 as a function of increasing peptide concentrations, and fitting data to the standard equation [55]. For very weak binding peptides where chemical shift changes were too small to obtain a curve for fitting, a lower limit estimate of KD was obtained by comparisons of the magnitude of chemical shift change observed relative to those of stronger complexes.

Molecular modeling

Comparative modelling of the human Aly – ICP27 complex was performed using Swiss-PdbViewer [56] and the lowest energy conformer of the REF - ICP27 complex as a template. Mutations A75G, D107H, D119N, R125K, K136N, V138F and D146N (which reflect the differences between murine REF2-I and human Aly within the RRM domain) were introduced. All mutations except V138F involved solvent exposed sites and did not cause steric clashes. For the V138F substitution, a conformation was chosen that minimised the number of steric clashes while orientating the aromatic sidechain towards the hydrophobic core of REF. An energy minimization was conducted to remove the remaining steric clash with the ε-methyl of M145, resulting in a change in the M145 side chain rotamer, and virtually no movement of the backbone (heavy atom backbone RMSD of 0.04 Å).

Supporting Information

Attachment 1

Attachment 2

Attachment 3

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Attachment 7


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Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium

Článok vyšiel v časopise

PLOS Pathogens


2011 Číslo 1
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