#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#
preLekára.sk / Odborné časopisy / PLOS Genetics / 2013 - 2

Single Transmembrane Peptide DinQ Modulates Membrane-Dependent Activities

English version České info

The functions of several SOS regulated genes in Escherichia coli are still unknown, including dinQ. In this work we characterize dinQ and two small RNAs, agrA and agrB, with antisense complementarity to dinQ. Northern analysis revealed five dinQ transcripts, but only one transcript (+44) is actively translated. The +44 dinQ transcript translates into a toxic single transmembrane peptide localized in the inner membrane. AgrB regulates dinQ RNA by RNA interference to counteract DinQ toxicity. Thus the dinQ-agr locus shows the classical features of a type I TA system and has many similarities to the tisB-istR locus. DinQ overexpression depolarizes the cell membrane and decreases the intracellular ATP concentration, demonstrating that DinQ can modulate membrane-dependent processes. Augmented DinQ strongly inhibits marker transfer by Hfr conjugation, indicating a role in recombination. Furthermore, DinQ affects transformation of nucleoid morphology in response to UV damage. We hypothesize that DinQ is a transmembrane peptide that modulates membrane-dependent activities such as nucleoid compaction and recombination.

Vyšlo v časopise: Single Transmembrane Peptide DinQ Modulates Membrane-Dependent Activities. PLoS Genet 9(2): e32767. doi:10.1371/journal.pgen.1003260
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1003260

Souhrn

The functions of several SOS regulated genes in Escherichia coli are still unknown, including dinQ. In this work we characterize dinQ and two small RNAs, agrA and agrB, with antisense complementarity to dinQ. Northern analysis revealed five dinQ transcripts, but only one transcript (+44) is actively translated. The +44 dinQ transcript translates into a toxic single transmembrane peptide localized in the inner membrane. AgrB regulates dinQ RNA by RNA interference to counteract DinQ toxicity. Thus the dinQ-agr locus shows the classical features of a type I TA system and has many similarities to the tisB-istR locus. DinQ overexpression depolarizes the cell membrane and decreases the intracellular ATP concentration, demonstrating that DinQ can modulate membrane-dependent processes. Augmented DinQ strongly inhibits marker transfer by Hfr conjugation, indicating a role in recombination. Furthermore, DinQ affects transformation of nucleoid morphology in response to UV damage. We hypothesize that DinQ is a transmembrane peptide that modulates membrane-dependent activities such as nucleoid compaction and recombination.

Zdroje

1. Fernandez De HenestrosaAR, OgiT, AoyagiS, ChafinD, HayesJJ, OhmoriH, et al. (2000) Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol Microbiol 35 : 1560–1572.

2. CourcelleJ, KhodurskyA, PeterB, BrownPO, HanawaltPC (2001) Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158 : 41–64.

3. ZuberP (2001) A peptide profile of the Bacillus subtilis genome. Peptides 22 : 1555–1577 doi:DOI:10.1016/S0196-9781(01)00492-2.

4. IbrahimM, NicolasP, BessieresP, BolotinA, MonnetV, et al. (2007) A genome-wide survey of short coding sequences in streptococci. Microbiology 153 : 3631–3644.

5. AlixE, Blanc-PotardABa (2009) Hydrophobic peptides: novel regulators within bacterial membrane. Mol Microbiol 72 : 5–1110.1111/j.1365-2958.2009.06626.x.

6. HemmMR, PaulBJ, SchneiderTD, StorzG, RuddKE (2008) Small membrane proteins found by comparative genomics and ribosome binding site models. Mol Microbiol 70 : 1487–1501.

7. HemmMR, PaulBJ, Miranda-RiosJ, ZhangA, SoltanzadN, et al. (2010) Small Stress Response Proteins in Escherichia coli: Proteins Missed by Classical Proteomic Studies. J Bacteriol 192 : 46–58.

8. BishopRE, LeskiwBK, HodgesRS, KayCM, WeinerJH (1998) The entericidin locus of Escherichia coli and its implications for programmed bacterial cell death. J Mol Biol 280 : 583–596 doi:DOI: 10.1006/jmbi.1998.1894.

9. GasselM, MöllenkampT, PuppeW, AltendorfK (1999) The KdpF Subunit Is Part of the K+-translocating Kdp Complex of Escherichia coli and Is Responsible for Stabilization of the Complex in Vitro. J Biol Chem 274 : 37901–37907.

10. WongRS, McMurryLM, LevySB (2000) ‘Intergenic’ blr gene in Escherichia coli encodes a 41-residue membrane protein affecting intrinsic susceptibility to certain inhibitors of peptidoglycan synthesis. Mol Microbiol 37 : 364–370.

11. ColeC, BarberJD, BartonGJ (2008) The Jpred 3 secondary structure prediction server. Nucleic Acids Res 36: W197–W201.

12. Weel-SneveR, BjorasM, KristiansenKI (2008) Overexpression of the LexA-regulated tisAB RNA in E. coli inhibits SOS functions; implications for regulation of the SOS response. Nucleic Acids Res 36 : 6249–6259.

13. WickensHJ, PinneyRJ, MasonDJ, GantVA (2000) Flow Cytometric Investigation of Filamentation, Membrane Patency, and Membrane Potential in Escherichia coli following Ciprofloxacin Exposure. Antimicrob Agents Chemother 44 : 682–687.

14. OdsbuI, Morigen, SkarstadK (2009) A Reduction in Ribonucleotide Reductase Activity Slows Down the Chromosome Replication Fork but Does Not Change Its Localization. PLoS ONE 4: e7617 doi:10.1371/journal.pone.0007617.

15. VogelJ, ArgamanL, WagnerEG, AltuviaS (2004) The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide. Curr Biol 14 : 2271–2276.

16. DarfeuilleF, UnosonC, VogelJr, WagnerEG (2007) An Antisense RNA Inhibits Translation by Competing with Standby Ribosomes. Mol Cell 26 : 381–392 doi:10.1016/j.molcel.2007.04.003.

17. KawanoM, OshimaT, KasaiH, MoriH (2002) Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Mol Microbiol 45 : 333–349.

18. EguchiY, ItouJ, YamaneM, DemizuR, YamatoF, Okada, et al. (2007) B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli. PNAS 104 : 18712–18717.

19. AlixE, Blanc-PotardAB (2008) Peptide-assisted degradation of the Salmonella MgtC virulence factor. EMBO J 27 : 546–557.

20. UnosonC, WagnerEG (2008) A small SOS-induced toxin is targeted against the inner membrane in Escherichia coli. Mol Microbiol 70 : 258–270.

21. Levin-ZaidmanS, Frenkiel-KrispinD, ShimoniE, SabanayI, WolfSG, MinskyA (2000) Ordered intracellular RecA-DNA assemblies: A potential site of in vivo RecA-mediated activities. Proc Natl Acad Sci U S A 97 : 6791–6796.

22. ModellJW, HopkinsAC, LaubMT (2011) A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW. Genes & Development 25 : 1328–1343.

23. BarbeJ, VillaverdeA, CairoJ, GuerreroR (1986) ATP hydrolysis during SOS induction in Escherichia coli. J Bacteriol 167 : 1055–1057.

24. KamensekS, PodlesekZ, GillorO, Zgur-BertokD (2010) Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogeneous expression. BMC Microbiol 10 : 283 1471-2180-10-283 [pii];10.1186/1471-2180-10-283 [doi].

25. McCoolJD, LongE, PetrosinoJF, SandlerHA, RosenbergSM, et al. (2004) Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy. Mol Microbiol 53 : 1343–1357 10.1111/j.1365-2958.2004.04225.x [doi];MMI4225 [pii].

26. FriedmanN, VardiS, RonenM, AlonU, StavansJ (2005) Precise temporal modulation in the response of the SOS DNA repair network in individual bacteria. PLoS Biol 3: e238 doi:10.1371/journal.pbio.0030238.

27. YamaguchiY, ParkJH, InouyeM (2011) Toxin-antitoxin systems in bacteria and archaea. Annu Rev Genet 45 : 61–79 10.1146/annurev-genet-110410-132412 [doi].

28. DörrT, Vuli-çM, LewisK (2010) Ciprofloxacin Causes Persister Formation by Inducing the TisB toxin in Escherichia coli. PLoS Biol 8: e1000317 doi:10.1371/journal.pbio.1000317.

29. DewittSK, AdelbergEA (1962) The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics 47 : 577–585.

30. DatsenkoKA, WannerBL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97 : 6640–6645.

31. WilsonGG, YoungKY, EdlinGJ, KonigsbergW (1979) High-frequency generalised transduction by bacteriophage T4. Nature 280 : 80–82.

32. BrosiusJ (1984) Plasmid vectors for the selection of promoters. Gene 27 : 151–160 doi:DOI: 10.1016/0378-1119(84)90136-7.

33. SeebergE, StrikeP (1976) Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmolyzed cells of Escherichia coli. J Bacteriol 125 : 787–795.

34. ClarkDJ, MaaløeO (1967) DNA replication and the division cycle in Escherichia coli. J Mol Biol 23 : 99–112 doi:DOI: 10.1016/S0022-2836(67)80070-6.

Štítky
Genetika Reprodukčná medicína
Článek Complex Inheritance of Melanoma and Pigmentation of Coat and Skin in Grey Horses
Článek A Meta-Analysis of Thyroid-Related Traits Reveals Novel Loci and Gender-Specific Differences in the Regulation of Thyroid Function
Článek Genetic Landscape of Open Chromatin in Yeast
Článek Deleterious Alleles in the Human Genome Are on Average Younger Than Neutral Alleles of the Same Frequency
Článek Age-Dependent Transition from Cell-Level to Population-Level Control in Murine Intestinal Homeostasis Revealed by Coalescence Analysis
Článek Next-Generation Sequencing Identifies the Danforth's Short Tail Mouse Mutation as a Retrotransposon Insertion Affecting Expression
Článek ImmunoChip Study Implicates Antigen Presentation to T Cells in Narcolepsy
Článek Massive Mitochondrial Gene Transfer in a Parasitic Flowering Plant Clade
Článek Comment on “Genomic Hypomethylation in the Human Germline Associates with Selective Structural Mutability in the Human Genome”
Článek The Prefoldin Bud27 Mediates the Assembly of the Eukaryotic RNA Polymerases in an Rpb5-Dependent Manner
Článek Genetic Determinants of Trabecular and Cortical Volumetric Bone Mineral Densities and Bone Microstructure
Článek Encodes a Novel and -Genus-Specific Regulator of Photoperiodic Flowering in Rice
Článek Only One Isoform of CTP Synthase Forms the Cytoophidium
Článek Mechanisms Involved in the Functional Divergence of Duplicated GroEL Chaperonins in DK1622
Článek A Genome-Wide RNAi Screen in Identifies the Nicotinic Acetylcholine Receptor Subunit ACR-7 as an Antipsychotic Drug Target
Článek Autophagy Induction Is a Tor- and Tp53-Independent Cell Survival Response in a Zebrafish Model of Disrupted Ribosome Biogenesis
Článek Ancient DNA Reveals Prehistoric Gene-Flow from Siberia in the Complex Human Population History of North East Europe
Článek Inflammation-Mediated Genetic and Epigenetic Alterations Drive Cancer Development in the Neighboring Epithelium upon Stromal Abrogation of TGF-β Signaling
Článek MicroRNA-3148 Modulates Allelic Expression of Toll-Like Receptor 7 Variant Associated with Systemic Lupus Erythematosus
Článek RNAi–Based Functional Profiling of Loci from Blood Lipid Genome-Wide Association Studies Identifies Genes with Cholesterol-Regulatory Function
Článek CELF Family RNA–Binding Protein UNC-75 Regulates Two Sets of Mutually Exclusive Exons of the Gene in Neuron-Specific Manners in
Článek Coordination of Chromatid Separation and Spindle Elongation by Antagonistic Activities of Mitotic and S-Phase CDKs
Článek The Ubiquitin Ligase Subunit Acts in Target Tissue to Restrict Tracheal Terminal Cell Branching and Hypoxic-Induced Gene Expression
Článek Mitotic Evolution of Shows a Stable Core Genome but Recombination in Antigen Families
Článek Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility
Článek A Regulatory Pathway, Ecdysone-Transcription Factor Relish-Cathepsin L, Is Involved in Insect Fat Body Dissociation
Článek PcG-Mediated Higher-Order Chromatin Structures Modulate Replication Programs at the BX-C
Článek MSH3 Polymorphisms and Protein Levels Affect CAG Repeat Instability in Huntington's Disease Mice
Článek JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes
Článek Discovery of a Splicing Regulator Required for Cell Cycle Progression
Článek Rearrangements of 2.5 Kilobases of Noncoding DNA from the Locus Define Predictive Rules of Genomic -Regulatory Logic
Článek Admixture Mapping in Lupus Identifies Multiple Functional Variants within IFIH1 Associated with Apoptosis, Inflammation, and Autoantibody Production
Článek Roles of the Developmental Regulator Homothorax in Limiting Longevity in
Článek miR-199a-5p Is Upregulated during Fibrogenic Response to Tissue Injury and Mediates TGFbeta-Induced Lung Fibroblast Activation by Targeting Caveolin-1
Článek A Kinome-Wide RNAi Screen in Glia Reveals That the RIO Kinases Mediate Cell Proliferation and Survival through TORC2-Akt Signaling in Glioblastoma
Článek Assembly of the Auditory Circuitry by a Genetic Network in the Mouse Brainstem
Článek SOX2 Co-Occupies Distal Enhancer Elements with Distinct POU Factors in ESCs and NPCs to Specify Cell State
Článek Retrotransposon Activates Ectopic Expression: A Short Tail
Článek Confounding by Repetitive Elements and CpG Islands Does Not Explain the Association between Hypomethylation and Genomic Instability
Článek Cell Reprogramming Requires Silencing of a Core Subset of Polycomb Targets
Článek Properties and Modeling of GWAS when Complex Disease Risk Is Due to Non-Complementing, Deleterious Mutations in Genes of Large Effect
Článek Essential Developmental, Genomic Stability, and Tumour Suppressor Functions of the Mouse Orthologue of
Článek Conditional Inactivation of the DNA Damage Response Gene in Mouse Testis Reveals Separable Roles for Components of the RAD9-RAD1-HUS1 Complex in Meiotic Chromosome Maintenance
Článek Genome-Wide Analysis Points to Roles for Extracellular Matrix Remodeling, the Visual Cycle, and Neuronal Development in Myopia
Článek Patterning of Leaf Vein Networks by Convergent Auxin Transport Pathways
Článek An Evolutionary Perspective on Epistasis and the Missing Heritability
Článek A Retrotransposon Insertion in the 5′ Regulatory Domain of Ptf1a Results in Ectopic Gene Expression and Multiple Congenital Defects in Danforth's Short Tail Mouse
Článek The Mub1/Ubr2 Ubiquitin Ligase Complex Regulates the Conserved Dsn1 Kinetochore Protein
Článek Mutations Can Cause Enamel-Renal Syndrome (ERS)
Článek Yemanuclein and HIRA Cooperate for Assembly of H3.3-Containing Nucleosomes in the Male Pronucleus
Článek Hepatocyte Growth Factor, a Determinant of Airspace Homeostasis in the Murine Lung
Článek ISWI and CHD Chromatin Remodelers Bind Promoters but Act in Gene Bodies
Článek COM-1 Promotes Homologous Recombination during Meiosis by Antagonizing Ku-Mediated Non-Homologous End Joining
Článek Control of Multicellular Development by the Physically Interacting Deneddylases DEN1/DenA and COP9 Signalosome
Článek Antagonism Versus Cooperativity with TALE Cofactors at the Base of the Functional Diversification of Hox Protein Function
Článek Dynamic Association of NUP98 with the Human Genome
Článek Ectopic Expression of Induces Spinal Defects, Urogenital Defects, and Anorectal Malformations in Mice
Článek Regulation of Contributes to the Lineage Potential of Neurogenin3+ Endocrine Precursor Cells in the Pancreas
Článek Gene-Based Testing of Interactions in Association Studies of Quantitative Traits
Článek The Amidation Step of Diphthamide Biosynthesis in Yeast Requires , a Gene Identified through Mining the - Interaction Network
Článek Plant-Symbiotic Fungi as Chemical Engineers: Multi-Genome Analysis of the Clavicipitaceae Reveals Dynamics of Alkaloid Loci
Článek Genome-Wide Diversity in the Levant Reveals Recent Structuring by Culture
Článek DNA Methylation Mediated Control of Gene Expression Is Critical for Development of Crown Gall Tumors
Článek Identification of the SlmA Active Site Responsible for Blocking Bacterial Cytokinetic Ring Assembly over the Chromosome
Článek Expression of a Novel P22 ORFan Gene Reveals the Phage Carrier State in Typhimurium
Článek Altered Cohesin Gene Dosage Affects Mammalian Meiotic Chromosome Structure and Behavior
Článek Quantitative Analysis of Histone Modifications: Formaldehyde Is a Source of Pathological N-Formyllysine That Is Refractory to Histone Deacetylases
Článek Duplicate Abalone Egg Coat Proteins Bind Sperm Lysin Similarly, but Evolve Oppositely, Consistent with Molecular Mimicry at Fertilization
Článek Lessons from on the Strengths and Weaknesses of Structured Association Mapping
Článek DNA–Methylome Analysis of Mouse Intestinal Adenoma Identifies a Tumour-Specific Signature That Is Partly Conserved in Human Colon Cancer
Článek Transposon Variants and Their Effects on Gene Expression in
Článek Polygenic Modeling with Bayesian Sparse Linear Mixed Models
Článek The JNK Signaling Pathway Activates Expression of Stress Response Genes by Derepressing the Fos/HDAC Repressor Complex
Článek The Interaction of CtIP and Nbs1 Connects CDK and ATM to Regulate HR–Mediated Double-Strand Break Repair
Článek Regulation of Metamorphosis by Xenobiotic Response Regulators
Článok vyšiel v časopise

PLOS Genetics

2013 Číslo 2
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

nový kurz
Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#