Phenylpropenoic Acid Glucoside from Rooibos Protects Pancreatic Beta Cells against Cell Death Induced by Acute Injury

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Objective:
Previous studies demonstrated that a phenylpropenoic acid glucoside (PPAG) from rooibos (Aspalathus linearis) extract had anti-hyperglycemic activity and significant protective effects on the pancreatic beta cell mass in a chronic diet-induced diabetes model. The present study evaluated the cytoprotective effect of the phytochemical on beta cells exposed to acute cell stress.

Methods:
Synthetically prepared PPAG was administered orally in mice treated with a single dose of streptozotocin to acutely induce beta cell death and hyperglycemia. Its effect was assessed on beta cell mass, proliferation and apoptotic cell death. Its cytoprotective effect was also studied in vitro on INS-1E beta cells and on human pancreatic islet cells.

Results:
Treatment with the phytochemical PPAG protected beta cells during the first days after the insult against apoptotic cell death, as evidenced by TUNEL staining, and prevented loss of expression of anti-apoptotic protein BCL2 in vivo. In vitro, PPAG protected INS-1E beta cells from streptozotocin-induced apoptosis and necrosis in a BCL2-dependent and independent way, respectively, depending on glucose concentration. PPAG also protected human pancreatic islet cells against the cytotoxic action of the fatty acid palmitate.

Conclusions:
These findings show the potential use of PPAG as phytomedicine which protects the beta cell mass exposed to acute diabetogenic stress.

Autoři: Eddy Himpe ^1*; Daniel A. Cunha ²; Imane Song ¹; Marco Bugliani ⁴; Piero Marchetti ⁴; Miriam Cnop ^2,3; Luc Bouwens ¹
Působiště autorů: Cell Differentiation Lab, Diabetes Research Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium ¹; ULB Center for Diabetes Research, Université Libre de Bruxelles (ULB), Brussels, Belgium ²; Division of Endocrinology, Erasmus Hospital, Brussels, Belgium ³; Department of Endocrinology and Metabolism, University of Pisa, Pisa, Italy ⁴
Vyšlo v časopise: PLoS ONE 11(6)
Kategorie: Research article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0157604

© 2016 Himpe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The electronic version of this article is the complete one and can be found online at: http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0157604

Souhrn

Conclusions:
These findings show the potential use of PPAG as phytomedicine which protects the beta cell mass exposed to acute diabetogenic stress.

Zdroje

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