Genetic Regulation by NLA and MicroRNA827 for Maintaining
Nitrate-Dependent Phosphate Homeostasis in
Plants need abundant nitrogen and phosphorus for higher yield. Improving plant
genetics for higher nitrogen and phosphorus use efficiency would save
potentially billions of dollars annually on fertilizers and reduce global
environmental pollution. This will require knowledge of molecular regulators for
maintaining homeostasis of these nutrients in plants. Previously, we reported
that the NITROGEN LIMITATION ADAPTATION (NLA)
gene is involved in adaptive responses to low-nitrogen conditions in
Arabidopsis, where nla mutant plants
display abrupt early senescence. To understand the molecular mechanisms
underlying NLA function, two suppressors of the
nla mutation were isolated that recover the
nla mutant phenotype to wild type. Map-based cloning
identified these suppressors as the phosphate (Pi) transport-related genes
PHF1 and PHT1.1. In addition,
NLA expression is shown to be regulated by the low-Pi
induced microRNA miR827. Pi analysis revealed that the early senescence in
nla mutant plants was due to Pi toxicity. These plants
accumulated over five times the normal Pi content in shoots specifically under
low nitrate and high Pi but not under high nitrate conditions. Also the Pi
overaccumulator pho2 mutant shows Pi toxicity in a
nitrate-dependent manner similar to the nla mutant. Further,
the nitrate and Pi levels are shown to have an antagonistic crosstalk as
displayed by their differential effects on flowering time. The results
demonstrate that NLA and miR827 have pivotal roles in
regulating Pi homeostasis in plants in a nitrate-dependent fashion.
Vyšlo v časopise:
Genetic Regulation by NLA and MicroRNA827 for Maintaining
Nitrate-Dependent Phosphate Homeostasis in. PLoS Genet 7(3): e32767. doi:10.1371/journal.pgen.1002021
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pgen.1002021
Souhrn
Plants need abundant nitrogen and phosphorus for higher yield. Improving plant
genetics for higher nitrogen and phosphorus use efficiency would save
potentially billions of dollars annually on fertilizers and reduce global
environmental pollution. This will require knowledge of molecular regulators for
maintaining homeostasis of these nutrients in plants. Previously, we reported
that the NITROGEN LIMITATION ADAPTATION (NLA)
gene is involved in adaptive responses to low-nitrogen conditions in
Arabidopsis, where nla mutant plants
display abrupt early senescence. To understand the molecular mechanisms
underlying NLA function, two suppressors of the
nla mutation were isolated that recover the
nla mutant phenotype to wild type. Map-based cloning
identified these suppressors as the phosphate (Pi) transport-related genes
PHF1 and PHT1.1. In addition,
NLA expression is shown to be regulated by the low-Pi
induced microRNA miR827. Pi analysis revealed that the early senescence in
nla mutant plants was due to Pi toxicity. These plants
accumulated over five times the normal Pi content in shoots specifically under
low nitrate and high Pi but not under high nitrate conditions. Also the Pi
overaccumulator pho2 mutant shows Pi toxicity in a
nitrate-dependent manner similar to the nla mutant. Further,
the nitrate and Pi levels are shown to have an antagonistic crosstalk as
displayed by their differential effects on flowering time. The results
demonstrate that NLA and miR827 have pivotal roles in
regulating Pi homeostasis in plants in a nitrate-dependent fashion.
Zdroje
1. PoirierYBucherM
2002
Phosphate transport and homeostasis in
Arabidopsis;
SomervilleCRMeyerowitzE
American Society of Plant Biologists
Rockville, MD
doi:10.1199/tab.0024,
http://www.aspb.org/publications/Arabidopsis.
2. MarschnerH
1995
Mineral nutrition of higher plants.
London
Academic Press
3. GoodAGShrawatAKMuenchDG
2004
Can less yield more? Is reducing nutrient input into the
environment compatible with maintaining crop production?
Trends Plant Sci
9
12
597
605
4. BarberSAWalkerJMVaseyEH
1963
Mechanisms for movement of plant nutrients from soil and
fertilizer to plant root.
J Agri Food Chem
11
3
204
207
5. RaghothamaKG
1999
Phosphate acquisition.
Annu Rev Plant Physiol Plant Mol Biol
50
665
693
6. LinWYLinSIChiouTJ
2009
Molecular regulators of phosphate homeostasis in
plants.
J Exp Bot
60
5
1427
1438
7. RubioVLinharesFSolanoRMartinACIglesiasJ
2001
A conserved MYB transcription factor involved in phosphate
starvation signaling both in vascular plants and in unicellular
algae.
Genes Dev
15
16
2122
2133
8. MiuraKRusASharkhuuAYokoiSKarthikeyanAS
2005
The Arabidopsis SUMO E3 ligase SIZ1 controls
phosphate deficiency responses.
Proc Natl Acad Sci USA
102
21
7760
7765
9. GonzalezESolanoRRubioVLeyvaAPaz-AresJ
2005
PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 is a plant-specific
SEC12-related protein that enables the endoplasmic reticulum exit of a
high-affinity phosphate transporter in
Arabidopsis.
Plant Cell
17
12
3500
3512
10. ShinHShinHSDewbreGRHarrisonMJ
2004
Phosphate transport in Arabidopsis: Pht1;1 and
Pht1;4 play a major role in phosphate acquisition from both low- and
high-phosphate environments.
Plant J
39
4
629
642
11. MitsukawaNOkumuraSShiranoYSatoSKatoT
1997
Overexpression of an Arabidopsis thaliana high-affinity phosphate
transporter gene in tobacco cultured cells enhances cell growth under
phosphate-limited conditions.
Proceedings of the National Academy of Sciences of the United States of
America
94
13
7098
7102
12. VersawWKHarrisonMJ
2002
A chloroplast phosphate transporter, PHT2;1, influences
allocation of phosphate within the plant and phosphate-starvation
responses.
Plant Cell
14
8
1751
1766
13. DuanKYiKKDangLHuangHJWuW
2008
Characterization of a sub-family of Arabidopsis
genes with the SPX domain reveals their diverse functions in plant tolerance
to phosphorus starvation.
Plant J
54
6
965
975
14. HamburgerDRezzonicoEPetetotJMCSomervilleCPoirierY
2002
Identification and characterization of the Arabidopsis
PHO1 gene involved in phosphate loading to the
xylem.
Plant Cell
14
4
889
902
15. WangYRibotCRezzonicoEPoirierY
2004
Structure and expression profile of the Arabidopsis PHO1 gene
family indicates a broad role in inorganic phosphate
homeostasis.
Plant Physiology
135
1
400
411
16. PoirierYThomaSSomervilleC
Schiefelbein J
1991
A Mutant of Arabidopsis Deficient in Xylem Loading of
Phosphate.
Plant Physiology
97
3
1087
1093
17. AungKLinSIWuCCHuangYTSuCL
2006
pho2, a phosphate overaccumulator, is caused by a nonsense
mutation in a MicroRNA399 target gene.
Plant Physiol
141
3
1000
1011
18. ChiouTJAungKLinSIWuCCChiangSF
2006
Regulation of phosphate homeostasis by microRNA in
Arabidopsis.
Plant Cell
18
2
412
421
19. FujiiHChiouTJLinSIAungKZhuJK
2005
A miRNA involved in phosphate-starvation response in
Arabidopsis.
Curr Biol
15
22
2038
2043
20. CrawfordNMFordeBG
2002
Molecular and developmental biology of inorganic nitrogen
nutrition.;
SomervilleCRMeyerowitzE
American Society of Plant Biologists
Rockville, MD
doi:
10.1199/tab.0011, http://www.aspb.org/publications/Arabidopsis.
21. PengMHannamCGuHLBiYMRothsteinSJ
2007
A mutation in NLA, which encodes a RING-type
ubiquitin ligase, disrupts the adaptability of Arabidopsis
to nitrogen limitation.
Plant J
50
2
320
337
22. PengMHudsonDSchofieldATsaoRYangR
2008
Adaptation of Arabidopsis to nitrogen limitation
involves induction of anthocyanin synthesis which is controlled by the NLA
gene.
J Exp Bot
59
11
2933
2944
23. Chalker-ScottL
1999
Environmental significance of anthocyanins in plant stress
responses.
Photochemistry and Photobiology
70
1
1
9
24. CarringtonJCAmbrosV
2003
Role of microRNAs in plant and animal
development.
Science
301
5631
336
338
25. BartelDP
2004
MicroRNAs: Genomics, biogenesis, mechanism, and
function.
Cell
116
2
281
297
26. PantBDMusialak-LangeMNucPMayPBuhtzA
2009
Identification of nutrient-responsive
Arabidopsis and rapeseed microRNAs by comprehensive
real-time polymerase chain reaction profiling and small RNA
sequencing.
Plant Physiol
150
3
1541
1555
27. JonesJB
1998
Phosphorus toxicity in tomato plants: When and how does it
occur?
Commun Soil Sci Plant Analysis
29
11-14
1779
1784
28. DelhaizeERandallPJ
1995
Characterization of a phosphate-accumulator mutant of
Arabidopsis thaliana.
Plant Physiol
107
1
207
213
29. TinkerPBNyePH
2000
Solute movement in the rhizosphere:
Oxford
Oxford University Press
30. MouradovACremerFCouplandG
2002
Control of flowering time: Interacting pathways as a basis for
diversity.
Plant Cell
14
S111
S130
31. JiangCGaoXLiaoLHarberdNPFuX
2007
Phosphate starvation root architecture and anthocyanin
accumulation responses are modulated by the gibberellin-DELLA signaling
pathway in Arabidopsis.
Plant Physiol
145
4
1460
1470
32. BlazquezMAGreenRNilssonOSussmanMRWeigelD
1998
Gibberellins promote flowering of Arabidopsis by
activating the LEAFY promoter.
Plant Cell
10
5
791
800
33. De AngeliAMonachelloDEphritikhineGFrachisseJMThomineS
2006
The nitrate/proton antiporter AtCLCa mediates nitrate
accumulation in plant vacuoles.
Nature
442
7105
939
942
34. LinkohrBIWilliamsonLCFitterAHLeyserHMO
2002
Nitrate and phosphate availability and distribution have
different effects on root system architecture of
Arabidopsis.
Plant J
29
6
751
760
35. SmalleJVierstraRD
2004
The ubiquitin 26S proteasome proteolytic pathway.
Annu Rev Plant Biol
55
555
590
36. LukowitzWGillmorCSScheibleWR
2000
Positional cloning in Arabidopsis. Why it feels
good to have a genome initiative working for you.
Plant Physiol
123
3
795
805
37. EarleyKWHaagJRPontesOOpperKJuehneT
2006
Gateway-compatible vectors for plant functional genomics and
proteomics.
Plant J
45
4
616
629
38. CloughSJBentAF
1998
Floral dip: a simplified method for Agrobacterium-mediated
transformation of Arabidopsis thaliana.
Plant J
16
6
735
743
39. AmesBN
1966
Assay of inorganic phosphate, total phosphate and
phosphatases.
Methods Enzymol
8
115
118
40. KantSKantPRavehEBarakS
2006
Evidence that differential gene expression between the halophyte,
Thellungiella halophila, and Arabidopsis
thaliana is responsible for higher levels of the compatible
osmolyte proline and tight control of Na+ uptake in
T. halophila.
Plant Cell Environ
29
7
1220
1234
41. LivakKJSchmittgenTD
2001
Analysis of relative gene expression data using real-time
quantitative PCR and the 2−ΔΔCΤ
method.
Methods
25
4
402
408
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Genetika Reprodukčná medicínaČlánok vyšiel v časopise
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