Invertase in Immobilized Poppy Cells
INVERTÁZA V IMOBILIZOVANÝCH BUNKÁCH MAKU
Suspenzné kultúry maku siateho (Papaver somniferum L.) sa permeabilizovali Tweenom 80 a imo - bilizovali glutaraldehydom. pH optimum invertázy v imobilizovaných bunkách je 4,6 a tepelné optimum je pri 50 °C. Enzýmová reakcia má lineárny priebeh 5 hodín a dosahuje 69 % konverzie. Imobilizované bunky majú vysokú invertázovú aktivitu a výhodné mechanické vlastnosti.
Klíčová slova:
invertáza – suspenzné bunkové kultúry – imobilizácia – permeabilizácia – mak siaty (Papaver somniferum L.)
Authors:
J. Stano 1; P. Kovács 2; K. Neubert 3
Authors‘ workplace:
Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava 3 Biochemický ústav Univerzity Martina Luthera, Halle/Saale
1
Published in:
Čes. slov. Farm., 1999; , 30-33
Category:
Overview
Cell suspension culture of the opium poppy plant (Papaver somniferum L.) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The pH optimum of the immobilized cells’ invertase is 4.6 and the temperature optimum is 50 °C. The enzyme hydrolysis was linear for 5 h, reaching 69 % of conversion. The immobilized cells are characterized by a high invertase activity and showed convenient mechanical properties.
Key words:
invertase – cell suspension culture – immobilization – permeabilization – opium poppy (Papaver somniferum L.)
Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
1999 Issue 1
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